2009 Fiscal Year Final Research Report
Development of growth inhibition technology of toxic-producing blue-green algae
| Project/Area Number |
19760370
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
HIDAKA Taira Kyoto University, 大学院・工学研究科, 助教 (30346093)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 水質汚濁・土壌汚染防止・浄化 / モデル化 / 有害化学物質 |
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| Research Abstract |
Applicability of real-time PCR method was evaluated by cultivation experiments of Microcystis. After logarithmic growth phase, quantification results by both mcy A gene and 16S rRNA gene were similar to cell numbers measured by microscopic analysis. Increase in cell number and mcy A gene corresponded with increase in microcystin production. Simultaneous increase in mcy A gene and microcystin was observed in a field survey. These results proved that real-time PCR quantification can be applied to microcystin-producing blue-green algae, and it is promising to develop monitoring and growth inhibition technologies of toxic-producing blue-green algae.
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