Mechanisms for the development of cardiac diastolic dysfunction in diabetic hearts; From the view point of Mitochondria-SR network.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K08509
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53020:Cardiology-related
• Research Institution: The University of Tokyo
• Principal Investigator: Tsushima Kensuke 東京大学, 医学部附属病院, 助教 (50436482)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 心不全 / ミトコンドリア / 筋小胞体 / 超高圧電子顕微鏡

Outline of Final Research Achievements

In this project, we tried following 2 aims.
Aim1: To develop 3D imaging of cardiac tissue using Ultra high voltage electron microscopy. : Using an ultra-high voltage electron microscope, we have established a method to reproduce the three-dimensional structure of myocardial tissue that is 10 times thicker than conventional imaging at a resolution that can withstand three-dimensional construction.
Aim2:To develop a labeling-protein using APEX2 to mark myocardial-associated membrane.: The image obtained had a high background due to the high ALP activity of myocardium. We need some improvement to decrease background.

Free Research Field

循環器病学

Academic Significance and Societal Importance of the Research Achievements

細胞を構成するオルガネラやタンパク質の3次元微細構造を理解すること、基礎研究だけでなく創薬などの臨床医学の発展に重要である。本研究で開発した手法で今後心筋の微細構造やその動態が明らかに成ることで、心不全における心筋のオルガネラや膜小胞構造の異常が明らかになることが期待される。