Elucidation of the molecular mechanism underlying the enhancement of somatic-cell reprogramming by a histone deacetylase inhibitor, and its applications

2009 Fiscal Year Final Research Report

• Project/Area Number: 19380161
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Kinki University
• Principal Investigator: KISHIGAMI Satoshi Kinki University, 生物理工学部, 准教授 (10291064)
• Research Collaborator: TERUHIKO Wakayama 理化学研究所・神戸研究所, チームリーダー (40360672) ; KAZUHIRO Saeki 近畿大学, 生物理工学部, 教授 (10298937)
• Project Period (FY): 2007 – 2009
• Keywords: 発生工学 / 核の初期化

Research Abstract

Somatic-cell nuclear transfer (SCNT) has been extremely inefficient with rates less than 5 percentage points since the first clone, "Dolly" the sheep. The representative researcher for this grant found that treatment of cloned embryos with trichostatin A (TSA), a histone deacetylase inhibitor (HDACi), dramatically increase the production rate of cloned mice more than six-times. Supported by this grant, the importance of HDAC in embryonic development and a mechanism underlying improvement of SCNT by HDACi treatment were revealed and, further cloning of "unclonable" mouse strains were achieved for the first time.

Research Products

• [Journal Article] How to improve the success rate of mouse cloning technology (2010)
  • Author(s): Thuan NV, Kishigami S, Wakayama T
  • Journal Title: J Reprod Dev Vol.56,No.2 Pages: 20-30
  • Peer Reviewed
• [Journal Article] The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice (2009)
  • Author(s): Van Thuan N, Bui HT, Kim JH, Hikichi T, Wakayama S, Kishigami S, Mizutani E, Wakayama T
  • Journal Title: Reproduction Vol.138,No.2 Pages: 309-317
  • Peer Reviewed
• [Journal Article] The cytoplasm of mouse germinal vesicle stage oocytes can enhance somatic cell nuclear reprogramming (2008)
  • Author(s): Bui HT, Wakayama S, Kishigami S, Kim JH, Van Thuan N, Wakayama T
  • Journal Title: Development Vol.135,No.23 Pages: 3935-3945
  • Peer Reviewed
• [Journal Article] Somatic cell nuclear transfer: infinite reproduction of a unique diploid genome (2008)
  • Author(s): Kishigami S, Wakayama S, Hosoi Y, Iritani A, Wakayama T
  • Journal Title: Exp Cell Res Vol.314,No.9 Pages: 1945-1950
  • Peer Reviewed
• [Journal Article] Efficient strontium-induced activation of mouse oocytes in standard culture media by chelating calcium (2007)
  • Author(s): Kishigami S, Wakayama T
  • Journal Title: J Reprod Dev Vol.53,No.6 Pages: 1207-1215
  • Peer Reviewed
• [Journal Article] Regulation of chromatin and chromosome morphology by histone H3 modifications in pig oocytes (2007)
  • Author(s): Bui HT, Van Thuan N, Kishigami S, Wakayama S, Hikichi T, Ohta H, Mizutani E, Yamaoka E, Wakayama T, Miyano T
  • Journal Title: Reproduction Vol.133,No.2 Pages: 371-382
  • Peer Reviewed
• [Journal Article] Successful mouse cloning of an outbred strain by trichostatin A treatment after somatic nuclear transfer. (2007)
  • Author(s): Kishigami S, et al.
  • Journal Title: J Reprod Dev Vol.53,No.1 Pages: 165-170
  • Peer Reviewed
• [Journal Article] Differentiation potential of parthenogenetic embryonic stem cells is improved by nuclear transfer (2007)
  • Author(s): Hikichi T, Wakayama S, Mizutani E, Takashima Y, Kishigami S, Van Thuan N, Ohta H, Thuy Bui H, Nishikawa S, Wakayama T
  • Journal Title: Stem Cells Vol.25,No.1 Pages: 46-53
  • Peer Reviewed
• [Journal Article] Establishment of mouse embryonic stem cell lines from somatic cell nuclei by nuclear transfer into aged, fertilization-failure mouse oocytes (2007)
  • Author(s): Wakayama S, Suetsugu R, Thuan NV, Ohta H, Kishigami S, Wakayama T
  • Journal Title: Curr Biol Vol.17,No.4 Pages: R120-121
  • Peer Reviewed
• [Presentation] A unique property of specified mouse blastomere in Cdx2 expression (2009)
  • Author(s): Kishigami S., et al.
  • Organizer: 6th Asian Reproductive Biotechnology Conference
  • Place of Presentation: Siem Reap, Cambodia
  • Year and Date: 2009-11-24
• [Presentation] The impact of EGTA as chelating calcium on oocyte activation in mice (2008)
  • Author(s): Tsujimoto Y., et al.
  • Organizer: 5th Asian Reproductive Biotechnology Conference
  • Place of Presentation: Yunnan, China
  • Year and Date: 20081126-20081130
• [Presentation] ウシ体細胞核移植胚におけるトリコスタチンA処理の至適時間の検討 (2008)
  • Author(s): 高橋千明, ら
  • Organizer: 平成20年度(第58回大会)関西畜産学会
  • Place of Presentation: 神戸市
  • Year and Date: 20080902-20080903
• [Presentation] Effects of trichostatin A on gene expression and in-vitro development of bovine embryos cloned from transfected fibroblasts carrying a luciferase gene. (2008)
  • Author(s): Iwamoto D, et al.
  • Organizer: 16th International Congress on Animal Reproduction
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 20080713-20080717
• [Presentation] Effects of trichostatin A on DNA methylation in cloned bovine embryos. (2008)
  • Author(s): Iwamoto D, et al.
  • Organizer: 34th Annual Conference of International Embryo Transfer Society
  • Place of Presentation: Denver, USA
  • Year and Date: 20080105-20080109
• [Presentation] オーダーメイド胚培養:移植される核の種類に適した体外培養を考える (2008)
  • Author(s): 岸上哲士
  • Organizer: 第11回日本IVF学会
  • Place of Presentation: 大阪
  • Year and Date: 2008-10-14
• [Presentation] Somatic cell nuclear transfer with HDACi (2008)
  • Author(s): Kishigami S
  • Organizer: 4th JSAR-KSAR Joint Symposium
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2008-06-20
• [Presentation] 発生工学におけるドラッグディスカバリー:クローン技術改善への試み (2007)
  • Author(s): 岸上哲士
  • Organizer: 発生工・疾患モデル研究会
  • Place of Presentation: 東京
  • Year and Date: 20071000
• [Presentation] Histone deacetylases: key regulators to determine the efficiency of reprogramming after somatic-cell nuclear transfer (2007)
  • Author(s): Kishigami S
  • Organizer: 5th Anniversary Congress of International Drug Discovery Science & Technology (IDDST)
  • Place of Presentation: Shanghai, China
  • Year and Date: 20070500
• [Presentation] Abnormal cell adhesion in cloned mouse embryos (2007)
  • Author(s): Suetsugu, S., et al.
  • Organizer: 第40回日本発生生物学会大会(細胞生物学会合同)
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 20070500
• [Presentation] Effects of trichostatin A on development of bovine somatic cell nuclear transfer embryos. (2007)
  • Author(s): Iwamoto D, et al.
  • Organizer: 33th Annual Conference of International Embryo Transfer Society
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20070106-20070110
• [Presentation] Success of mouse cloning from an outbred strain by trichostatin a treatment after somatic nuclear transfer (2007)
  • Author(s): Kishigami S., et al.
  • Organizer: 3rd Annual Conference of the International Embryo Transfer Society (IETS)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20070106-20070110
• [Presentation] Efficient strontium-induced activation of mouse oocytes in standard culture media by chelating calcium (2007)
  • Author(s): Kishigami S., Wakayama T
  • Organizer: 4th Asian Reproductive Biotechnology Conference
  • Place of Presentation: Singapore
  • Year and Date: 2007-11-24
• [Presentation] Oocyte activation by strontium in the presence of calcium supports full- term development of somatic cell cloned embryos (2007)
  • Author(s): Kishigami S., Wakayama T
  • Organizer: 40th Annual meeting of the Society of Reproduction (SSR)
  • Place of Presentation: San Antonio, TX, USA
  • Year and Date: 2007-07-21
• [Book] Nuclear Transfer with HDACi as a New Cloning Method((Lucas T. Dahnof編)) (2010)
  • Author(s): Satoshi Kishigami, Daisaku Iwamoto, Kazuhiro Saeki, Kazuya Matsumoto, Akira Iritani, Yoshihiko Hosoi著
  • Total Pages: 329-334
  • Publisher: Nova Science Pub Inc
• [Book] 体細胞核移植におけるリプログラミング促進技術の開発 (2009)
  • Author(s): 岸上哲士, 矢持隆之, 松原圭吾, 細井美彦著
  • Total Pages: 43-46
  • Publisher: メディカルレビュー社
• [Book] 核移植とiPS細胞技術の比較:リプログラミングの効率化を目指して (2009)
  • Author(s): 岸上哲士, 辻本賀子, 矢持隆之, 細井美彦著
  • Total Pages: 223-227
  • Publisher: 秀潤社
• [Book] Gene knockout Protocols (Methods in Molecular Biologyシリーズ, Vol. 530) (Wolfgang Wurst編) (2009)
  • Author(s): Sayaka Wakayama, Satoshi Kishigami, Teruhiko Wakayama著
  • Total Pages: 516
  • Publisher: Humana Press
• [Book] 体細胞核移植におけるリプログラミング促進技術の開発 (2008)
  • Author(s): 岸上哲士, 細井美彦著
  • Total Pages: 270-273
  • Publisher: メディカルレビュー社
• [Book] Microinjection: Methods and Applications (Methods in Molecular Biologyシリーズ(David Carrol編) (2008)
  • Author(s): Satoshi Kishigami, Teruhiko Wakayama著
  • Total Pages: 224
  • Publisher: Humana Press
• [Book] Genetic and Epigenetic Control of Mammalian Germ Cell Development and Function (Masami Nozaki編) (2008)
  • Author(s): Satoshi Kishigami, Hiroshi Ohta, Teruhiko Wakayama
  • Total Pages: 133-152
• [Book] Progress in DNA Methylation Research (Hans P. Neumann編) (2008)
  • Author(s): Satoshi Kishigami著
  • Total Pages: 256
  • Publisher: Nova Science Publishers Inc
• [Book] 体細胞核移植クローンとクローンES細胞の樹立 (2007)
  • Author(s): 若山静香, 岸上哲士, 若山照彦著
  • Total Pages: 2197-2202
  • Publisher: 共立出版
• [Patent(Industrial Property Rights)] 核移植卵子の作製方法 (2006)
  • Inventor(s): 岸上哲士
  • Industrial Property Rights Holder: 独立行政法人理化学研究所、学校法人近畿大学,独立行政法人科学技術振興機構
  • Industrial Property Number: PCT出願・PCT/JP2006/319311
  • Filing Date: 2006-09-28