Effect of remote ischemic preconditioning on central nervous system regeneration

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K10298
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Osaka Dental University
• Principal Investigator: Momota Yoshihiro 大阪歯科大学, 歯学部, 教授 (60247880)
• Co-Investigator(Kenkyū-buntansha): 中込 隆之 兵庫医科大学, 医学部, 教授 (80434950)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 一過性局所脳虚血 / 神経幹細胞 / 虚血耐性 / 海馬歯状回

Outline of Final Research Achievements

We examined the effect of non-lethal remote ischemic preconditioning (RIPC) on central nervous system regeneration. CB 17 mice were divided into 3 treatment groups. The femoral artery (FA) group, the middle cerebral artery (MCA) group, and the caritid artery (CA) group had temporary occulusion and reperfusion. Following the treatment, the expression of neural stem cells was examined by immunohistochemistry. Theses findings suggest that RIPC induces endogenenous neural stem cells in the hippocampal dentate gyrus. Double staining (nestin and GFAP) further suggested the presence of astrocytes that are reactive to tissue damage. Thus, neural stem cells may be involved in regulating resistance to ischemia, and astrogria may be involved in regeneration of the nervous system.

Free Research Field

歯科麻酔学

Academic Significance and Societal Importance of the Research Achievements

不可逆的致死的脳虚血が生じる前に非致死的虚血負荷が加わることで、虚血傷害部位のみならず海馬歯状回においても神経再生能の亢進が示された。さらに、非致死的な虚血負荷が責任動脈以外の血管においても神経再生機転の亢進に関与することが明らかとなった。以上の結果から、非致死的な虚血負荷があらかじめ加わることにより致死的虚血に対し脳保護作用を獲得することが示され術後のリハビリテーションにも大きく寄与する可能性があり社会的意義は大きいものと考えられた。