2020 Fiscal Year Research-status Report
How do proteoglycans mediate auxin gradient sensing in planar cell polarity?
| Project/Area Number |
19K06701
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| Research Institution | Hokkaido University |
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Principal Investigator |
Teh Ooikock 北海道大学, 高等教育推進機構, 助教 (80791277)
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| Co-Investigator(Kenkyū-buntansha) |
藤田 知道 北海道大学, 理学研究院, 教授 (50322631)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | cell wall / Physcomitrium patens / auxin response factors / proteoglycan |
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| Outline of Annual Research Achievements |
In the past one year, we have made interesting progress regarding ARFC, a putative downstream effector for SB. As a transcription factor, we suspect that ARFC may regulate a range of transcriptional targets to mediate cell wall remodelling. To identify these targets, we performed RNA sequencing using an ARFC inducible line and pinpoint a range of cell wall related genes such as pectin methylesterases and expansins. We found that these genes are repressed by ARFC through Gene Ontology analyses. At the same time, through collaboration with Prof. Kotake (Saitama University) we have also confirmed that the cell wall compositions of ARFC knock-out mutants and ARFC overexpressor have changed, establish a link between ARFC and cell wall remodelling. In order to understand the expression pattern of ARFC, we have generated a promoter fusion reporter for ARFC. Using confocal imaging, we determined that ARFC expression is excluded from the stem cell. In our effort to prepare the sequencing population for our repressor mutants, Prince Charming (PC), we have established the somatic hybridization protocol in the lab. Results from my work has been presented at the Annual meeting of the Japanese Society of Plant Physiologists.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The progress is so far in line with the plans set out in this proposal. Unexpected finding on the function of ARFC has created new avenues to design experiments that are more relevant. We are now focusing on understanding the connection between SB, ARFC and cell wall remodeling.
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| Strategy for Future Research Activity |
IN the next one year, we will continue to investigate how SB as a proteoglycan can affect cell wall remodeling through the ARFC effector. We will be analysing the cell wall components by HPLC in SB and ARFC knock-out mutants.
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| Causes of Carryover |
The spending efficiency for this fiscal year is in line with the original plan. Incurring amount for next fiscal year is due to the travel restriction caused by pandemic.
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Research Products
(3 results)
