Mechanism for control of gastrointestinal motility by a novel peptides released from the stomach.

2009 Fiscal Year Final Research Report

• Project/Area Number: 19580337
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Gifu University
• Principal Investigator: SHIMIZU Yasutake Gifu University, 応用生物科学部, 教授 (40243802)
• Project Period (FY): 2007 – 2009
• Keywords: グレリン / デスアシルグレリン / 消化管運動 / 排便中枢 / 骨盤神経 / 過敏性腸症候群 / 脊髄

Research Abstract

最近、食欲を変化させるグレリンと呼ばれるホルモンが胃から分泌されることが発見された。この研究の目的は、グレリンに消化管の運動を調節する作用があるか明らかにすることである。ラットを用いた実験から、グレリンは胃だけではなく脊髄の中でも作られていて、脊髄で作られるグレリンが自律神経を活発にして、大腸の運動を強くすることがわかった。ストレスによる下痢や便秘を改善するために応用可能な現象であると考えられる。

Research Products

• [Journal Article] Functional and in situ hybridisation evidence that preganglionic sympathetic vasoconstrictor neurons express ghrelin receptors. (2010)
  • Author(s): Ferens, D.M., Yin, L., Bron, R., Hunne, B., Ohashi-Doi, K., Kitchener, P.D., Sanger, G.J., Witherington, J., Shimizu, Y., Furness, J.B.
  • Journal Title: Neuroscience Vol.166 Pages: 671-679
  • Peer Reviewed
• [Journal Article] Contractile properties of esophageal striated muscle: comparison with cardiac and skeletal muscles in rats. (2010)
  • Author(s): Shiina, T., Shima, T., Masuda, K., Hirayama, H., Iwami, M., Takewaki, T., Kuramoto, H., Shimizu, Y.
  • Journal Title: J. Biomed. Biotech. Vol.2010 Pages: 7
  • Peer Reviewed
• [Journal Article] Contractile responses induced by physalaemin, an analogue of substance P, in the rat esophagus. (2010)
  • Author(s): Shiina, T., Shima, T., Hirayama, H., Kuramoto, H., Takewaki, T., Shimizu, Y.
  • Journal Title: Eur. J. Pharmacol. Vol.628 Pages: 202-206
  • Peer Reviewed
• [Journal Article] The neural regulation of the mammalian esophageal motility and its implication for esophageal diseases. (2010)
  • Author(s): Shiina, T., Shima, T., Worl, J., Neuhuber, W.L., Shimizu, Y.
  • Journal Title: Pathophysiology Vol.17 Pages: 129-133
  • Peer Reviewed
• [Journal Article] Oral administration of a centrally-acting ghrelin receptor agonist to conscious rats triggers defecation. (2009)
  • Author(s): Shafton, A.D., Sanger, G.J., Witherington, J., Brown, J.D., Muir, A., Butler, S., Abberley, L., Shimizu, Y., Furness, J.B.
  • Journal Title: Neurogastroenterol. Motil. Vol.21 Pages: 71-77
  • Peer Reviewed
• [Journal Article] Neuhuber WL Galanin modulates vagally induced contractions in the mouse esophagus. (2009)
  • Author(s): Boudaka A, Worl J, Shiina T, Shimizu Y, Takewaki T.
  • Journal Title: Neurogastroenterol. Motil. Vol.21 Pages: 180-188
  • Peer Reviewed
• [Journal Article] Tachykinins and their functions in the gastrointestinal tract. (2008)
  • Author(s): Shimizu, Y., Matsuyama, H., Shiina, T., Takewaki, T., Furness, J. B
  • Journal Title: Cell. Mol. Life Sci. Vol.65 Pages: 295-311
  • Peer Reviewed
• [Journal Article] Effects of NMDA receptor antagonists on visceromotor reflexes and on intestinal motility (2007)
  • Author(s): Shafton AD, Bogeski G, Kitchener P, Sanger GJ, Furness JB, Shimizu Y
  • Journal Title: in vivo. Neurogastroenterol. Motil. Vol.19 Pages: 617-624
  • Peer Reviewed
• [Journal Article] Involvement of TRPV1-dependent and-independent components in the regulation of vagally induced contractions in the mouse esophagus. (2007)
  • Author(s): Boudaka A, Worl J, Shiina T, Neuhuber WL, Kobayashi H, Shimizu Y, Takewaki T
  • Journal Title: Eur J Pharmacol. Vol.556 Pages: 157-165
  • Peer Reviewed
• [Journal Article] Impairment and restoration of spontaneous contractile activity of longitudinal smooth muscles in the TNBS-inflamed hamster distal colon. (2007)
  • Author(s): Gurung, Y.B., Shimizu, Y., Shiina, T., Mahmoud, M.E., Saito, S., Takewaki, T.
  • Journal Title: Biomed. Res. Vol.28 Pages: 301-308
  • Peer Reviewed
• [Journal Article] Key role of mucosal primary afferents in mediating the inhibitory influence of capsaicin on vagally mediated contractions in the mouse esophagus. (2007)
  • Author(s): Boudaka A, Worl J, Shiina T, Saito S, Atoji Y, Kobayashi H, Shimizu Y, Takewaki T
  • Journal Title: J Vet Med Sci. Vol.69 Pages: 365-372
  • Peer Reviewed
• [Journal Article] Purinergic control of the quail rectum: Modulation of adenosine 5'-triphosphate-mediated contrac-tion with acetylcholine. (2007)
  • Author(s): Shiina T, El-Mahmoudy A, Khalifa M, Draid M, Shimizu Y, Takewaki T
  • Journal Title: Res. Vet. Sci. Vol.82 Pages: 246-251
  • Peer Reviewed
• [Presentation] in vivoの実験系を用いた消化管運動の解析:グレリンの脊髄排便中枢を介する大腸運動促進作用 (2010)
  • Author(s): 志水泰武, 平山晴子, 嶋剛士, 椎名貴彦
  • Organizer: 第149回日本獣医学会
  • Place of Presentation: 武蔵野
  • Year and Date: 2010-03-27
• [Presentation] ラット食道横紋筋の収縮特性および内在神経系による運動調節機構 (2010)
  • Author(s): 椎名貴彦, 武脇義, 志水泰武
  • Organizer: 第149回日本獣医学会
  • Place of Presentation: 武蔵野
  • Year and Date: 2010-03-27
• [Presentation] グレリン、デスアシルグレリンおよびオベスタチンがラット大腸運動に及ぼす影響 (2010)
  • Author(s): 平山晴子, 椎名貴彦, 志水泰武
  • Organizer: 第20回病態生理学会
  • Place of Presentation: 奈良
  • Year and Date: 2010-01-23
• [Presentation] The role of ghrelin, des-acyl ghrelin and obestatin in the regulation system of colorectal motility in rats. (2009)
  • Author(s): Haruko Hirayama, Takehiko Toda, Takahiko Shiina, Hirofumi Kuramoto, Tadashi Takewaki, John B Furness, Yasutake Shimizu
  • Organizer: 2009 International Symposium on Ghrelin
  • Place of Presentation: 東京
  • Year and Date: 2009-11-19
• [Presentation] グレリンによるラット大腸運動促進効果に対するデスアシルグレリンおよびオベスタチンの拮抗作用 (2009)
  • Author(s): 平山晴子, 戸田壮彦, 椎名貴彦, 藏本博史, 志水泰武
  • Organizer: 第148回獣医学会
  • Place of Presentation: 鳥取
  • Year and Date: 2009-09-26
• [Presentation] TRPV1はマウス大腸炎に伴う消化管運動の低下に関与する (2009)
  • Author(s): 山川友佳, 椎名貴彦, 志水泰武
  • Organizer: 第148回獣医学会
  • Place of Presentation: 鳥取
  • Year and Date: 2009-09-26
• [Presentation] Antagonistic effects of des-acyl ghrelin and obestatin on ghrelin-induced enhancement of colorectal motility at the lumbo-sacral defecation center in rats. (2009)
  • Author(s): H. Hirayama, T. Toda, T. Shiina, H. Kuramoto, T. Takewaki, J.B. Furness, Y. Shimizu
  • Organizer: 国際自律神経学会
  • Place of Presentation: ISAN2009:シドニー
  • Year and Date: 2009-09-03
• [Presentation] Neuronal regulation of the esophageal motility in Suncus murinus (a house musk shrew). (2009)
  • Author(s): T. Shiina, J. Worl, W.L. Neuhuber, T. Shima, T. Takewaki, Y. Shimizu
  • Organizer: 国際自律神経学会
  • Place of Presentation: ISAN2009:シドニー
  • Year and Date: 2009-09-03
• [Presentation] Roles of a capsaicin-sensitive neural circuit in peristaltic motility of the rat esophagus. (2009)
  • Author(s): T. Shima, T. Shiina, Y. Shimizu
  • Organizer: 国際自律神経学会
  • Place of Presentation: ISAN2009:シドニー
  • Year and Date: 2009-09-03
• [Presentation] Enhancement of colorectal motility by ghrelin, but not des-acyl ghrelin, through an activation of lumbo-sacral defecation center in rats. (2009)
  • Author(s): Y Shimizu, H Hirayama, T Shiina, JB Furness, T Takewaki
  • Organizer: 国際生理学会
  • Place of Presentation: 京都
  • Year and Date: 2009-07-29
• [Presentation] Modulatory actions of tachykinins on the striated and smooth muscle motility in the rat esophagus. (2009)
  • Author(s): T Shiina, T Shima, H Hirayama, A Boudaka, J Woerl, WL Neuhuber, T Takewaki, Y Shimizu
  • Organizer: 国際生理学会
  • Place of Presentation: 京都
  • Year and Date: 2009-07-29
• [Presentation] アシル化グレリンおよびデスアシルグレリンのラット大腸運動に対する作用 (2009)
  • Author(s): 平山晴子, 椎名貴彦, 志水泰武
  • Organizer: 第51回日本平滑筋学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-22
• [Presentation] 脊髄排便中枢を介するグレリンの結直腸運動促進作用 (2008)
  • Author(s): 平山晴子, 椎名貴彦, 武脇義, 志水泰武
  • Organizer: 第55回中部日本生理学会
  • Place of Presentation: 愛知
  • Year and Date: 2008-10-17
• [Presentation] Acylated ghrelin, but not des-acyl ghrelin, enhances colorectal motility by activating lumbo-sacral defecation center in rats. (2008)
  • Author(s): Shimizu Y, Hirayama H, Shiina T, Furness JB, Takewaki T
  • Organizer: 第81回薬理学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-02-18
• [Presentation] グレリンの排便中枢を介する結直腸運動亢進作用 (2008)
  • Author(s): 志水泰武, 椎名貴彦
  • Organizer: 第18回病態生理学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-01-26
• [Presentation] Evidence that activation of ghrelin receptors of autonomic preganglionic neurons stimulates autonomic outflows. (2007)
  • Author(s): Shimizu, Y
  • Organizer: 国際自律神経学会
  • Place of Presentation: ISAN2007:京都
  • Year and Date: 2007-10-07
• [Presentation] Evidence that activation of ghrelin receptors of preganglionic neurons in the spinal cord stimulates colorectal motility and defecation (2007)
  • Author(s): Y. Shimizu, A.D. Shafton, D.M. Ferens, B. Hunne, J.B. Furness
  • Organizer: 国際シンポジウム「Gastrointestinal motility and Functional gastrointestinal disorders, Basic to Clinical」
  • Place of Presentation: 名古屋
  • Year and Date: 2007-08-28
• [Presentation] 脊髄の排便中枢を介するグレリンの結直腸運動亢進作用 (2007)
  • Author(s): 志水泰武, S Anthony, 椎名貴彦, 武脇義, Furness JB.
  • Organizer: 第49回日本平滑筋学会
  • Place of Presentation: 奈良
  • Year and Date: 2007-07-05
• [Book] Chapter 2: The enteric nervous system and its extrinsic connections. (2008)
  • Author(s): Furness, J. B., Nguyen, T. V., Nurgali, K., Shimizu, Y.
  • Total Pages: 15-39
  • Publisher: Lippincott