Study of the stem cell and inter cellular signal system underling testicular regeneration in the newt

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K07268
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 48010:Anatomy-related
• Research Institution: Hiroshima University
• Principal Investigator: Hayashi Toshinori 広島大学, 両生類研究センター, 教授 (60580925)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: イベリアトゲイモリ / 精巣 / 再生

Outline of Final Research Achievements

In this study, we published a paper that provides basic information on newt testis development and the developmental process of male germ cells, as well as the effects of day-length on testis differentiation. In addition, results from transplantation experiments showed clear evidence that newt testes are regenerated from Terminal Crest. These results are currently being prepared for submission to an international scientific journal. In addition, we have identified two steps in the formation of primordial germ cells in which the dazl gene functions. We also conducted whole genome sequencing to enhance the value of Iberian ribbed newt as a bioresource.

Free Research Field

再生生物学

Academic Significance and Societal Importance of the Research Achievements

新規のモデル動物であるイベリアトゲイモリにおいて、精巣や雄性生殖細胞の形成過程の詳細が示されたことで、イモリの生殖細胞に対する理解が進んだことに加えて、イベリアトゲイモリのモデル動物としての価値が高まった。
また、本研究から得られた知見は、マウスやゼブラフィッシュ、ツメガエルといったモデル生物と比較する基盤となるため、脊椎動物における生殖細胞分化機構の全体像を明らかにすることにも貢献する。