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2009 Fiscal Year Final Research Report

Isolation of the high Al tolerant genes derived from wild plants and application of these genes to heavy metal tolerance in plant

Research Project

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Project/Area Number 19580066
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionOkayama University

Principal Investigator

EZAKI Bunichi  Okayama University, 資源生物科学研究所, 准教授 (90243500)

Co-Investigator(Kenkyū-buntansha) NAKASHIMA Susumu  岡山大学, 資源生物科学研究所, 准教授 (60033122)
Project Period (FY) 2007 – 2009
Keywords野生植物 / 多種ストレス耐性機構 / 重金属ストレス / Alストレス / 酸化ストレス / ABC transporter protein / S-adenosyl methionine sythetase (SAMS) / 耐性遺伝子
Research Abstract

The aluminum (Al) tolerant mechanism in the two wild plants, Andropogon virginicus L and Miscanthus sinensis, were investigated. ABC transporter gene was isolated as an Al inducible genes from Andropogon. The gene was also response to oxidative stress and heavy metals. Other Andropogon genes which can confer multiple tolerance to various stresses were isolated. One of them was S-adenosyl methionine sysnthetase gene. Finally, we could isolate full length cDNA encoding these two genes from Andropogon.

  • Research Products

    (16 results)

All 2010 2009 2008 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (9 results) Remarks (1 results)

  • [Journal Article] A phage display combined with DNA affinity magnetic system can be applied to a screening of DNA binding proteins, such as transcription factors2010

    • Author(s)
      Kusumadewi Sri Yulita, Takafumi Kouno, Bunichi Ezaki
    • Journal Title

      Electronic Journal of Biotechnology 13

    • Peer Reviewed
  • [Journal Article] Aluminum tolerance associated with enhancement of plasma membrane H+-ATPase in the root apex of soybean2010

    • Author(s)
      Y. Kim, W. Park, H. Nian, T. Sasaki, B. Ezaki,, Y. Jang, G. Chung, H. Bae, S. Ahn
    • Journal Title

      Soil Science and Plant Nutrition 56

      Pages: 140-149

    • Peer Reviewed
  • [Journal Article] Role of N-terminal His-rich domain of Oscillatoria brevis Bxa1 in both Ag(I)/Cu(I) and Cd(II)/Zn(II) tolerance.2009

    • Author(s)
      E. Nakakihara, H. Kondo H, S. Nakashima, B. Ezaki
    • Journal Title

      The Open Microbiology Journal 3

      Pages: 15-22

    • Peer Reviewed
  • [Journal Article] Gold induces a metallothionein gene and a CPx-ATPase gene through regulation of a repressor BxmR in Oscillatoria brevis.2009

    • Author(s)
      S. Tagawa, S. Nakashima, B. Ezaki
    • Journal Title

      Japanese Journal of Water Treatment Biology 45

      Pages: 107-113

    • Peer Reviewed
  • [Journal Article] Over-expression of an auxilin-like gene (F9E10. 5) can suppress Al uptake in roots of Arabidopsis.2008

    • Author(s)
      B. Ezaki, H. Kiyohara, H. Matsumoto, S. Nakashima
    • Journal Title

      58

      Pages: 497-506

    • Peer Reviewed
  • [Journal Article] Screening of high-tolerant wild plants simultaneously to aluminum, heavy metal stresses and oxidative stresses, and characterization of their unique tolerance mechanisms.2008

    • Author(s)
      B. Ezaki, E. Nagao, Y. Yamamoto, S. Nakashima, T. Enomoto
    • Journal Title

      Plant Cell Reports 27

      Pages: 951-961

    • Peer Reviewed
  • [Presentation] 野生植物Andropogon virginicus L.のAlストレスに応答するABC transporter様遺伝子とSAMS遺伝子の解析2010

    • Author(s)
      高橋憲公, 東藍子, 江崎文一
    • Organizer
      日本植物生理学会
    • Place of Presentation
      熊本
    • Year and Date
      20100318-20100321
  • [Presentation] Aluminum and heavy metals stimulate nitric oxide (NO) production in Andropogon virginicus L.2010

    • Author(s)
      Jayaram K, Ezaki B
    • Organizer
      日本植物生理学会
    • Place of Presentation
      熊本
    • Year and Date
      20100318-20100321
  • [Presentation] 多種のストレスに応答するAtGST11遺伝子の応答に関わる転写調節因子の単離と解析2010

    • Author(s)
      河野貴文, 江崎文一
    • Organizer
      日本植物生理学会
    • Place of Presentation
      熊本
    • Year and Date
      20100318-20100321
  • [Presentation] 重金属ストレスに対するラン藻Oscillatoria brevisのリプレッサー蛋白BxmRの機能に関する分子遺伝学的解析2009

    • Author(s)
      田川, 中島, 江崎
    • Organizer
      日本植物生理学会
    • Place of Presentation
      名古屋
    • Year and Date
      20090321-20090324
  • [Presentation] 野生植物メリケンカルカヤ(Andropogon virginicus L. )の持つ金属ストレス及び酸化ストレス耐性遺伝子群の単離と解析2009

    • Author(s)
      高橋, 河野, 東, 中島, 江崎
    • Organizer
      日本植物生理学会
    • Place of Presentation
      名古屋
    • Year and Date
      20090321-20090324
  • [Presentation] 金属ストレスや酸化ストレス等の多種のストレスに応答するアラビドプシスのAtGST11遺伝子の応答に関わる転写調節因子の単離と解析2009

    • Author(s)
      河野, Kusumadewi. S. Yulita, 中島, 江崎
    • Organizer
      日本植物生理学会
    • Place of Presentation
      名古屋
    • Year and Date
      20090321-20090324
  • [Presentation] Wild plants including Andropogon virginicus L and Miscanthus sinensis show high tolerance to aluminum heavy metal stresses and oxidative stress by combination of their tolerance mechanism2008

    • Author(s)
      B. Ezaki
    • Place of Presentation
      Banasthali, India
    • Year and Date
      20081019-20081021
  • [Presentation] 野生植物メリケンカルカヤやススキ由来の金属ストレスや酸化ストレス耐性機構および応答機構に関する解析2008

    • Author(s)
      江崎, 高橋, Metwally, 河野, 中木原, 東, 榎本, 中島
    • Organizer
      日本肥料学会
    • Place of Presentation
      名古屋
    • Year and Date
      20080909-20080913
  • [Presentation] Molecular genetic characterization for tolerant mechanism and response mechanism against Al stress in plants.2008

    • Author(s)
      B. Ezaki
    • Organizer
      Globalization of Human Resources
    • Place of Presentation
      Gwanjyu, Korea
    • Year and Date
      2008-11-20
  • [Remarks] 代表者の所属する研究グループのホームページに研究内容に関する記載がある

    • URL

      http://www.rib.okayama-u.ac.jp/sentan/indexj.html

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Published: 2011-06-18   Modified: 2016-04-21  

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