Effects of fatty acids on differentiation of neuronal stem cells and the protective and ameliorative effects on dementia.

2009 Fiscal Year Final Research Report

• Project/Area Number: 19500324
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Shimane University
• Principal Investigator: HASHIMOTO Michio Shimane University, 医学部, 准教授 (70112133)
• Co-Investigator(Kenkyū-buntansha): KATAKURA Masanori 島根大学, 医学部, 助教 (40383179)
• Co-Investigator(Renkei-kenkyūsha): YAMASHITA Kazuya 島根県立大学, 短期大学部, 教授 (30210412)
• Project Period (FY): 2007 – 2009
• Keywords: 精神・神経疾患の病態と治療 / 神経幹細胞 / n-3系多価不飽和脂肪酸 / 神経新生 / ヒト介入試験

Research Abstract

1) Docosahexaenoic acid (DHA) and eicosapentaenoic acid, n-3 polyunsaturated fatty acids, promoted neurogenesis of neuronal stem cells (NSCs) isolated from rat embryos and also adult hippocampus. These n-3 fatty acids induce differentiation by causing cell cycle arrest in NSCs.
2) In randomized, double-blind and placebo-controlled trail, preventive effect of DHA supplement for 12 months on cognitive decline rate in elderly people with very mild dementia was observed.

Research Products

• [Journal Article] Docosahexaenoic acid withstands the Aβ25-35-induced neurotoxicity in SH-S5Y5 cells (2010)
  • Author(s): Michio Hashimoto, Masanori Katakura, Shahdat Hossain, Azizur Rahman, Toshio Shimada, Osamu Shido
  • Journal Title: Journal of Nutrition Biochemistry (in press)
  • Peer Reviewed
• [Journal Article] Effects of docosahexaenoic acid on in vitro amyloid βpeptide 25-35 fibrillation. (2009)
  • Author(s): Michio Hashimoto, Hossain Md Shahdat, Masanori Katakura, YokoTanabe, Shuji Gamoh, Koji Miwa, Toshio Shimada, Osamu Shido.
  • Journal Title: Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids. 1791 Pages: 289-296
  • Peer Reviewed
• [Journal Article] Docosahexaenoic acid promotes neuronal differentiation by regulating basic helix-loop-helix transcription factors and cell cycle in neural stem cells (2009)
  • Author(s): Masanori Katakura, Michio Hashimoto, Hossain Md Shahdat, Shuji Gamoh, Toshiyuki Okui, Kentaro Matsuzaki, Osamu Shido
  • Journal Title: Neuroscience 160 Pages: 651-60
  • Peer Reviewed
• [Journal Article] Mechanism of docosahexaenoic acid-induced inhibition of in vitro amyloid β1-42 fibrillation and amyloid β1-42-induced toxicity in SH-S5Y5 cells. (2009)
  • Author(s): Shahdat Hossain, Michio Hashimoto, Masanori Katakura, Koji Miwa, Toshio Shimada, Osamu Shido.
  • Journal Title: Journal of Neurochemistry 111 Pages: 568-579
  • Peer Reviewed
• [Journal Article] The protective effect of dietary eicosapentaenoic acid on the impairment of spatial cognition learning ability in rats infused with amyloid β(1-40). (2009)
  • Author(s): Michio Hashimoto, Shahdat Hossain, Yoko Tanabe, Akiko Kawashima, Tsuyoshi Harada, Takashi Yano, Kiyoshi Mizuguchi, Osamu Shido.
  • Journal Title: Journal of Nutritional Biochemistry 20 Pages: 965-973
  • Peer Reviewed
• [Journal Article] 多価不飽和脂肪酸と疾患2中枢神経系 (2009)
  • Author(s): 橋本道男
  • Journal Title: 治療学 43 Pages: 838-844
  • Peer Reviewed
• [Journal Article] DHA disrupts in vitro amyloid β1-40 fibrillation and concomitantly inhibits amyloid levels in cerebral cortex tissue of Alzheimer's disease model rats. (2008)
  • Author(s): Michio Hashimoto, Hossain Md Shahdat, Shinji Yamashita, Masanori Katakura, Yoko Tanabe, Hironori Fujiwara, Shuji Gamoh, Teruo Miyazawa, Noriyuki Arai, Toshio Shimada, Osamu Shido.
  • Journal Title: Journal of Neurochemistry 107 Pages: 1634-1646
  • Peer Reviewed
• [Journal Article] 食事で防ぐ認知症 (2008)
  • Author(s): 橋本道男、山下一也
  • Journal Title: 最新精神医学 13 Pages: 75-82
  • Peer Reviewed
• [Journal Article] ω3多価不飽和脂肪酸と中枢神経疾患 (2008)
  • Author(s): 橋本道男、蒲生修治、片倉賢紀、Hossain Shahdat
  • Journal Title: The Lipid 19(4) Pages: 409-419
  • Peer Reviewed
• [Journal Article] アルツハイマー病と食事栄養-とくに銅・亜鉛とドコサヘキサエン酸 (2008)
  • Author(s): 橋本道男
  • Journal Title: Trace Nutrients Research 25 Pages: 8-18
  • Peer Reviewed
• [Presentation] n-3系脂肪酸によるアルツハイマー型認知症予防・改善効果 (2010)
  • Author(s): 橋本道男、ホサイン・シャハダト、片倉賢紀
  • Organizer: 第83回日本薬理学会年会シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-17
• [Presentation] ドコサヘキサエン酸(dha)と認知・記憶機能-認知症への介入試験の科学的根拠と作用メカニズム (2010)
  • Author(s): 橋本道男、ホサイン・シャハダト、片倉賢紀
  • Organizer: 第13回日本病態栄養学会年次学術集会ワークショップ
  • Place of Presentation: 京都
  • Year and Date: 2010-01-10
• [Presentation] Effect of polyunsaturated fatty acids on the cell cycle and the expression of bHLH transcriptional factors in neural stem cells. (2009)
  • Author(s): Masanori Katakura, Michio Hashimoto, Shuji Gamoh, Toshiyuki Okui, Kentaro Matsuzaki, Osamu Shido.
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto
  • Year and Date: 20090727-20090801
• [Presentation] Docosahexaenoic acid improves behavioral impairment in amyloidβ(Aβ)-infused rats, by decreasing both in vitro and Aβlevels in the brain. (2009)
  • Author(s): Michio Hashimoto, Hossain Md Shahdat, Masanori Katakura, Yoko Tanabe, Toshio Shimada, Osamu Shido.
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20090727-20090801
• [Presentation] Inhibiting effect of docosahexaenoic acid on the amyloid beta peptide fibrillation. (2009)
  • Author(s): Michio Hashimoto, Hossain Md. Shahdat, Masanori Katakura, Osamu Shido.
  • Organizer: 2009 International Conference on Alzheimer's Disease (ICAD)
  • Place of Presentation: Vienna, Austria
  • Year and Date: 20090711-20090716
• [Presentation] Docosahexaenoic acid attenuates in vitro Aβfibrillation with concomitant decreases of Aβpeptide levels in brain of AD model rats. (2009)
  • Author(s): Michio Hashimoto, Hossain Md Shahdat, Masanori Katakura, Yoko Tanabe, Shuji Gamoh, Osamu Shido.
  • Organizer: 9th International Conference AD/PD 2009
  • Place of Presentation: Praque, Czech Republic
  • Year and Date: 20090311-20090315
• [Presentation] 脳機能賦活物質としてのドコサヘキサエン酸の魅力―島根疫学研究と基礎実験データから (2009)
  • Author(s): 橋本道男
  • Organizer: 第8回ホスファチジルセリン研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-20
• [Presentation] DHAによる脳・神経機能の維持 (2009)
  • Author(s): 橋本道男
  • Organizer: DHA・EPA協議会主催第11回公開講演会「DHA・EPAによる疾病予防」
  • Place of Presentation: 東京
  • Year and Date: 2009-10-27
• [Presentation] Docosahexaenoic acid controls the expression of Hes1 and p27 on the neural stem cell differentiation神経幹細胞におけるHes1とp27発現量のドコサヘキサエン酸による調節 (2009)
  • Author(s): Masanori Katakura, Michio Hashimoto, Hossain M Shahdat, Shuji Gamoh, Toshiyuki Okui, Osamu Shido
  • Organizer: 第32回日本神経科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] 神経幹細胞のニューロン分化に及ぼす9-cis, 11-trans-共役リノール酸の影響 (2009)
  • Author(s): 奥井俊之、橋本道男、片倉賢紀、紫藤治
  • Organizer: 日本脂質栄養学会第18回大会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-04
• [Presentation] 神経幹細胞のニューロンへの分化にともなうHes1とp27の発現量の変化に及ぼすドコサヘキサエン酸の影響 (2009)
  • Author(s): 片倉賢紀、橋本道男、奥井俊之、紫藤治
  • Organizer: 日本脂質栄養学会第18回大会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-04
• [Presentation] 食事で防ぐ認知症 (2008)
  • Author(s): 橋本道男
  • Organizer: 日本食品保蔵科学会第57大会市民セミナー
  • Place of Presentation: 松江市
  • Year and Date: 20080621-20080622
• [Presentation] 多価不飽和脂肪酸による神経幹細胞の増殖・分化の変化 (2008)
  • Author(s): 片倉賢紀、橋本道男、蒲生修治、奥井俊之、紫藤治
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 20080325-20080327
• [Presentation] ドコサヘキサエン酸(DHA)と脳機能-基礎研究から臨床応用に向けて (2008)
  • Author(s): 橋本道男
  • Organizer: 日本脂質栄養学会第17回大会サテライトシンポジウム「脳機能・行動・神経症に影響を与える脂質栄養」
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-07
• [Presentation] The role of docosahexaenoic acid for the constitution, function and maintenance of central nervous system. (2008)
  • Author(s): Shahdat Hossain, Shuji Gamoh, Masanori Katakura, Michio Hashimoto.
  • Organizer: 第31回日本神経科学会大会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] 魚油成分(DHA・EPA)による認知症予防・改善効果 (2008)
  • Author(s): 橋本道男
  • Organizer: 第25回日本微量栄養素学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-05-30
• [Presentation] 認知症と食事栄養 (2008)
  • Author(s): 橋本道男
  • Organizer: 第3回山陰高齢医学研究会
  • Place of Presentation: 松江
  • Year and Date: 2008-04-25
• [Presentation] DHAによる認知症の改善 (2007)
  • Author(s): 橋本道男
  • Organizer: 第8回全国エゴマサミット
  • Place of Presentation: 庄原
  • Year and Date: 20070523-20070524
• [Presentation] FISH OIL CONSUMPTION AND COGNITIVE FUNCTION IN ELDERLY PEOPLE: FROM SHIMANE COHORT STUSY IN JAPAN. (2007)
  • Author(s): Michio Hashimoto, Kazuya Yamashita, Masanori Katakura, Yuri Iyama, Ichie Matsumoto, Ayako Matsuoka, Osamu Shido, Setsushi Kato, Akira Ueki.
  • Organizer: 8th International Conference AD/PD 2007
  • Place of Presentation: Salzburg, Austria
  • Year and Date: 20070314-20070318
• [Presentation] MECHANISMS OF DIETARY DOCOSAHEXAENOIC ACID-INDUCED PROTECTION AND AMELIORATION ON IMPAIRMENT OF MEMORY LEARNING IN ALZHEIMER'S DISEASE MODEL RATS. (2007)
  • Author(s): Michio Hashimoto, Hossain Md Shahdat, Shinji Yamashita, Masanori Katakura, Yoko Tanabe, Hironori Fujiwara, Shuji Gamoh, Teruo Miyazawa, Noriyuki Arai, Osamu Shido.
  • Organizer: 8th International Conference AD/PD 2007
  • Place of Presentation: Salzburg, Austria
  • Year and Date: 20070314-20070318
• [Presentation] PROMOTION OF NEUROGENESIS BY DOCOSAHEXAENOIC ACID IN VIVO AND IN VITRO. (2007)
  • Author(s): Michio Hashimoto, Masanori Katakura, Eisuke Kawakita, Toshiyuki Okui, Shuji Gamoh, Haque Md Abdul, Budbazar Enkhjargal, Osamu Shido.
  • Organizer: 8th International Conference AD/PD 2007
  • Place of Presentation: Salzburg, Austria
  • Year and Date: 20070314-20070318
• [Presentation] n-3系脂肪酸、特にドコサヘキサエン酸による脳機能改善作用と認知症予防・改善効果 (2007)
  • Author(s): 橋本道男
  • Organizer: 日本脂質栄養学会第16回大会シンポジウム
  • Place of Presentation: 出雲市
  • Year and Date: 2007-08-31
• [Presentation] n-3系脂肪酸と認知症 (2007)
  • Author(s): 橋本道男、片倉賢紀、田邊洋子、ホサイン・シャハダト、蒲生修治、宮澤陽夫、荒井啓行、紫藤治
  • Organizer: 第80回日本薬理学会年会シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2007-03-05
• [Book] Current opinions regarding fish oils remedies in the near future( E. P. Heikkinen) (2008)
  • Author(s): Shuji Gamoh, Shahdat Md Hossain, Michio Hashimoto
  • Total Pages: 87-115
  • Publisher: Nova Science Publishers, Inc., New York
• [Remarks] DHA強化ソーセージによるヒト試験に関するインターネット・新聞報道
• [Remarks] 中国新聞2010年3月1日
• [Remarks] 水産タイムス2010年2月4日
• [Remarks] 日刊水産通信2010年2月4日
• [Remarks] 山陰中央新報2010年2月3日朝刊
• [Remarks] みなと新聞2010年2月3日
• [Remarks] 毎日新聞2010年2月3日夕刊第2面、朝刊第3面
• [Remarks] ヤフートップニュース2010年2月3日青魚と認知症予防についてNHK総合テレビ2010年3月29日「ゆうどきネットワーク」(16:55～18:00、全国放送)で放映