Molecular mechanism that induces cytokinesis

2009 Fiscal Year Self-evaluation Report

• Project/Area Number: 19370080
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Gakushuin University
• Principal Investigator: MABUCHI Issei Gakushuin University, 理学部, 教授 (40012520)
• Project Period (FY): 2007 – 2009
• Keywords: 細胞 / タンパク質 / 生体分子 / シグナル伝達 / 電子顕微鏡 / 細胞質分裂 / 収縮環 / アクチンフィラメント

Research Abstract

細胞分裂は生物の増殖・再生にとって必須の過程であり、分裂面の決定は多細胞生物の発生・分化にとって重要である。動物細胞や酵母は分裂期に分裂面の細胞膜直下に形成される「収縮環」の収縮により分裂する。この構造は主としてアクチンフィラメントとII型ミオシン(以下ミオシン)から成り、これらの相互作用によって収縮する。収縮環の形成位置(細胞の分裂位置)の決定機構、収縮環の形成機構の問題はこの分野の中心的課題である。そこで分裂シグナル伝達の機構とそれを受けて収縮環がどのように形成され、収縮するかを分子レベルで解明したい。

Research Products

• [Journal Article] Elongation factors are involved in cytokinesis of sea urchin eggs. (2010)
  • Author(s): Fujimoto, H., Mabuchi, I.
  • Journal Title: Genes Cells 15 Pages: 23-135
  • Peer Reviewed
• [Journal Article] Calyculin-A induces cleavage in a random plane in unfertilized sea urchin eggs. (2009)
  • Author(s): Goda, M., Inoue, S., Mabuchi, I.
  • Journal Title: Biol. Bull. 216 Pages: 40-44
  • Peer Reviewed
• [Journal Article] In vivo phosphorylation of regulatory light chain of myosin II in sea urchin eggs and its role in controlling myosin localization and function during cytokinesis. (2008)
  • Author(s): Uehara, R., Hosoya, H., Mabuchi, I.
  • Journal Title: Cell Motil. Cytoskelet. 65 Pages: 100-115
  • Peer Reviewed
• [Journal Article] Properties of actin from the fission yeast Schizosaccharomyces pombe and interaction with fission yeast profilin. (2007)
  • Author(s): Takaine, M., Mabuchi, I.
  • Journal Title: J.Biol. Chem. 282 Pages: 21683-21694
  • Peer Reviewed
• [Journal Article] IQGAP2 is required for the cadherin-mediated cell-to-cell adhesion in Xenopus laevis embryos. (2007)
  • Author(s): Yamashiro S., Abe, H., Mabuchi, I.
  • Journal Title: Dev. Biol 308 Pages: 485-493
  • Peer Reviewed
• [Journal Article] Three-dimensional arrangement of F-actin in the contractile ring of fission yeast. (2007)
  • Author(s): Kamasaki, T., Osumi, M., Mabuchi, I
  • Journal Title: J.Cell Biol 178 Pages: 765-771
  • Peer Reviewed
• [Journal Article] Characterization of native myosin VI isolated from sea urchin eggs. (2007)
  • Author(s): Sakata, S., Watanabe, Y., Usukura, J., Mabuchi, I
  • Journal Title: J.Biochem. 142 Pages: 481-490
  • Peer Reviewed
• [Presentation] Mitochondria are anchored by actin filaments to the cortex and are motile in sea urchin eggs. (2009)
  • Author(s): Mabuchi, I., Takagi, T., Inoue, S., Goda, M.
  • Organizer: The 50th ASCB Annual Meeting
  • Place of Presentation: San Diego, USA.
  • Year and Date: 20091200
• [Presentation] Structure and formation of the contractile ring in fission yeast. (2009)
  • Author(s): Mabuchi, I., Kamasaki, T., Oiwa, Y., Takagi, T., Inoue, S., Shirato, Y., Kashiwazaki, J.
  • Organizer: The 5th International Fission Yeast Meeting
  • Place of Presentation: Tokyo
  • Year and Date: 20091026-20091031
• [Presentation] Analysis for Klp8, a unique kinesin-like protein in fission yeast. (2009)
  • Author(s): Kashiwazaki, J., Yoneda, Y., Arai, R., Yoshida, M., Mabuchi, I.
  • Organizer: The 5th International Fission Yeast Meeting
  • Place of Presentation: Tokyo
  • Year and Date: 20091026-20091031
• [Presentation] Localization analysis of Klp8, a unique kinesin-like protein of fission yeast. (2009)
  • Author(s): 米田裕美、武藤悌、荒井律子、吉田稔、馬渕一誠
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090602-20090604
• [Presentation] Structure and formation of the contractile ring in fission yeast and animal cells. Symposium : "Structure and evolution of cytokinetic machinery in eukaryotic cells" (2009)
  • Author(s): Kamasaki, T., Oiwa, Y., Takagi, T., Mabuchi, I.
  • Organizer: JSCB 61st Annual Meeting
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-02
• [Presentation] Structural analysis of the contractile ring and other actin cytoskeletons in fission yeast cells. (2008)
  • Author(s): Mabuchi, I.
  • Organizer: Gordon Research Conference on Plant and Fungal Cytoskeletons
  • Place of Presentation: Lucca, Italy
  • Year and Date: 20080803-20080800
• [Presentation] Roles of Mid1/Dmf1 and Plo1 in formation of the contractile ring in fission yeast. (2008)
  • Author(s): 白戸悠香子、武藤悌、馬渕一誠
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20080629-20080701
• [Presentation] Structure and assembly of actin cytoskeletons in fission yeast cell. (2008)
  • Author(s): Mabuchi, I.
  • Organizer: The 2nd International Symposium on Bio-nanosystems
  • Place of Presentation: 東京
  • Year and Date: 2008-10-31
• [Presentation] Properties of acin from the fission yeast Schizosaccharomyces pombe and interaction with fission yeast profilin. (2007)
  • Author(s): Takaine, M., Mabuchi, I.
  • Organizer: The 47th ASCB annual meeting
  • Place of Presentation: Washington, D. C.
  • Year and Date: 20071200
• [Book] 「細胞骨格」.分子生物学イラストレイテッド第3版 (2009)
  • Author(s): 孤嶋慎一郎、馬渕一誠
  • Total Pages: 175-180
  • Publisher: 羊土社
• [Book] 分子細胞生物学辞典 第2版 (2008)
  • Author(s): 馬渕一誠
  • Publisher: 東京化学同人
• [Book] 生物物理学ハンドブック (2007)
  • Author(s): 馬渕一誠
  • Total Pages: 680
  • Publisher: 朝倉書店
• [Book] 「細胞質分裂 : 細胞分裂における収縮環の形成」。シリーズ21世紀の動物科学5「発生」 (2007)
  • Author(s): 馬渕一誠
  • Total Pages: 9-38
  • Publisher: 培風館
• [Book] 「分裂酵母の細胞質分裂」。酵母のすべて (2007)
  • Author(s): 馬渕一誠
  • Total Pages: 256-267
  • Publisher: シュプリンガージャパン