2009 Fiscal Year Final Research Report
In vitro synthesis of cell wall polysaccharides and their characterization
| Project/Area Number |
19208017
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産科学・木質工学
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| Research Institution | Kyoto University |
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Principal Investigator |
SUGIYAMA Junji Kyoto University, 生存圏研究所, 教授 (40183842)
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| Co-Investigator(Kenkyū-buntansha) |
IMAI Tomoya 京都大学, 生存圏研究所, 准教授 (90509142)
BABA Keiichi 京都大学, 生存圏研究所, 助教 (20238223)
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| Co-Investigator(Renkei-kenkyūsha) |
WADA Masahisa 東京大学, 大学院・農学生命科学研究科, 准教授 (40270897)
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| Project Period (FY) |
2007 – 2009
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| Keywords | セルロース / バイオマス / セルロース合成酵素 / 膜タンパク質 / BY-2 / 酢酸菌 / 緑藻 |
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| Research Abstract |
In vitro synthesis of cellulose was conducted with several cellulose-producing organisms. When green algae Micrasterias crux-melientes used, β1→3-D-glucan was the major product as well as plant cultured cells used. We reconfirmed by electron diffraction, X-ray diffraction and FTIR that cellulose II was synthesized in vitro when Gluconacetobacter xylinus (vinegar-producing bacterium) used, as shown previously. Noticeably, it was shown by MALDI-ToF MS for the first time that this cellulose was not oligomer like many of artificially synthesized cellulose, but probably polymer. It is thus concluded that solubilization by detergent does not damage seriously the cellulose synthesizing activity of G. xylinus.
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