2010 Fiscal Year Final Research Report
Development of next generation nano-biomaterials by cell surface engineering
| Project/Area Number |
19300176
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Keio University |
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Principal Investigator |
FUJIMOTO Keiji Keio University, 理工学部, 教授 (70229045)
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| Project Period (FY) |
2007 – 2010
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| Keywords | 細胞 / 組織工学 |
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| Research Abstract |
We tried an approach to construct 2- and 3-dimensional tissues using a ligand-immobilized polymer chain as an artificial extracellular matrix. This technique based on the cell-agglomerate engineering allowed the efficient cellular coating of artificial materials with complicated shapes for improvement of biocompatibility and facilitation of tissue reconstruction. Next, we proposed a novel method for enhancing ligand-receptor binding and modifying cell surface as artificial material. So, we prepared a multi-functional polymer (polymer nano-tool) consisted of ligand, photo-reactive group, biotin and disulfide. We found that cells modified with biotin-carrying nano-tools specifically adhered to an avidin-immobilized plate and the formation of cell agglomerates was mediated by adding avidin.
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Research Products
(9 results)
