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2008 Fiscal Year Final Research Report

Ubiquitin-dependent regulation of DNA replication arrest

Research Project

  • PDF
Project/Area Number 19810007
Research Category

Grant-in-Aid for Young Scientists (Start-up)

Allocation TypeSingle-year Grants
Research Field Risk sciences of radiation/Chemicals
Research InstitutionKyoto University

Principal Investigator

MOTEGI Akira  Kyoto University, 大学院・医学研究科, 助教 (80452332)

Project Period (FY) 2007 – 2008
Keywords組換え修復 / 複製後修復 / ユビキチン化
Research Abstract

本研究では、ヒトユビキチンリガーゼHLTFが、出芽酵母Rad5の機能的ホモログであり、PCNAのポリユビキチン化を介して、複製ポリメラーゼの停止解除を促進することを明らかにした(PNAS 2008論文発表)。また、 DNA二本鎖切断の修復の際にUBC13と共役して働くユビキチンリガーゼの一つRNF8の遺伝子破壊DT40株を作製し、その表現型解析を行った。これにより、RNF8が相同組み替え修復を促進することを明らかにした(Keystone Symposia 2009学会発表)。

  • Research Products

    (15 results)

All 2009 2008 2007

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (9 results)

  • [Journal Article] 抗がん剤で考えるDNA組み換えの制御機構2009

    • Author(s)
      茂木章, 武田俊一
    • Journal Title

      タンパク質・核酸・酵素別冊 54(4)

      Pages: 450-458

  • [Journal Article] Roles of proteasome in DNA repair : impacts of proteasome inhibiotors on cancer therapy2008

    • Author(s)
      Akira Motegi, Yasuhiro Murakawa, and Shunichi Takeda
    • Journal Title

      Cancer lett (in press)

    • Peer Reviewed
  • [Journal Article] Lysine 63-linked polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks2008

    • Author(s)
      Akira Motegi, Hung-jiun Liaw, Kyoo-Young Lee, Henk P. Roest, Alex Maas, Xiaoli Wu, Helen Moinova, Sanford D. Markowitz, Hao Ding, Jan H. J. Hoeijmakers, and Kyungjae Myung
    • Journal Title

      Proc. Natl. Acad. Sci. USA 105

      Pages: 12411-12416

    • Peer Reviewed
  • [Journal Article] Mph1 promotes gross chromosomal rearrangement through partial inhibition of homologous recombination J2008

    • Author(s)
      Soma Banerjee, Stephanie Smith, Ji-Hyun Oum, Hung-Jiun Liaw, Ji-Young Hwang, Nilabja Sikdar, Akira Motegi, Sang Eun Lee, and Kyungjae Myung
    • Journal Title

      Cell Biol 181

      Pages: 1083-1093

    • Peer Reviewed
  • [Journal Article] ユビキチン化によるDNA修復の制御機構2008

    • Author(s)
      茂木章, 村川泰裕, 武田俊一
    • Journal Title

      実験医学別冊 26(2)

      Pages: 62-70

  • [Journal Article] ユビキチン化によるDNA修復の制御機構2007

    • Author(s)
      茂木章, 村川泰裕, 武田俊一
    • Journal Title

      細胞工学 26(11)

      Pages: 1290-1295

  • [Presentation] Functional analysis of the ubiquitin ligase RNF8 in DNA repair Keystone Symposia2009

    • Author(s)
      Saho Era, Yohei Kasaishi, Shunichi Takeda, and Akira Motegi
    • Organizer
      Genome Instability and Repair
    • Place of Presentation
      Taos, NM, USA
    • Year and Date
      20090301-06
  • [Presentation] Lysine 63-linked polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks, Gordon Research Conference2009

    • Author(s)
      Akira Motegi, Hung-jiun Liaw, Kyoo-Young Lee, Henk P. Roest, Alex Maas, Xiaoli Wu, Helen Moinova, Sanford D. Markowitz, Hao Ding, Jan H. J. Hoeijmakers, and Kyungjae Myung
    • Organizer
      Mammalian DNA Repair
    • Place of Presentation
      Ventura, CA, USA
    • Year and Date
      20090208-13
  • [Presentation] Human ubiquitin ligases HLTF and SHPRH prevents genomic instability from stalled replication forks, Gordon Research Conference2009

    • Author(s)
      Hung-jiun Liaw, Akira Motegi, Kyoo-Young Lee, Henk P. Roest, Alex Maas, Xiaoli Wu, Helen Moinova, Sanford D. Markowitz, Hao Ding, Jan H. J. Hoeijmakers, and Kyungjae Myung
    • Organizer
      Mammalian DNA Repair
    • Place of Presentation
      Ventura, CA, USA
    • Year and Date
      20090208-13
  • [Presentation] Chicken Rap80 promotes repair of toposiomerase II poison-induced DNA damage, Gordon Research Conference2009

    • Author(s)
      Junko Iijima, Akira Motegi, Toru Fukushima, Yoshihito Taniguchi, and Shunichi Takeda
    • Organizer
      Mammalian DNA Repair
    • Place of Presentation
      Ventura, CA, USA
    • Year and Date
      20090208-13
  • [Presentation] Lysine 63-linked polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks2008

    • Author(s)
      Akira Motegi, Hung-jiun Liaw, Kyoo-Young Lee, Henk P. Roest, Alex Maas, Xiaoli Wu, Helen Moinova, Sanford D. Markowitz, Hao Ding, Jan H. J. Hoeijmakers, Shunichi Takeda, and Kyungjae Myung
    • Organizer
      The 6th International 3R Symposium 2008
    • Place of Presentation
      Tsumagoi, Japan
    • Year and Date
      20081027-30
  • [Presentation] Human SHPRH and HLTF suppress genomic instability through the lysine-63-linked polyubiquitination of proliferating cell nuclear antigen(PCNA)2008

    • Author(s)
      Akira Motegi, Hung-jiun Liaw, Raman Sood, Helen Moinova, Sanford D. Markowitz, Pu Paul Liu, Kyungjae Myung, and Shunichi Takeda
    • Organizer
      The International Ataxia-Telangiectasia Workshop2008
    • Place of Presentation
      Otsu, Japan
    • Year and Date
      20080422-26
  • [Presentation] Human PTIP Is Implicated in a Late Step of Homologous Recombination-mediated Double Strand Breaks(DSBs)Repair2008

    • Author(s)
      Xin Wang, Akira Motegi, and Shunichi Takeda
    • Organizer
      The International Ataxia-Telangiectasia Workshop 2008
    • Place of Presentation
      Otsu, Japan
    • Year and Date
      20080422-26
  • [Presentation] Human post-replication repair pathway by the Rad5 homolog SHPRH in the suppression of genomic instability2008

    • Author(s)
      Hungjiun Liaw, Akira Motegi, Kyoo-Young Lee, and Kyungjae Myung
    • Organizer
      Keystone Symposia (Genome Instability and Repair)
    • Place of Presentation
      Santa Fe, NM, USA
    • Year and Date
      20080210-15
  • [Presentation] Human SHPRH and HLTF suppress genome instability through PCNA polyubiquitination2007

    • Author(s)
      Akira Motegi, Hungjiun Liaw, Kyoo-Young Lee, Shunichi Takeda, and Kyungjae Myung
    • Organizer
      2007 Annual Meeting of Molecular Biology Society of Japan(Symposium)
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      20071211-15

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Published: 2010-06-10   Modified: 2016-04-21  

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