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2010 Fiscal Year Final Research Report

Molecular breedling for cold stress tolerant forage grasses

Research Project

  • PDF
Project/Area Number 19380148
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionHokkaido University

Principal Investigator

YAMADA Toshihiko  Hokkaido University, 北方生物圏フィールド科学センター, 教授 (70343952)

Co-Investigator(Renkei-kenkyūsha) KANAZAWA Akira  北海道大学, 大学院・農学研究院, 准教授 (30281794)
TAMURA Kenichi  独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター, 研究員 (10414749)
Project Period (FY) 2007 – 2010
Keywords草地利用 / 飼料作物育種
Research Abstract

Rapid and efficient in vitro culture system and novel methods for particle bombardment and Agrobacterium-mediated transformation using mature embryos as explants have been established in zoysiagrass (Zoysia japonica). Fructsyltransferase gene from timothy (PpFT1) involving a synthesis of highly polymerized fructan contributed to increase freezing tolerance by accumulation of fructan and glucose in cold acclimated plants. CBF gene (LpCBFIVa) from perennial ryegrass functioned with an increase of freezing tolerance with delaying flower time.

  • Research Products

    (17 results)

All 2011 2010 2009 2008 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (8 results) Book (3 results)

  • [Journal Article] Molecular mechanisms underlying frost tolerance in perennial grasses adapted to cold climates.2011

    • Author(s)
      S.R.Sandve、A.Kosmala、H.Rudi、S.Fjellheim、M.Rapacz、T.Yamada、O.A.Rognli
    • Journal Title

      Plant Science 180巻

      Pages: 69-77

    • Peer Reviewed
  • [Journal Article] Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonica Steud.)2010

    • Author(s)
      X.Wang、Y.Hoshino、T.Yamada
    • Journal Title

      Grassland Science 56巻

      Pages: 198-204

    • Peer Reviewed
  • [Journal Article] Rapid in vitro culture and Agrobacterium-mediated transformation system in zoysiagrass (Zoysia japonica Steud.)2009

    • Author(s)
      X.Wang、F.J.Kong、Y.Hoshino、T.Yamada、T.Yamada
    • Journal Title

      Proceedings of Joint 14th Austrasian Plant Breeding Conference and 11th SABRAO Congress CD版

    • Peer Reviewed
  • [Journal Article] Transformation of androgenic-derived Festulolium plants (Lolium perenne L. x Festuca pratensis Huds.) by Agrobacterium tumefaciens2009

    • Author(s)
      Y.-D.Guo、H.Hisano、Y.Shimamoto、T.Yamada
    • Journal Title

      Plant Cell Tissue Organ Culture 96巻

      Pages: 219-227

    • Peer Reviewed
  • [Journal Article] Molecular breeding to improve tolerance to abiotic stress2009

    • Author(s)
      T.Yamada
    • Journal Title

      Proceedings on the 1st International Congress on Global Climate Changes and Agriculture、Namik Kemal University, Tekirdag、Turkey

      Pages: 135-149

  • [Journal Article] Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass2008

    • Author(s)
      H.Hisano、A.Kanazawa、M.Yoshida、M.O.Humphreys、M.Iizuka、K.Kitamura、T.Yamada
    • Journal Title

      New Phytologist 178巻

      Pages: 766-780

    • Peer Reviewed
  • [Presentation] Genetic improvement through a transgenic approach in Miscanthus ssp., a new bioenergy crop2010

    • Author(s)
      T.Yamada、X.Wang、A.Kanazawa、T.Yamada、Y.Hoshino、N.Ukaji
    • Organizer
      ASA-CSSA-SSSA 2010 International Annual Meeting
    • Place of Presentation
      アメリカ合衆国、ロングビーチ市
    • Year and Date
      20101031-20101104
  • [Presentation] Molecular breeding to improve tolerance to abiotic stress in forage grasses2010

    • Author(s)
      山田敏彦、田村健一
    • Organizer
      6th International Symposium on the Molecular Breeding of Forage and Turf
    • Place of Presentation
      アルゼンチン、ブエノスアイレス市(招待講演)
    • Year and Date
      20100315-20100318
  • [Presentation] Rapid in vitro callus induction and regeneration of zoysiagrass (Zoysia japonica Steud.) and miscanthus (Miscanthus sinensis Anderss.)2009

    • Author(s)
      X.Wang、Y.Hoshino、T.Yamada
    • Organizer
      第65回日本草地学会
    • Place of Presentation
      藤沢市(日本大学)
    • Year and Date
      20090329-20090330
  • [Presentation] The expression regulation of multiple fructosyltransferase genes controls the fructan accumulation in correspondence with low temperature in perennial ryegrass (Lolium perenne L.)2008

    • Author(s)
      H.Hisano、A.Kanazawa、M.Yoshida、M.Humphreys、M.Iizuka、K.Kitamura、T.Yamada
    • Organizer
      The Sixth International Fructan Symposium
    • Place of Presentation
      札幌市(ホテルモントレ エーデルホフ札幌)
    • Year and Date
      20080728-20080729
  • [Presentation] Low-temperature tolerance related CBF and fructosyltransferase genes in forage grasses2008

    • Author(s)
      H.Hisano、T.Yamada、M.Yoshida、A.Kanazawa、T.Yamada
    • Organizer
      XXI International Grassland Congress
    • Place of Presentation
      中国、フフホト市(招待講演)
    • Year and Date
      20080629-20080705
  • [Presentation] Establishing abundant and efficient explants system for Agrobacterium-mediated transformation in zoysiagrass2008

    • Author(s)
      X.Wang、Y.Hoshino、T.Yamada
    • Organizer
      XXI International Grassland Congress
    • Place of Presentation
      中国、フフホト市
    • Year and Date
      20080629-20080705
  • [Presentation] Functional genomics for winter hardiness in forage grasses2008

    • Author(s)
      H.Hisano、K.Tamura、M.Yoshida、A.Kanazawa、T.Yamada
    • Organizer
      Plant & Animal Genome XVI
    • Place of Presentation
      アメリカ合衆国、サンディエゴ市(招待講演)
    • Year and Date
      2008-01-12
  • [Presentation] チモシーとコムギ由来フルクタン合成酵素遺伝子を導入したイネ科モデル植物Brachypodium distachyon形質転換体の比較解析

    • Author(s)
      田村健一、眞田康治、田瀬和浩、吉田みどり、山田敏彦
    • Organizer
      2011年度日本草地学会
  • [Book] Molecular Techniques in Crop Improvement, 2nd Edition(S.M.Jain and D.S.Brar (eds.))(Transgensis and genomics in forage crops)2010

    • Author(s)
      T.Yamada、K.Tamura、X.Wang、Y.Aoyagi
    • Total Pages
      719-744
    • Publisher
      Springer、the Netherlands
  • [Book] Molecular Breeding of Forage and Turf2009

    • Author(s)
      T.Yamada、G.S.Spangenberg
    • Total Pages
      352
    • Publisher
      Springer、New York
  • [Book] Compendium of Transgenic Crop Plants : Transgenic Cereals and Forage Grasses(C.Kole and T.C.Hall (eds.))(Cool Season Forage Grasses)2008

    • Author(s)
      Z.-Y.Wang、T.Yamada
    • Total Pages
      199-210
    • Publisher
      Blackwell Publishing、Oxford、UK

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Published: 2012-01-26   Modified: 2016-04-21  

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