2009 Fiscal Year Final Research Report
Development of Imaging technique for Alzheimer's disease
Project/Area Number |
19689002
|
Research Category |
Grant-in-Aid for Young Scientists (A)
|
Allocation Type | Single-year Grants |
Research Field |
Physical pharmacy
|
Research Institution | Kyushu University |
Principal Investigator |
YAMADA Kenichi Kyushu University, 大学院・薬学研究院, 准教授 (60346806)
|
Project Period (FY) |
2007 – 2009
|
Keywords | アミロイド / フリーラジカル / 磁気共鳴 / ニトロキシルラジカル |
Research Abstract |
Alzheimer's disease is most common neurodegenerative disorder, which is characterized by progressive memory deficit, cognitive impairment and personal changes. The main pathogenesis is thought extracellular deposits of a short peptide referred to as amyloid β (Aβ), which is major component of the plaque. However, the molecular mechanism for Aβ aggregation process is not well defined. Herein, the aim of this study was to develop new Aβ binding nitroxide to detect fibrils and understand an aggregation process. Aβ binding nitroxide was designed from 6-methoxybenzothiazoleaniline. Fluorescence and ESR spectroscopic analysis demonstrated that the nitroxide banded to Aβ fibrils. Further, we succeeded in detecting of fibrillization process by analyzing the change of ESR spectrum. Another advantage of Aβ binding nitroxide was an antioxidant effect. These findings indicated that new nitroxide can detect Aβ fibrils and evaluate an aggregation process with ESR spectroscopy.
|
Research Products
(16 results)
-
[Journal Article] Effective 2, 6-substitution of piperidine nitroxyl radical by carbonyl compound.2010
Author(s)
Sakai, K., Yamada, K., Yamasaki, T., Kinoshita, Y., Mito, F., Utsumi, H.
-
Journal Title
Tetrahedron 66
Pages: 2311-2315
Peer Reviewed
-
[Journal Article] Dynamic nuclear polarization studies of redox-sensitive nitroxyl spin probes in liposomal solution.2010
Author(s)
Benial, AM., Utsumi, H., Ichikawa, K., Murugesan, R., Yamada, K., Kinoshita, Y., Naganuma, T., Kato, M.
-
Journal Title
J. Magn. Reson. 204(1)
Pages: 131-8
Peer Reviewed
-
-
[Journal Article] Noninvasive assessment of the brain redox status after transient middle cerebral artery occlusion using Overhauser-enhanced magnetic resonance imaging.2009
Author(s)
amato, M., Shiba, T., Yamada, K,, Watanabe, T., Utsumi, H
-
Journal Title
J. Cereb. Blood Flow Metab. 29(10)
Pages: 1655-1664
Peer Reviewed
-
[Journal Article] Development of novel nitroxyl radicals for controlling reactivity with ascorbic acid.2009
Author(s)
Kinoshita, Y., Yamada, K., Yamasaki, T., Sadasue, H., Sakai, K., Utsumi, H.
-
Journal Title
Free Radic. Res. 43
Pages: 565-571
Peer Reviewed
-
-
[Journal Article] Synthesis of nitroxyl radicals for Overhauser-enhanced magnetic resonance imaging.2008
Author(s)
Yamada, K., Kinoshita, Y., Yamasaki, T., Sadasue, H., Mito, F., Nagai, M., Matsumoto, S., Aso, M., Suemune, H., Sakai, K., Utsumi, H
-
Journal Title
Arch. Pharm. 341
Pages: 548-553
Peer Reviewed
-
[Journal Article] Formation of TEMPOL-hydroxylamine during reaction between TEMPOL and hydroxyl radical: HPLC/ECD study.2008
Author(s)
Kudo, W., Yamato, M., Yamada, K., Kinoshita, Y., Shiba, T., Watanabe, T., Utsumi, H.
-
Journal Title
Free Radic. Res. 42
Pages: 505-512
Peer Reviewed
-
[Journal Article] Analysis of nitroxyl spin probes in mouse brain by X-band ESR with microdialysis technique2008
Author(s)
Shiba, T., Yamato, M., Kudou, W., Ichikawa, K., Yamada, K., Watanabe, T., Utsumi, H.
-
Journal Title
J. Pharm. Sci. 97
Pages: 4101-4107
Peer Reviewed
-
-
[Presentation] Stability Evaluation for 2, 6-substituted TEMPO Derivatives in the Reduction Process.2008
Author(s)
Kinoshita, Y., Yamada, K., Sadasue, H., Yamasaki, T., Sakai, K., Utsumi, H.
Organizer
SPIN 2008: 5th International Conference on Nitroxide Radicals.
Place of Presentation
Ancona, Italy
Year and Date
20080907-20080911
-
-
-
-
-