2009 Fiscal Year Final Research Report
Discrimination method for Takifugu hybrids using AFLP and mtDNA analyses
| Project/Area Number |
19580229
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | National Fisheries University |
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Principal Investigator |
SAKAI Harumi National Fisheries University, 生物生産学科, 教授 (80399659)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Hiroshi 独立行政法人水産大学校, 生物生産学科, 助教 (90399650)
MATSUURA Keiichi 独立行政法人国立科学博物館, コレクションディレクター (70141984)
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| Research Collaborator |
NISHIDA Mutsumi 東京大学, 海洋研究所, 所長 (90136896)
YAMANOUE Yusuke 東京大学, 海洋研究所 (90572107)
DOI Hiroyuki 下関市立水族館, 海響館
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| Project Period (FY) |
2007 – 2009
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| Keywords | 分類学 / 雑種 / トラフグ属 / mtDNA / AFLP |
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| Research Abstract |
Phylogenetic relationships of tetraodontiform fishes within teleosts, of tetraodontiform fishes, and of Takifugu species were clarified using mtDNA full sequences analysis. Hybrid analysis was also performed among 9 Takifugu species using AFLP method. All species other than between T.rubripes and T.chinensis were clearly distinguishable based on species specific AFLP markers. Their hybrids were also discriminated and 46 out of 47 hybrids were those of F1 generation. Developing of convenient primers for specific AFLP markers is needed in order to identify Takifugu hybrids as well as species.
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