2010 Fiscal Year Final Research Report
Study on photosynthetic water oxidation chemistry in photosystem II
| Project/Area Number |
19370061
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Ibaraki University |
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Principal Investigator |
ONO Takaaki Ibaraki University, 工学部, 教授 (10175268)
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| Co-Investigator(Kenkyū-buntansha) |
MINO Hiroyuki 名古屋大学, 理学研究科, 准教授 (70300902)
MIZUSAWA Naoki 東京大学, 総合文化研究科, 助教 (80342856)
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| Project Period (FY) |
2007 – 2010
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| Keywords | 光生物 |
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| Research Abstract |
Fourier transform infrared(FTIR) spectra for reaction intermediates in photosynthetic water oxidation were measure at low frequency in ^<17>O and ^<18>O raveled water in PSII core samples were prepared from thermophilic cyanobacterium Thermocynechococcus elongatus and the infrared bands responsible for direct interaction between Mn and substrate water were assigned in every reaction intermediate states of the water oxidation. FTIR spectra were also measured in site-directed mutants of Synechocystis sp.PCC6803. Based on the results, roles of D1 C-terminal alanine as a ligand were evaluated.
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