Genetic analysis of the pollen-stigma incompatibility in Brassica rapa.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K05963
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: Tohoku University
• Principal Investigator: Takada Yoshinobu 東北大学, 生命科学研究科, 技術専門職員 (30451610)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: アブラナ科植物 / 自家不和合性 / 一側性不和合性 / 柱頭・花粉情報伝達 / 有性生殖 / F1採種

Outline of Final Research Achievements

This study aimed to elucidate the incompatibility mechanism during pollination in Brassica rapa by explaining the causal genes from self-compatible mutants and genetically characterizing a novel unilateral incompatibility (UI) phenomenon. Mapping of two different self-compatible mutant lines revealed the presence of causal factors on chromosomes 7 and 10 of Brassica rapa, respectively. Genetic analysis of male and female parents showing a novel UI revealed that the regulating genes locate at the previously reported unilaterally incompatibility locus. This result suggests complex incompatibility recognition may be established by sequence polymorphisms of the UI-regulating genes.

Free Research Field

植物育種学

Academic Significance and Societal Importance of the Research Achievements

自家和合性変異体の解析より明らかになった遺伝子座はいずれも新規因子と考えられた。また、一側性不和合性制御遺伝子の多型により新規な組み合わせでの不和合性現象が生じることが予測され、今後特定の組合せで受粉時不和合性を生み出すことができるようになるかもしれない。これら技術は、ハクサイ・カブ等アブラナ科野菜のF1採種効率を上げることに貢献すると考えられる。以上より、今後のアブラナ科植物受粉時不和合性研究において極めて重要な進展を遂げることができた。