Structure and function of metal-containing sensor proteins that use gas molecules as physiological effectors

2009 Fiscal Year Final Research Report

• Project/Area Number: 19370059
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2009); National Institutes of Natural Sciences Okazaki Research Facilities (2007-2008)
• Principal Investigator: AONO Shigetoshi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (60183729)
• Project Period (FY): 2007 – 2009
• Keywords: センサータンパク質 / ヘムタンパク質 / 構造機能相関 / シグナル伝達

Research Abstract

I have studied gas sensor proteins using oxygen or carbon monoxide as their physiological effector molecules to elucidate their structure and function relationships. This study has revealed a novel biological function of gas molecules as signal molecules and the molecular mechanism of gas sensor proteins.

Research Products

• [Journal Article] The role of the Fe-S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii (2010)
  • Author(s): H. Nakajima, N. Takatani, K. Yoshimitsu, M. Ito, S. Aono, Y. Takahashi, Y. Watanabe
  • Journal Title: FEBS J. 277 Pages: 817-832
  • Peer Reviewed
• [Journal Article] Molecular Oxygen regulates the enzymatic activity of a heme-containing diguanylate cyclase (HemDGC) for the synthesis of cyclic di-GMP (2010)
  • Author(s): H. Sawai, S. Yoshioka, T. Uchida, M. Hyodo, Y. Hayakawa, K. Ishimori, S. Aono
  • Journal Title: Biochim. Biophys. Acta-Proteins and Proteomics 1804 Pages: 166-172
  • Peer Reviewed
• [Journal Article] X-ray crystal structure of Michaelis complex of aldoxime dehydratase (2009)
  • Author(s): H. Sawai, H. Sugimoto, Y. Asano, Y. Kato, Y. Shiro, S. Aono
  • Journal Title: J. Biol. Chem. 284 Pages: 32089-32096
  • Peer Reviewed
• [Journal Article] Metal-containing sensor proteins sensing diatomic gas molecules (2008)
  • Author(s): S. Aono
  • Journal Title: Dalton Trans. -24 Pages: 3137-3146
  • Peer Reviewed
• [Journal Article] Hydrogen bonding interaction on the heme-bound ligand in the heme-based O2 sensor protein (2008)
  • Author(s): M. Nishimura, H. Yoshimura, K. Ozawa, S. Yoshioka, M. Kubo, T. Kitagawa, S. Aono
  • Journal Title: J. Porphyrins Phthalocyanines 12 Pages: 142-148
  • Peer Reviewed
• [Journal Article] Protein conformation changes of HemAT-Bs upon ligand binding probed by ultraviolet resonance Raman spectroscopy (2008)
  • Author(s): Samir F. El-Mashtoly, Y. Gu, H. Yoshimura, S. Yoshioka, S. Aono, T. Kitagawa
  • Journal Title: J. Biol. Chem. 283 Pages: 6942-6949
  • Peer Reviewed
• [Journal Article] The formation of hydrogen bond in the proximal heme pocket of HemAT-Bs upon ligand binding (2007)
  • Author(s): H. Yoshimura, S. Yoshioka, Y. Mizutani, S. Aono
  • Journal Title: Biochem. Biophys. Res. Commun. 357(4) Pages: 1053-1057
  • Peer Reviewed
• [Presentation] Molecular oxygen regulates the enzymatic activity of a heme-containing diguanylate cyclase, HemDGC (2009)
  • Author(s): S. Aono
  • Organizer: 14th International Conference on Biological Inorganic Chemistry (ICBIC-14)
  • Place of Presentation: 名古屋国際会議場(愛知)
  • Year and Date: 2009-07-30
• [Presentation] Nitrogenase transcriptional activator, VnfA-Identification of signal molecule by using in vivo activity assay system (2009)
  • Author(s): K. Yoshimitsu
  • Organizer: 14th International Conference on Biological Inorganic Chemistry (ICBIC-14)
  • Place of Presentation: 名古屋国際会議場(愛知)
  • Year and Date: 2009-07-26
• [Presentation] Functional analysis of vanadium nitrogenase regulating protein in Azotobacter vinelandii (2009)
  • Author(s): H. Nakajima
  • Organizer: 14th International Conference on Biological Inorganic Chemistry (ICBIC-14)
  • Place of Presentation: 名古屋国際会議場(愛知)
  • Year and Date: 2009-07-26
• [Presentation] Physiological role of thiolate coordination to the heme in CooA from R. rubrum (2009)
  • Author(s): S. Aono
  • Organizer: 16th International Conference on Cytoshrome P450
  • Place of Presentation: 万国津粱館(沖縄)
  • Year and Date: 2009-06-24
• [Presentation] 時間分解共鳴ラマン分光法によるガスセンサータンパク質HemATの酸素脱離に伴うダイナミクスの観測 (2009)
  • Author(s): 吉田祐
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学(千葉)
  • Year and Date: 2009-03-29
• [Presentation] Fe-Sクラスタを有するニトロゲナーゼ転写調節因子VnfAにおけるクラスタの役割 (2009)
  • Author(s): 中島洋
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学(千葉)
  • Year and Date: 2009-03-29
• [Presentation] ニトロゲナーゼ転写調節因子VnfA- in vivoアッセイによる環境因子の同定 (2009)
  • Author(s): 吉満匡平
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学(千葉)
  • Year and Date: 2009-03-27
• [Presentation] Heme-based gas sensor proteins as a possible material for a bioinorganic device (2008)
  • Author(s): S. Aono
  • Organizer: The IUMRS International Conference in Asia 2008 (IUMRS-ICA 2008)
  • Place of Presentation: 名古屋国際会議場(愛知)
  • Year and Date: 2008-12-10
• [Presentation] A novel globin-coupled oxygen sensor protein responsible for the synthesis of a bacterial second messenger, cyclic di-GMP (2008)
  • Author(s): S. Aono
  • Organizer: 4th Asian Biological Inorganic Chemistry Conference (AsBIC-4)
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-11-13
• [Presentation] Functional analyses of Fe3S4 cluster involved in VnfA, vanadium nitrogenase regulation protein (2008)
  • Author(s): H. Nakajima
  • Organizer: 4th Asian Biological Inorganic Chemistry Conference (AsBIC-4)
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-11-13
• [Presentation] ニトロゲナーゼ転写調節因子VnfAに含まれる鉄イオウの役割 (2008)
  • Author(s): 中島洋
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 東京工業大学(神奈川)
  • Year and Date: 2008-09-19
• [Presentation] HemDGC, a novel globin-coupled O2 sensor, regulates synthesis of a bacterial second messenger (c-di-GMP) (2008)
  • Author(s): H. Sawai
  • Organizer: 4th International Conference on Metals and Genetics (ICMG-13)
  • Place of Presentation: Paris, France
  • Year and Date: 2008-07-22
• [Presentation] The molecular mechanism of functional regulation of the heme-based sensor proteins (2008)
  • Author(s): S. Aono
  • Organizer: 5th International Conference on Porphyrins and Phthalocyanines (ICPP-5)
  • Place of Presentation: Moscow, Russia
  • Year and Date: 2008-07-11
• [Presentation] Sensing of gas molecules and signal transduction in coordination space of sensor proteins (2007)
  • Author(s): S. Aono
  • Organizer: 3rd International Symposium on Chemistry of Coordination Space (ISCCS 2007)
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫)
  • Year and Date: 2007-12-10
• [Presentation] セカンドメッセンジャー(c-di-GMP)合成を制御する新規な酸素センサータンパク質の構造と性質 (2007)
  • Author(s): 吉岡資郎
  • Organizer: 第40回酸化反応討論会
  • Place of Presentation: 奈良女子大学(奈良)
  • Year and Date: 2007-11-17
• [Presentation] Molecular mechanism by which heme-based sensor proteins sense their effector gas molecules (2007)
  • Author(s): S. Aono
  • Organizer: 67th Okazaki Conference「Molecular Science and Chemical Biology of Biomolecular Function」
  • Place of Presentation: 岡崎コンファレンスセンター(愛知)
  • Year and Date: 2007-11-11
• [Presentation] 酸素センサータンパク質HemATによるCheAリン酸化反応の制御 (2007)
  • Author(s): 西村宗十
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 東北大学(宮城)
  • Year and Date: 2007-09-28
• [Presentation] ニトロゲナーゼ転写調節因子VnfAが有するFe-Sクラスタによる転写調節の仕組み (2007)
  • Author(s): 中島洋
  • Organizer: 第57回錯体化学討論会
  • Place of Presentation: 名古屋工業大学(愛知)
  • Year and Date: 2007-09-25
• [Presentation] 酸素センサータンパク質HemATによる特異的酸素認識とシグナル伝達 (2007)
  • Author(s): 青野重利
  • Organizer: 第100回触媒化学討論会A
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2007-09-19
• [Presentation] The mechanism of CO sensing in the heme-based CO sensor CooA revealed by the crystal structure of the exogenous ligand bound form (2007)
  • Author(s): S. Aono
  • Organizer: 13th International Conference on Biological Inorganic Chemistry (ICBIC-13)
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2007-07-17
• [Presentation] Oxygen sensing mechanism of signal transducer protein HemAT (2007)
  • Author(s): M. Nishimura
  • Organizer: 13th International Conference on Biological Inorganic Chemistry (ICBIC-13)
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2007-07-17
• [Presentation] グロビン型センサードメインを有するセカンドメッセンジャー(c-di-GMP)合成酵素の酸素による活性制御 (2007)
  • Author(s): 澤井仁美
  • Organizer: 第34回生体分子科学討論会
  • Place of Presentation: 東北大学(宮城)
  • Year and Date: 2007-06-22
• [Presentation] 気体分子により駆動される生体内シグナル伝達の分子機構 (2007)
  • Author(s): 青野重利
  • Organizer: 第7回日本蛋白質科学会年会
  • Place of Presentation: 仙台国際センター(宮城)
  • Year and Date: 2007-05-26
• [Remarks]
  • URL: http://www.oib.orion.ac.jp/Lab/aono.html