2009 Fiscal Year Final Research Report
Analysis of molecular mechanisms for germ cell formation in an ascidian, Ciona intestinalis
| Project/Area Number |
19770203
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KURABAYASHI Maki (白江 麻貴) The Institute of Physical and Chemical Research, 生殖系列研究チーム, 研究員 (60391942)
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| Project Period (FY) |
2007 – 2009
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| Keywords | カタユウレイボヤ / 生殖細胞 / 生殖質 / Vasa / Tudor / TDRD7 / 転写抑制 / Groucho |
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| Research Abstract |
In this study, I found that the protein products of two postplasmic/PEM RNA genes Ci-PEM (Ci-pem-1) and Ci-TDRD7 (Ci-pem-11) have crucial roles in germline formation of Ciona intestinalis respectively. The protein product of Ci-PEM was distributed in the nucleus in the germline blastomeres as well as the postplasm in the cleavage stage and required for the repression of somatic gene expression in the germline. Ci-TDRD7 protein formed a complex with CiVH, a homolog of the conserved germ cell marker Vasa, in the germline cells, and it was involved in the formation of the peri-nuclear CiVH granules in the primordial germ cells (PGCs) after gastrulation.
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