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2009 Fiscal Year Final Research Report

Development of transformation system in Lombardy poplar by using a poplar gene as a selective marker gene

Research Project

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Project/Area Number 19580178
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林学・森林工学
Research InstitutionForestry and Forest Products Research Institute

Principal Investigator

NISHIGUCHI Mitsuru  Forestry and Forest Products Research Institute, 生物工学研究領域, 主任研究員 (80353796)

Project Period (FY) 2007 – 2009
Keywordsポプラ / 遺伝子組換え / 選抜マーカー遺伝子 / ツニカマイシン / GPT / バイナリーベクター / 遺伝子発現 / 出芽酵母
Research Abstract

To obtain a selective marker gene for generation of transgenic trees, we cloned and sequenced the cDNA of UDP-N-acetylglucosamine : dolichyl-phosphate N-acetylglucosaminephosphotransferase (GPT) from Lombardy poplar (Populus nigra var. italica). Overexpression of GPT in budding yeast conferred tunicamycin tolerance. We succeeded in the generation of GPT-introduced transgenic poplars by using tunicamycin and a new binary vector, which contained the cDNA of GPT as a selective marker gene.

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Published: 2011-06-17   Modified: 2016-04-21  

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