2010 Fiscal Year Final Research Report
Spatio-temporal analysis of small GTPases involved in vesicular trafficking by fluorescence imaging
| Project/Area Number |
19209008
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKESHI Nakamura 東京理科大学, 教授 (60362604)
KIYOKAWA Etsuko 金沢医科大学, 医学部, 教授 (80300929)
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| Project Period (FY) |
2007 – 2010
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| Keywords | FRET / G蛋白 / R-Ras / Rab5 / Rac / Ral / アポトーシス / 小胞輸送 |
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| Research Abstract |
Vesicular traffic regulates not only the process essential for cell survival but also intercellular and intracellular signaling. To elucidate the regulatory mechanism of vesicular traffic, we developed biosensors based on the principle of fluorescent resonance energy transfer, and succeeded for the first time to visualize the activity change of low-molecular weight GTPases on the vesicles. Furthermore, we identified molecules that regulate the activity of such low-molecular weight GTPases.
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Research Products
(37 results)
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[Journal Article] SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility2011
Author(s)
Parrini MC, Sadou-Dubourgnoux A, Aoki K, Kunida K, Biondini M, Hatzoglou A, Poullet P, Formstecher E, Yeaman C, Matsuda M, Rosse C, Camonis J
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Journal Title
Mol. Cell
Volume: 42
Pages: 650-661
Peer Reviewed
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[Journal Article] R-Ras regulates exocytosis by Rgl2/Rlf-mediated activation of RalA on endosomes2007
Author(s)
Takaya A, Kamio A, Masuda M, Mochizuki N, Sawa H, Sato M, Nagashima K, Mizutani A, Matsuno A, Kiyokawa E, Matsuda M
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Journal Title
Mol. Biol. Cell
Volume: 18
Pages: 1850-1860
Peer Reviewed
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