Development of a separation-field library for two-dimensional LC to drive dramatic progress of chiral metabolomics

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K07027
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
• Research Institution: Kyushu University
• Principal Investigator: Akita Takeyuki 九州大学, 薬学研究院, 講師 (50294963)
• Co-Investigator(Kenkyū-buntansha): 濱瀬 健司 九州大学, 薬学研究院, 教授 (10284522)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 分析化学 / メタボロミクス / 光学分割 / アミノ酸 / 固定相 / キラルセレクター / HPLC

Outline of Final Research Achievements

In order to promote the elucidation of the existence, distribution, and function of trace amount of enantiomers in the living body, which has been difficult until now, and to accelerate the search for seeds in medicine, we synthesized chiral stationary phases with various chiral recognition sites and developed a two-dimensional LC separation-field library composed of chiral-separation columns with various separation characteristics. By constructing a two-dimensional HPLC system that utilizes the separation characteristics of each column in the library, we performed highly sensitive and highly selective analyses of chiral amino acids, etc. in various real samples. Furthermore, for the social implementation of chiral amino acid analysis, we exhibited rapid analysis by using the high-performance chiral stationary phase included in the developed library.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

本研究では多様な分離特性を持つ光学分割カラムからなる二次元LC分離場ライブラリを構築し、これを用いて、これまで困難であった生体試料中のD-アミノ酸などの微量鏡像異性体を高感度・高選択的かつ迅速に定量することが可能であることを示した。この研究成果を応用することにより、キラルメタボロミクスの飛躍的推進が可能となり、生体中における微量鏡像異性体の機能解明や、様々な疾患に対する創薬シーズや診断マーカー探索の加速が期待される。これは、新たな医薬品開発や、疾患の予防、早期発見等へとつながり、人類の健康増進に資する重要な成果である。