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2009 Fiscal Year Final Research Report

GCNF mediated DNA methylation and stem cell differentiation

Research Project

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Project/Area Number 19570146
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SATO Noriko  Tokyo Medical and Dental University, 難治疾患研究所, 准教授 (70280956)

Project Period (FY) 2007 – 2009
KeywordsDNAメチル化 / エピジェネティック制御
Research Abstract

GCNF (germ cell nuclear factor) is expressed at the gastrulation stage during embryonic development. GCNF represses pluripotency genes such as Oct-3/4 and Nanog and induces DNA methylation of their regulatory regions. In this project, the specific monoclonal antibodies against GCNF have been newly prepared in order to analyze the endogenous GCNF protein during murine ES cell differentiation. By the co-immunoprecipitation study, interaction between endogenous GCNF and Dnmt3 proteins was confirmed. At the beginning of ES differentiation, the expression level of GCNF was low, but it interacts with Dnmt3a2 and Dnmt3b. At the later stage, GCNF was transiently upregulated and it interacts with Dnmt3a1. Mass spectrometry analysis has revealed that anti-GCNF immunoprecipitates contain Trim28 and PELP1 complex, but the further analyses will be required to assess its functional significance.

  • Research Products

    (1 results)

All 2007

All Presentation (1 results)

  • [Presentation] 核内受容体によるDNAメチル化誘導機構2007

    • Author(s)
      佐藤憲子, 新井賢一
    • Organizer
      第1回日本エピジェネティクス研究会年会
    • Year and Date
      2007-06-15

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Published: 2011-06-18   Modified: 2016-04-21  

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