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2008 Fiscal Year Final Research Report

Functional analysis of reducing equivalents transfer system on the thylakoid membranes

Research Project

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Project/Area Number 19570033
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionTokyo Institute of Technology

Principal Investigator

MOTOHASHI Takeshi  Tokyo Institute of Technology, 資源化学研究所, 資源化学研究所特別研究員 (90301952)

Project Period (FY) 2007 – 2008
Keywordsオルガネラ / 葉緑体 / 酸化還元制御
Research Abstract

チラコイド膜を介した還元力供給過程に関連すると考えられるシステムを明らかにするため、本研究ではこれに関連することが予想されるタンパク質の抗体作成およびそれらの抗体を用いたin vitroアッセイ系の構築を目指してin vitroでの還元力伝達アッセイ実験を行った。その結果、関連することが予想されるタンパク質の抗体を用いてin vitro還元力伝達アッセイ系を行った結果、チラコイド膜を介した還元力の伝達に膜因子が関与する可能性が示唆された。

  • Research Products

    (9 results)

All 2009 2008 2007

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (4 results)

  • [Journal Article] Identification of thioredoxin targeted proteins using thioredoxin single cysteine mutant-immobilized resin Methods in Molecular Biology2009

    • Author(s)
      Ken Motohashi, Patrick G. N. Romano and Toru Hisabori
    • Journal Title

      479

      Pages: 117-131

    • Peer Reviewed
  • [Journal Article] Toru Hisabori and Tatsuru Masuda Functional analysis of Arabidpsis thaliana isoforms of the Mg-chelatase CHLI subunit2008

    • Author(s)
      Koichi Kobayashi, Nobuyoshi Mochizuki, Naho Yoshimura, Ken Motohashi
    • Journal Title

      Photochemical & Photobiological Sciences7

      Pages: 1188-1195

    • Peer Reviewed
  • [Journal Article] Binary reducing equivalent pathways using NADPH-thioredoxin reductase and ferredoxin-thioredoxin reductase in the cyanobacterium Synechocystis sp2008

    • Author(s)
      Shoko Hishiya, Wakako Hatakeyama, Yoko Mizota, Naomi Hosoya-Matsuda, Ken Motohashi, Masahiko Ikeuchi and Toru Hisabori
    • Journal Title

      strain PCC 6803 Plant and Cell Physiology 49

      Pages: 11-18

    • Peer Reviewed
  • [Journal Article] The significance of type II and PrxQ peroxiredoxins for antioxidative stress response in the purple bacterium rhodobacter sphaeroides2007

    • Author(s)
      Masahiro Wakita, Shinji Masuda, Ken Motohashi, Toru Hisabori, Hiroyuki Ohta and Ken-ichiro Takamiya
    • Journal Title

      The Journal of Biological Chemistry 282

      Pages: 27792-27801

    • Peer Reviewed
  • [Journal Article] The CHLI1 subunit of Arabidopsis thaliana magnesium chelatase is a target protein of the chloroplast thioredoxin2007

    • Author(s)
      Akinori Ikegami, Naho Yoshimura, Ken Motohashi, Shigekazu Takahashi, Patrick G. N. Romano, Toru Hisabori, Ken-ichiro Takamiya and Tatsuru Masuda
    • Journal Title

      The Journal of Biological Chemistry 282

      Pages: 19282-19291

    • Peer Reviewed
  • [Presentation] 葉緑体チラコイド膜を介したストロマから内腔への還元力伝達機構の解明2009

    • Author(s)
      本橋健、久堀徹
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学東山キャンパス
    • Year and Date
      20090321-24
  • [Presentation] Redox cascade through thioredoxin-family proteins in plant chloroplast2008

    • Author(s)
      Ken MOTOHASHI, Toru HISABORI
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会(BMB2008)
    • Place of Presentation
      神戸・ポートピア
    • Year and Date
      20081209-12
  • [Presentation] Physiological significance of the thioredoxin networks in chloroplasts2007

    • Author(s)
      久堀徹、本橋健、原怜、丸井弘嗣、池上陽紀、吉村奈穂、増田建
    • Organizer
      第30回日本分子生物学会、第80回日本生化学会合同大会(BMB2007)
    • Place of Presentation
      横浜, パシフィコ横浜
    • Year and Date
      20071211-15
  • [Presentation] Functional analysis of the luminal thioredoxin-like protein, HCF1642007

    • Author(s)
      Ken MOTOHASHI, Masasuke YOSHIDA, Toru HISABORI
    • Organizer
      The 14th International Congress of Photosynthesis
    • Place of Presentation
      Glasgow, United Kingdom
    • Year and Date
      20070722-27

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Published: 2010-06-10   Modified: 2016-04-21  

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