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2008 Fiscal Year Final Research Report

Development of the prominent labeling reagent for LC/MS/MS and its application to the analysis of the biomolecules

Research Project

  • PDF
Project/Area Number 19590036
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionThe University of Tokyo

Principal Investigator

SANTA Tomofumi  The University of Tokyo, 大学院・薬学系研究科, 准教授 (30187306)

Project Period (FY) 2007 – 2008
Keywords分析化学
Research Abstract

近年、高速液体クロマトグラフィー(LC)とタンデム型質量分析器(MS/MS)を組み合わせたLC/MS/MS法が広く用いられている。本研究では、LC/MS/MS法における検出感度および選択性の向上を目的とした標識試薬を合成した。また、合成した標識試薬を用いて疾患のマーカーとなる生体分子の高感度な分析法を開発した。本研究で開発した分析法は、新生児のマススクリーニング法として海外で用いられている。

  • Research Products

    (13 results)

All 2008 2007

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (7 results)

  • [Journal Article] Synthesis of 4-[2-(N, N-dimethylamino) ethylamino sulfonyl]-7-N-methylhydrazino-2, 1, 3-ben zoxadiazole (DAABD-MHz) as a derivatization reagent for aldehydes in liquid chromatography/electrospray ionization-tandem mass spectrometry2008

    • Author(s)
      Santa T, Al-Dirbashi OY, Ichibangase T, Rashed MS, Fukushima T, Imai K.
    • Journal Title

      Biomed. Chromatogr. 22

      Pages: 115-118

    • Peer Reviewed
  • [Journal Article] Al-Makhadab M Rapid UPLC-MS/MS method for routine analysis of plasma pristanic, phytanic and very-long chain fatty acid markers of peroxisomal disorders2008

    • Author(s)
      Al-Dirbashi OY, Santa T, Rashed MS, Al-Hassnan Z, Shimozawa N, Chedrawi AK, Jacob M
    • Journal Title

      J. Lipid Res. 49

      Pages: 1855-1862

    • Peer Reviewed
  • [Journal Article] Rcent progress in the development of derivatization reagents having a benzofurazan structure2008

    • Author(s)
      Santa T, Fukushima T, Ichibangase T, Imai K.
    • Journal Title

      Biomed. Chromatogr. 22

      Pages: 343-353

    • Peer Reviewed
  • [Journal Article] Synthesis of benzofurazan derivatization reagents for carboxylic acids in liquid chromatography/ electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS)2007

    • Author(s)
      Santa T, Al-Dirbashi OY, Ichibangase T, Fukushima T, Rashed MS, Funatsu T, Imai K.
    • Journal Title

      Biomed. Chromatogr. 21

      Pages: 1207-1213

    • Peer Reviewed
  • [Journal Article] Derivatization reagents in liquid chromatography/electrospray ionization tandem mass spectrometry for biomedical analysis2007

    • Author(s)
      Santa T, Al-Dirbashi OY, Fukushima T.
    • Journal Title

      Drug Disc. Ther. 1

      Pages: 108-118

    • Peer Reviewed
  • [Journal Article] Analysis of organic acid markers relevant to inherited metabolic diseases by ultra-performance liquid chromatography/tandem mass spectrometry as benzofurazan derivatives2007

    • Author(s)
      Al-Dirbashi OY, Santa T, Al-Qahtani K, Al-Amoudi M, Rashed MS.
    • Journal Title

      Rapid Commun. Mass Spectrom. 21

      Pages: 1984-1990

    • Peer Reviewed
  • [Presentation] Development of benzofurazan derivatization reagents for LC-MS/MS and application to the analysis of bio-markers of peroxisomal disorders2008

    • Author(s)
      Al-Dirbashi OY, Santa T, Shimozawa N, Rashed MS.
    • Organizer
      Japan-China Joint Medicine Workshop on Drug Discoveries and Therapeutics
    • Place of Presentation
      Tokyo
    • Year and Date
      20080929-20081001
  • [Presentation] Synthesis of benzofurazan derivatization reagent and analysis of fatty acids by LC/FL and LC/MS2008

    • Author(s)
      Santa T, Osama Y Al-Dirbashi.
    • Organizer
      ISLS 2008 (XIII International Synposium on Luminescence Spectrometry)
    • Place of Presentation
      Bologna
    • Year and Date
      20080907-20080911
  • [Presentation] ベンゾフラザン骨格を有するLC/MS/MS用標識試薬の開発と疾患マーカーの高感度分析2008

    • Author(s)
      三田智文, Osama Y Al-Dirbashi
    • Organizer
      第15回クロマトグラフィーシンポジウム
    • Place of Presentation
      静岡
    • Year and Date
      20080529-20080531
  • [Presentation] ベンゾフラザン骨格を有するLC/MS/MS用標識試薬の開発と応用2008

    • Author(s)
      三田智文, Osama Y Al-Dirbashi
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Year and Date
      20080328-20080330
  • [Presentation] Novel diagnostic methods for glutaric acidemia type 12007

    • Author(s)
      Al-Dirbashi OY, Rashed MS, Santa T, Al-Hassnan Z
    • Organizer
      SSIEM2007 (Society for the Study of Inborn Errors of Metabolism)
    • Place of Presentation
      Hamburg
    • Year and Date
      20070904-20070907
  • [Presentation] Development of benzofurazan labeling reagents for LC/MS/MS and their application to biomarker analysis.2007

    • Author(s)
      Santa T, Al-Dirbashi OY, Rashed MS
    • Organizer
      6^<th> AFMC International Medicinal Chemistry Symposium
    • Place of Presentation
      Istanbul
    • Year and Date
      20070708-20070711
  • [Presentation] Development of benzofurazan labeling reagents for LC/MS/MS and their application to biomarker analysis2007

    • Author(s)
      Santa T, Al-Dirbashi OY, Rashed MS
    • Organizer
      China-Japan Joint Medicine Workshop on Drug Discoveries and Therapeutics
    • Place of Presentation
      Jinan
    • Year and Date
      20070526-20070530

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Published: 2011-06-18   Modified: 2016-04-21  

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