2021 Fiscal Year Final Research Report
Pregnancy-specific responses in bovine extra uterine organs
| Project/Area Number |
19H03099
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 42010:Animal production science-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
窪 友瑛 地方独立行政法人北海道立総合研究機構, 農業研究本部 酪農試験場, 研究職員 (50825338)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | ウシ / 妊娠認識 / 子宮頚管 / インターフェロンタウ / インターフェロン誘導遺伝子 |
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| Outline of Final Research Achievements |
The purpose of this study was to clarify the bovine pregnancy response in extrauterine tissues temporally and spatially and to elucidate the kinetics of the involvement of interferon (IFN)τ produced by the embryo. We first detected IFNτ in the cervical mucus as well as the pregnancy-dependent increase in IFN-induced gene expression in the cervix around 18 days of pregnancy, when preimplantation bovine embryos are at their peak production of IFNτ in the uterus. In addition, RNA sequence analysis of cervical tissue has revealed a marked increase in IGFBP3 expression just before ovulation. These results suggest the possibility of detecting pregnancy- and estrus-related factors in cervical tissues that can be collected less-invasively, and the development of early pregnancy and estrus detection technology is expected.
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| Free Research Field |
家畜繁殖学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究より、着床前に子宮内で成長しながら妊娠認識物質であるIFNτの産生が最大になる18日前後の早期妊娠時で低侵襲かつ簡易迅速に採取可能な頸管組織における妊娠特異的な遺伝子発現および子宮内のIFNτの直接検出を初めて明らかにした。加えて、頸管における発情特異的な発現を示すIGFBP3の遺伝子、蛋白の検出の可能性も提起することができた。本成果は、子宮組織や胚に影響を及ぼすことなく子宮内の胚の直接存在が検出可能であることを国内外での初知見である。今後は、妊娠特異的な発現遺伝子の検出と低侵襲的な早期妊娠検出技術開発への指標として期待されるとともに、発情検出に係る指標の検出と有用性も期待される。
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