2009 Fiscal Year Final Research Report
Characteristics and regulatory mechanisms of H^+ pump currents through the plasma membrane vacuolar H^+-ATPases in osteoclasts
| Project/Area Number |
19590213
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Osaka City University |
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Principal Investigator |
SAKAI Hiromu Osaka City University, 大学院・医学研究科, 研究員 (90382192)
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| Co-Investigator(Kenkyū-buntansha) |
KUNO Miyuki 大阪市立大学, 大学院・医学研究科, 准教授 (00145773)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 生理学 / 細胞・組織 / シグナル伝達 / プロトンポンプ / 破骨細胞 / 骨リモデリング |
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| Research Abstract |
Osteoclasts resolve bone tissues by secreting acids and proteolytic enzymes and thus play significant roles in bone remodeling. We succeeded in a real-time measurements of proton currents through the plasma membrane vacuolar H^+-ATPase (V-ATPase) using the whole-cell clamp technique. The V-ATPase current was increased by intracellular acidification and depolarization, and inhibited rapidly by exposure to high concentrations of extracellular Ca^<2+>, a negative feedback signal for osteoclast functions. The underlying mechanisms of the Ca^<2+>-sensing responses were investigated.
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[Journal Article] Temperature dependence of proton permeation through a voltage-gated proton channel.2009
Author(s)
Kuno, M., Ando, Morihata, H., Sakai, H., Mori, H., Sawada, M., Oiki, S
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Journal Title
J. Gen. Physiol 134(3)
Pages: 191-205
Peer Reviewed
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[Journal Article] Early and late activation of the voltage-gated proton channel during lactic acidosis through pH-dependent and-independent mechanisms. Pflugers Arhciv-Eur.2008
Author(s)
Morihata, H. Kawawaki, J., Okina, M., Sakai, H., Notomi, T., Sawada, M., Kuno, M
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Journal Title
J. Physiol 455(5)
Pages: 829-838
Peer Reviewed
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[Presentation] Temperature dependence of proton permeation through a volage-gated proton channel in microglia.2010
Author(s)
Kuno, M., Ando, H., Morihata, H., Sakai, H., Mori, H., Oiki, S
Organizer
54th Annual Meeting
Place of Presentation
San Francisco, CA
Year and Date
20100220-20100224
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[Presentation] Temperature-dependence of proton permeation through a voltage-gated proton channel in microglia.2009
Author(s)
Kuno, M., Ando, H., Morihata, H., Sakai, H., Mori, H., Shimizu, H., Iwamoto, I., Oiki, S
Organizer
36th International Congress of Physiological Sciences
Place of Presentation
Kyoto
Year and Date
20090727-20090801
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[Presentation] Constitutive and Ca2+-stijulated turnover of the plasma membrane vacuolar H+-ATPase (V-ATPase) in murine osteoclasts.2009
Author(s)
Sakai, H., Notomi, T., Moriura, Y., Kawawaki, J., Kuno, M
Organizer
53th Annual Meeting
Place of Presentation
Boston, Massatsusetts
Year and Date
20090228-20090304
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[Presentation] Early and Late Activation of the Voltage-Gated Proton Channel during Lactic Acidosis through pH-Dependent and -Independent Mechanisms.2008
Author(s)
Morihata, H., Kawawaki, J., Okina, M., Sakai, H., Notomi, T., Kuno, M
Organizer
52th Annual Meeting
Place of Presentation
Long Beach, California
Year and Date
20080202-20080206
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