Search of the gene conferring tannin accumulation in persimmon fruit and its utilization for the analysis of genome composition and the seedling selection

2009 Fiscal Year Final Research Report

• Project/Area Number: 19380019
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Kyoto University
• Principal Investigator: YONEMORI Keizo Kyoto University, 農学研究科, 教授 (10111949)
• Co-Investigator(Kenkyū-buntansha): YAMADA Masahiko 果樹研究所, ブドウ・カキ研究チーム, 上席研究員 (00355439) ; SATO Akihiko 果樹研究所, ブドウ・カキ研究チーム, 上席研究員 (30355440) ; TAO Ryutaro 京都大学, 農学研究科, 准教授 (10211997) ; YAMANE Hisayo 京都大学, 農学研究科, 助教 (80335306)
• Co-Investigator(Renkei-kenkyūsha): KANNZAKI Shinya 近畿大学, 農学部, 講師 (20330243)
• Project Period (FY): 2007 – 2009
• Keywords: マメガキ / 倍数体 / 甘渋性決定遺伝子 / 転写因子 / 定量PCR / DNAマーカー

Research Abstract

The gene conferring the trait of astringency-loss in Japanese-type PCNA is shown to be located at 100kb away of fosmid contig constructed from Diospyros lotus, a close relative of D.kaki. This single gene controls the astringency trait in persimmon fruit and 6 alleles are responsible for the appearance of this trait. The DkMyb4, a regulatory gene, is also found to control tannin biosynthesis in the fruit and is indicated to determine whether an individual is PCNA or non-PCNA due to its strength of expression. Using the DNA sequences linked to this gene, we could construct a primer pair for PCR analysis for selecting PCNA offspring of Japanese-type. In addition, we also found an AFLP marker to distinguish Chinese-type PCNA among breeding population.

Research Products

• [Journal Article] SCAR markers for practical application of marker-assisted selection in persimmon (Diospyros kaki Thunb. ) breeding (2010)
  • Author(s): S. Kanzaki, T. Akagi, T. Masuko, M. Kimura, M. Yamada, A. Sato, N. Mitani, N. Utsunomiya, K. Yonemori
  • Journal Title: J. Japan. Soc. Hort. Sci. 79 Pages: 150-155
• [Journal Article] Quantitative genotyping for the astringency locus in hexaploid persimmon cultivars using quantitative real-time PCR (2010)
  • Author(s): T. Akagi, Y. Takeda, K. Yonemori, A. Ikegami, A. Kono, M. Yamada, S. Kanzaki
  • Journal Title: J. Amer. Soc. Hort. Sci. 135 Pages: 59-66
  • Peer Reviewed
• [Journal Article] DkMyb4 is a Myb transcription factor involved in proanthocyanidin biosynthesis in persimmon fruit (2009)
  • Author(s): T. Akagi, A. Ikegami, T. Tsujimoto, S. Kobayashi, A. Sato, A. Kono, K. Yonemori
  • Journal Title: Plant Physiology 151 Pages: 2028-2045
  • Peer Reviewed
• [Journal Article] Construction of a reliable PCR marker for selecting pollination constant and non-astringent (PCNA) type offspring among breeding population of persimmon (Diospyros kaki Thunb. ) (2009)
  • Author(s): K. Yonemori, T. Akagi, S. Kanzaki
  • Journal Title: Acta Horticulturae 839 Pages: 625-629
  • Peer Reviewed
• [Journal Article] Quantitative real-time PCR to determine allele number for the astringency locus by analysis of a linked marker in Diospyros kaki Thunb. (2009)
  • Author(s): T. Akagi, S. Kanzaki, M. Gao, R. Tao, D.E. Parfitt, K. Yonemori
  • Journal Title: Tree Genetics & Genomes 5 Pages: 483-492
  • Peer Reviewed
• [Journal Article] Conversion of RFLP Markers for the Selection of Pollination-Constant and Non-Astringent Type Persimmons (Diospyros kaki Thunb. ) into PCR-Based Markers (2009)
  • Author(s): S. Kanzaki, M. Yamada, A. Sato, N. Mitani, N. Utsunomiya, K. Yonemori
  • Journal Title: J. Japan. Soc. Hort. Sci. 78 Pages: 68-73
  • Peer Reviewed
• [Journal Article] Expression balances of structural genes in shikimate and flovonoid biosynthesis cause a difference in proanthocyanidin accumulation in persimmon (Diospyros kaki Thunb. ) fruit (2009)
  • Author(s): T. Akagi, A. Ikegami, Y. Suzuki, J. Yoshida, M. Yamada, A. Sato,, K. Yonemori
  • Journal Title: Planta 230 Pages: 899-915
  • Peer Reviewed
• [Journal Article] RFLP markers for the selection of pollination-constant and non-astringent (PCNA)-type persimmon and examination of the inheritance mode of the markers (2008)
  • Author(s): S. Kanzaki, A. Sato, M. Yamada, N. Utsunomiya, A. Kitajima, A. Ikegami,, K. Yonemori
  • Journal Title: J. Japan. Soc. Hort. Sci. 77 Pages: 28-32
  • Peer Reviewed
• [Presentation] Identification of the genetic region including astringency (AST) locus in hexaploid persimmon (Diospyros kaki Thunb. ) by positional cloning in a diploid relative, D. lotus (2010)
  • Author(s): T. Tsujimoto, T. Akagi, A. Kono, N. Mitani, K. Yonemori
  • Organizer: Plant and Animal Genome XVIII
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-01-11
• [Presentation] カキの甘渋性を決定するDkMyb4の季節的発現制御因子についての解析 (2009)
  • Author(s): 赤木剛士・池上礼子・小林省三・佐藤明彦・米森敬三
  • Organizer: 園芸学会平成21年度秋季大会
  • Place of Presentation: 秋田大学手形キャンパス
  • Year and Date: 2009-09-26
• [Presentation] カキの甘渋性決定に関与する転写因子DkMyb4の同定 (2009)
  • Author(s): 赤木剛士・池上礼子・佐藤明彦・小林省三・米森敬三
  • Organizer: 園芸学会平成21年度春季大会
  • Place of Presentation: 明治大学駿河台キャンパス
  • Year and Date: 2009-03-19
• [Presentation] Analysis of differentially expressed genes in astringent fruit using suppression subtractive hybridization (2008)
  • Author(s): A. Ikegami, T. Akagi, M. Yamada, A. Kitajima, K. Yonemori
  • Organizer: 4th International Symposium on Persimmon
  • Place of Presentation: Faenza, Italy
  • Year and Date: 2008-11-10
• [Presentation] Molecular characterization of a marker locus linked to the AST gene in persimmon (2008)
  • Author(s): S. Kanzaki, T. Akagi, M. Yamada, N. Mitani, , K. Yonemori
  • Organizer: Plant & Animal Genome XVI
  • Place of Presentation: San Diego California, USA
  • Year and Date: 2008-01-14
• [Presentation] カキ果実で発現するフラボノイド合成系に関連した転写因子群の単離とタンニン蓄積への関与 (2007)
  • Author(s): 赤木剛士・吉田純一・山田昌彦・米森敬三
  • Organizer: 園芸学会平成19年度秋季大会
  • Place of Presentation: 香川大学幸町キャンパス
  • Year and Date: 20070000
• [Presentation] Molecular marker linked to natural astringency-loss in Chinese-type PCNA persimmon (Diospyros kaki Thunb. ) fruit
  • Author(s): K. Yonemori, S. Eguchi, A. Ikegami, Y. Sakaguchi, T. Akagi, A. Kitajima, M. Yamada, , Dan E. Parfitt
  • Organizer: 104th Annual International Conference of the American Society for Horticultural Science
  • Place of Presentation: Scottsdale, Arizona, USA