2009 Fiscal Year Final Research Report
Development of a new assay system for transcription factor activity using FCS
| Project/Area Number |
19390155
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kobe University |
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Principal Investigator |
KUMAGAI Shunichi Kobe University, 大学院・医学研究科, 教授 (00153346)
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| Co-Investigator(Kenkyū-buntansha) |
MORINOBU Akio 神戸大学, 大学院・医学研究科, 准教授 (10294216)
NISHIMURA Kunihiro 神戸大学, 大学院・医学研究科, 客員准教授 (70397834)
SAEGUSA Jun 神戸大学, 大学院・医学研究科, 特命助教 (20514970)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 自己免疫疾患 / 転写因子 / 生物製剤 / 蛍光相関分光法 |
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| Research Abstract |
First, we tried to develop a new method to measure transcription activity in clinical samples. We did so using FCS technology and ELISA technique, which showed a good correlation with each other. Second, We measured transcriptional activity in PBMC of rheumatoid arthritis patients (RA) and found that NFkB show variable titers in RA patients. In four patients receiving biologics treatment, NFkB was considered best as a marker for disease activity among NFkB, STAT3, AP-1, and NFAT. Then we checked NFkB family activity and found p50 and p65 were elevated in RA patients. Finally, we showed that p65 activity correlates with RA activity in RA patients receiving biologics therapy. Further experiments will be needed to determine whether this technique is useful to predict the efficacy of biologic therapies.
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[Journal Article] Lack of association between tyrosine kinase2(TYK2) gene polymorphisms and susceptibility to SLE in a Japanese population.2009
Author(s)
Kyogoku C, Morinobu A, Nishimura K, Sugiyama D, Hashimoto H, Tokano Y, Mimori T, Terao C, Matsuda F, Kuno T, Kumagai S.
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Journal Title
Modern Rheumatology 19(4)
Pages: 401-6
Peer Reviewed
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[Journal Article] Meta-Analysis: Diagnostic Accuracy of Anti-CCP Antibody and Rheumatoid Factor for Rheumatoid Arthritis.2007
Author(s)
Nishimura K, Sugiyama D, Kogata Y, Tsuji G, Nakazawa T, Kawano S, Saigo K, Morinobu A, Koshiba M, K.M. Kuntz, Kamae I, Kumagai S.
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Journal Title
Ann Intern Med 146
Pages: 797-813
Peer Reviewed
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