Development of rapid and simultaneous diagnosis and therapy for musculoskeletal sarcomas using acridine orange excited by high-power flash light

2009 Fiscal Year Final Research Report

• Project/Area Number: 19390390
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Mie University
• Principal Investigator: KUSUZAKI Katsuyuki Mie University, 大学院・医学研究科, 教授 (30177993)
• Co-Investigator(Kenkyū-buntansha): UCHIDA Atsumasa 三重大学, 大学院・医学研究科, 教授 (40176681) ; MATSUMINE Akihiko 三重大学, 医学部附属病院, 講師 (00335118) ; KUMAMOTO Tadashi 三重大学, 大学院・医学研究科, 助教 (50362348) ; WAKABAYASHI Hiroki 三重大学, 大学院・医学研究科, 助教 (50362687) ; SATONAKA Haruhiko 三重大学, 大学院・医学研究科, 医員 (60515485) ; NAKAMURA Tomoki 三重大学, 医学部附属病院, 医員 (50467362) ; MATSUBARA Takao 三重大学, 医学部附属病院, 医員 (30422827)
• Project Period (FY): 2007 – 2009
• Keywords: アクリジンオレンジ / 光線力学的診断 / 光線力学的治療法 / 骨軟部腫瘍 / ストロボ光

Research Abstract

To develop photodynamic diagnosis and photodynamic therapy using acridine orange excited by high-power flash light provided from xenon lamp in musculoskeletal sarcomas, we performed this study using mouse osteosarcoma model. Results showed that it was possible to brilliantly detect tumor localization through skin by PDD and to strongly inhibit tumor growth by PDT after intravenous AO injection followed by flash light illumination.

Research Products

• [Journal Article] Photodynamic therapy with acridine orangein musculoskeletal sarcomas. (2010)
  • Author(s): Matsubara T, Kusuzaki K, Matsumine A, Murata H, Marunaka Y, Hosogi S, Uchida A, Sudo A
  • Journal Title: J Bone Joint Surg (Br) (in press)
• [Journal Article] Clinical outcomes of minimally invasive surgery using acridine orange for musculoskeletal sarcomas around the forearm, compared with conventional limb salvage surgery after wide resection. (2010)
  • Author(s): Matsubara T, Kusuzaki K, Matsumine A, Murata H, Nakamura T, Uchida A, Sudo A
  • Journal Title: J Surg Oncol (in press)
• [Journal Article] In vivo anti-tumor activity of photodynamic therapy with intravenous administration of acridine orange, followed by illumination with high-power flash wave light in a mouse osteosarcoma model. (2010)
  • Author(s): Satonaka H, Kusuzaki K, Murata H, Matsubara T, Shintani K, Nakamura T, Matsumine A, Iino T, Uchida A
  • Journal Title: Oncology Letters 1 Pages: 69-72
• [Journal Article] Three-dimensional alginate spheroid culture system of murine osteosarcoma. (2009)
  • Author(s): Akeda K, Nishimura A, Satonaka H, Shintani K, Kusuzaki K, Matsumine A, Kasai Y, Masuda K, Uchida A
  • Journal Title: Oncol Rep 22 Pages: 997-1003
• [Journal Article] Decorin suppresses bone metastasis in a breast cancer cell line. (2009)
  • Author(s): Araki K, Wakabayashi H, Shintani K, Morikawa J, Matsumine A, Kusuzaki K, Sudo A, Uchida A
  • Journal Title: Oncology (Bazel) 77 Pages: 92-99
• [Journal Article] A new therapeutic modality involving acridine orange excitation by photon energy used during reduction surgery for rhabodomyosarcomas. (2009)
  • Author(s): Matsubara T, Kusuzaki K, Matsumine A, Murata H, Satonaka H, Shinntani K, Nakamura T, Hosoi H, Iehara T, Sugimoto T, Uchida A
  • Journal Title: Oncol Rep 21 Pages: 89-94
• [Journal Article] Usefulness of limb salvage surgery for bone and soft tissue sarcomas of the distal lower leg. (2008)
  • Author(s): Niimi R, Matsumine A, Kusuzaki K, Araki N, Aoki Y, Ueda T, Kudahara I, Myoui A, Ieguchi M, Hashimoto N, Yoshikawa H, Uchida A
  • Journal Title: J Cancer Res Clin Oncol 134 Pages: 1087-1095
• [Journal Article] Decorin suppresses lung metastasis of murine osteosarcoma. (2008)
  • Author(s): Shintani K, Matsumine A, Kusuzaki K, Morikawa J, Matsubara T, Wakabayashi T, Araki K, Satonaka H, Wakabayashi H, Iino T, Uchida A
  • Journal Title: Oncol Rep 19 Pages: 1533-1539
• [Journal Article] A new limb salvage surgery in cases of high-grade soft tissue sarcoma using photodynamic surgery, followed by photo-and radiodynamic therapy with acridine orange. (2008)
  • Author(s): Nakamura T, Kusuzaki K, Matsubara T, Matsumine A, Murata H, Uchida A
  • Journal Title: J Surg Oncol 97 Pages: 523-528
• [Journal Article] Novel hyperthermia for metastatic bone tumors magnetic materials by generating electromagnetic field. (2007)
  • Author(s): Matsumine A, Kusuzaki K, Matsubara T, Shintani K, Satonaka H, Wakabayashi T, Miyazaki S, Morita K, Takegami K, Uchida A
  • Journal Title: Clin Exp Metastasis 24 Pages: 191-200
• [Journal Article] Flash wave light strongly enhanced cytocidal effect of photodynamic therapy with acridine orange on mouse osteosarcoma cell line. (2007)
  • Author(s): Satonaka H, Kusuzaki K, Murata H, Matsubara T, Shintani K, Wakabayashi T, Matsumine A, Kimura M, Kawashima N, Uchida A
  • Journal Title: Anticancer Res 27 Pages: 3339-3344
• [Journal Article] Acridine orange could be an innovative anticancer agent under photon energy. (2007)
  • Author(s): Kusuzaki K, Murata H, Matsubara T, Satonaka H, Wakabayashi T, Matsumine A, Uchida A
  • Journal Title: In vivo 21 Pages: 205-214
• [Journal Article] Extracorporeal photodynamic image detection of mouse osteosarcoma in soft tissues utilizing fluorovisualization effect of acridine orange. (2007)
  • Author(s): Satonaka H, Kusuzaki K, Shintani K, Matsumine A, Matsubara T, Wakabayashi T, Uchida A
  • Journal Title: Oncology (Bazel) 70 Pages: 465-473
• [Journal Article] Methylene blue in place of acridine orange as a photosensitizer in photodynamic therapy of osteosarcoma.
  • Author(s): Matsubara T, Kusuzaki K, Matsumine A, Satonaka H, Shinntani K, Nakamura T, Uchida A
  • Journal Title: In vivo 22 Pages: 297-304
• [Presentation] In vivo antitumor activity of photodynamic therapy with intravenous administration of acridine orange followed by illumination with high-power flash wave light in a mouse osteosarcoma model. (2010)
  • Author(s): Satonaka H, Kusuzaki K, Matsubara T, Shintani K, Nakamura T, Matsumine A, Iino T, Uchida A
  • Organizer: 56th Annual Meeting of Orthopaedic Research Society. 2010 March
  • Place of Presentation: New Oreans, USA
  • Year and Date: 20100311-20100314
• [Presentation] Clinical Outcomes with the KYOCERA Physio Hinge Total Knee System Type III after the Resection of a bone and soft tissue tumor of the Distal Part of the Femur: from the Japanese Musculoskeletal Oncology Group 8th. (2010)
  • Author(s): Matsumine A, Kusuzaki K, Sugita T, Yazawa Y, Isu K, Kawai A, Abe S, Yakushiji T, Ueda T, Uchida A
  • Organizer: Asia Pacific Musculoskeletal Tumor Society Meeting
  • Place of Presentation: Sebu Philippin
  • Year and Date: 20100000
• [Presentation] Expression of SAP97 as a biomarker of outcome in malignant fibrous histiocytomas. (2009)
  • Author(s): Niimi R, Matsumine A, 1Iino T, Shintani K, Nakazora S, Nakamura T, Kusuzaki K, Uchida A
  • Organizer: 55th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Las Vegas USA
  • Year and Date: 20090308-20090310
• [Presentation] Three-dimensional alginate spheroid culture system of murine osteosarcoma. (2009)
  • Author(s): Nishimura A, Akeda K, Kusuzaki K, Matsumine A, Masuda K, Morimoto R, Okamura N, Kasai Y, 1Iino T, Uchida A
  • Organizer: 55th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Las Vegas USA
  • Year and Date: 20090308-20090310
• [Presentation] Acridine orange found in Ehrlich's era could be a "Magic Bullet" against cancer under photon energy. (2008)
  • Author(s): Kusuzaki K, Murata H, Matsubara T, Satonaka H, Nakamura T, Matsumine A, Uchida A
  • Organizer: The 2nd World Confererence on Magic Bullets (Ehrlich II)
  • Place of Presentation: Neurmberg, Germany
  • Year and Date: 20081003-20081005
• [Presentation] Novel hyperthermia for metastatic bone tumors with magnetic materials. (2008)
  • Author(s): Matsumine A, Kusuzaki K, Matsubara T, Morita K, Takegami K, Uchida
  • Organizer: 7th Asia Pacific Musculoskeletal Tumor Society Meeting
  • Place of Presentation: Beijin, China
  • Year and Date: 20080926-20080928
• [Presentation] new limb salvage surgery in cases of high-grade soft tissue sarcoma using photodynamic surgery, followed by photo- and radio dynamic therapy with acridine orange. (2008)
  • Author(s): Nakamura T, Matsumine A, Kusuzaki K, Matsubara T, Niimi R, Nakazora Murata H, S, Uchida A
  • Organizer: 7th Asia Pacific Musculoskeletal Tumor Society Meeting
  • Place of Presentation: Beijin, China
  • Year and Date: 20080926-20080928
• [Presentation] In vivo antitumor effect of photodynamic therapy with acridine orange using flash wave light on osteosarcoma. (2008)
  • Author(s): Satonaka H, Kusuzaki K, Matsubara T, Shintani K, Wakabayashi T, Nakamura T, Matsumine A, Uchida A
  • Organizer: 7th Asia Pacific Musculoskeletal Tumor Society Meeting
  • Place of Presentation: Beijin, China
  • Year and Date: 20080926-20080928
• [Presentation] Extracorporeal photodynamic image detection of mouse osteosarcoma in soft tissues utilizing fluorovisualization effect of acridine orange. (2007)
  • Author(s): Satonaka H, Kusuzaki K, Matsubara T, Shintani K, Wakabayashi T, Matsumine A, Uchida A
  • Organizer: The 14th International Symposium on Limb Salvage
  • Place of Presentation: Hamburg, Germany
  • Year and Date: 20070911-20070914
• [Presentation] Novel hyperthermia for metastatic bone tumors with magnetic materials by generating an alternating electromagnetic field. (2007)
  • Author(s): Matsumine A, Kusuzaki K, Matsubara T, Morita K, Takegami K, Uchida
  • Organizer: The 14th International Symposium on Limb Salvage
  • Place of Presentation: Hamburg, Germany
  • Year and Date: 20070911-20070914
• [Presentation] Acridine orange could be an innovative anticancer agent under photon energy. (2007)
  • Author(s): Kusuzaki K, Matsubara T, Satonaka H, Wakabayashi T, Matsumine A, Uchida A
  • Organizer: The 14th International Symposium on Limb Salvage
  • Place of Presentation: Hamburg, Germany
  • Year and Date: 20070911-20070914