Application of RNA interference for therapeutic approach to refractory respiratory diseases

2009 Fiscal Year Final Research Report

• Project/Area Number: 19590896
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Hiroshima University
• Principal Investigator: HATTORI Noboru Hiroshima University, 大学院・医歯薬学総合研究科, 准教授 (00283169)
• Co-Investigator(Renkei-kenkyūsha): YOKOYAMA Akihito 高知大学, 医学部, 教授 (30191513) ; ISHIKAWA Nobuhisa 広島大学, 大学院・医歯薬学総合研究科, 助教 (90423368)
• Project Period (FY): 2007 – 2009
• Keywords: RNA干渉 / 肺線維症 / 肺癌 / 気管支喘息 / GFP

Research Abstract

In the present study, we tried to determine whether intratracheal administration of siRNA against green fluorescence protein (GFP) could inhibit gene expression of GFP in the lungs of GFP-transgenic mouse or in the lung tumor which was generated by the intrathoracic inoculation of Lewis lung adenocarcinoma (LLC) cells over-expressing GFP (GFP-LLC). To detect fluorescence from GFP in the lung, Biozero fluorescence microscope (Keyence, Osaka, Japan) was used. The strong fluorescence signals were observed both in the lung of GFP transgenic mice and in the lung tumor consisting of GFP-LLC cells. However, intratracheal administration of siRNA against GFP did not inhibit the degree of florescence signal. The amount of siRNA used in the study might be insufficient.

Research Products

• [Journal Article] Perceived stress, severity of asthma, and quality of life in young adults with asthma. (2009)
  • Author(s): K imura T, Yokoyama A, Kohno N, Nakamura H, Eboshida A
  • Journal Title: Allergol Int 58 Pages: 71-79
  • Peer Reviewed
• [Journal Article] IL-12p40 is essential for the down-regulation of airway hyperresponsiveness in a mouse model of bronchial asthma with prolonged antigen exposure. (2009)
  • Author(s): O nari Y, Yokoyama A, Haruta Y, Nakashima T, Iwamoto H, Hattori N, Kohno N
  • Journal Title: Clin Exp Allergy 39 Pages: 290-298
  • Peer Reviewed
• [Journal Article] Airflow limitation in smokers is associated with subclinical atherosclerosis (2009)
  • Author(s): I wamoto H, Yokoyama A, Kitahara Y, Ishikawa N, Haruta Y, Yamane K, Hattori N, Hara H, Kohno N
  • Journal Title: Am J Respir Crit Care Med 179 Pages: 35-40
  • Peer Reviewed
• [Journal Article] Suplatast tosilate prevents bleomycin-induced pulmonary fibrosis in mice. (2009)
  • Author(s): F uronaka M, Hattori N, Tanimoto T, Senoo T, Ishikawa N, Fujitaka K, Haruta Y, Yokoyama A, Kohno N
  • Journal Title: J Pharmacol Exp Ther 328 Pages: 55-61
  • Peer Reviewed
• [Journal Article] Suppressor of cytokine signaling 1 inhibits pulmonary inflammation and fibrosis (2008)
  • Author(s): N akashima T, Yokoyama A, Onari Y, Shoda H, Haruta Y, Hattori N, Naka T, Kohno N
  • Journal Title: J Allergy Clin Immunol 121 Pages: 1269-1276
  • Peer Reviewed
• [Journal Article] Anti-inflammatory effects and clinical efficacy of theophylline and tulobuterol in mild-to-moderate chronic obstructive pulmonary disease. (2008)
  • Author(s): K anehara M, Yokoyama A, Tomoda Y, Shiota N, Iwamoto H, Ishikawa N, Taooka Y, Haruta Y, Hattori N, Kohno N
  • Journal Title: Pulm Pharmacol Ther 21 Pages: 874
  • Peer Reviewed
• [Journal Article] Usefulness of monitoring the circulating Krebs von den Lungen-6 levels to predict the clinical outcome of patients with advanced nonsmall cell lung cancer treated with epidermal growth factor receptor tyrosine kinase inhibitors. (2008)
  • Author(s): Ishikawa N, Hattori N, Yokoyama A, Tanaka S, Nishino R, Yoshioka K, Ohshimo S, Fujitaka K, Ohnishi H, Hamada H, Arihiro K, Kohno N.
  • Journal Title: Int J Cancer 122 Pages: 2612-2620
  • Peer Reviewed
• [Journal Article] Circulating KL-6/MUC1 as an independent predictor for disseminated intravascular coagulation in acute respiratory distress syndrome. (2008)
  • Author(s): N akashima T, Yokoyama A, Ohnishi H, Hamada H, Ishikawa N, Haruta Y, Hattori N, Tanigawa K, Kohno N
  • Journal Title: J Intern Med 263 Pages: 432-439
  • Peer Reviewed
• [Journal Article] Cancer-testis antigen lymphocyte antigen 6 complex locus K is a serologic biomarker and a therapeutic target for lung and esophageal carcinomas. (2007)
  • Author(s): Ishikawa N, Takano A, Yasui W, Inai K, Nishimura H, Ito H, Miyagi Y, Nakayama H, Fujita M, Hosokawa M, Tsuchiya E, Kohno N, Nakamura Y, Daigo Y.
  • Journal Title: Cancer Res 1567 Pages: 11601-11611
  • Peer Reviewed
• [Journal Article] Activation of KIF4A as a prognostic biomarker and therapeutic target for lung cancer. (2007)
  • Author(s): T aniwaki M, Takano A, Ishikawa N, Yasui W, Inai K, Nishimura H, Tsuchiya E, Kohno N, Nakamura Y, Daigo Y
  • Journal Title: Clin Cancer Res 13 Pages: 6624-6631
  • Peer Reviewed
• [Journal Article] Differential expression of the glycosylated forms of MUC1 during lung development. (2007)
  • Author(s): S akurai J, Hattori N, Nakajima M, Moriya T, Suzuki T, Yokoyama A, Kohno N
  • Journal Title: Eur J Histochem 51 Pages: 95-102
  • Peer Reviewed
• [Journal Article] Adenoviral transduction of MRP-1/CD9 and KAI1/CD82 inhibits lymph node metastasis in orthotopic lung cancer model. (2007)
  • Author(s): T akeda T, Hattori N, Tokuhara T, Nishimura Y, Yokoyama M, Miyake M
  • Journal Title: Cancer Res 67 Pages: 1744-1749
  • Peer Reviewed