2009 Fiscal Year Final Research Report
Biosyntheses and molecular functions of novel biofactors, D-amino acids : from microorganisms to mammals
| Project/Area Number |
19380059
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
YOSHIMURA Tohru Nagoya University, 大学院・生命農学研究科, 教授 (70182821)
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| Co-Investigator(Kenkyū-buntansha) |
HEMMI Hisashi 名古屋大学, 大学院・生命農学研究科, 准教授 (60302189)
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| Project Period (FY) |
2007 – 2009
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| Keywords | D-アミノ酸 / D-セリンデヒドラターゼ / D-セリン定量 / ピリドキサルリン酸 / スクレイピー / 細胞性粘菌 / Saccharomyces cerevisiae / Escherichia coli |
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| Research Abstract |
In this study, we found a novel D-serine dehydratase in Saccharomyces cerevisiae, and studied its properties. With this enzyme exhibiting high substrate specificity for D-serine, we constructed a novel D-serine assay system. We examined the relationship between the concentration of D-amino acids in the various organs of a scrapy-infected hamster and the progress in the disease. We also studied the D-serine metabolism in a slime cellular mold, Dictyostellium discoidium, to understand the role of D-serine in development, and found that the cell contains serine racemase catalyzing D-serine synthesis, and D-serine dehydratase and D-amino acid oxidase catalyzing D-serine degradation. In this study, we also examine the function of E.coli YggS, a homolog of the unknown mammalian protein, PROSC, which exhibits the similarity in the primary structure with the bacterial alanine racemase.
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