2020 Fiscal Year Research-status Report
Structural-Functional Development of the Olfactory System
| Project/Area Number |
19K16261
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| Research Institution | Kyushu University |
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Principal Investigator |
LEIWE MARCUS 九州大学, 医学研究院, 助教 (80722008)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | Olfactory Bulb / Development / Pruning / Odour |
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| Outline of Annual Research Achievements |
We began by assessing the olfactory responses generated in neonatal mice. We were able to reliably record M/T cell responses from mice ranging from P2 to the adult using 2-Photon Ca2+ imaging. We assessed the difference between P2 and P6 to see if the sensitivity of glomeruli were different. P2 mice were slightly more broadly tuned than P6 mice. However, the responses were much stronger at P6 perhaps meaning that, we were able to detect more smaller events rather than actually observing a change in odour tuning.
Progress was made in being able to alter the phase code of neonatal mice. With a global KO of DA cells the consistency of the phase code was decreased. We have constructed a Cre-specific Tetanus AAV vector to see if we can reproduce these mice in healthier mice.
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| Current Status of Research Progress |
Current Status of Research Progress
4: Progress in research has been delayed.
Reason
Our 2-Photon has broken, meaning we are unable to continue with our calcium imaging experiments. However, funding seems to have been procured to purchase a new pulsing laser meaning experiments can continue soon.
Our AAV vector has been constructed and is ready for use once the laser has been repaired
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| Strategy for Future Research Activity |
1) Trial the lower concentration of odours at P2 to P6
2) Record the changes in phase coding after the injection of pAAV-Flex-TeNT-p2A-mRuby3
3) Come up with an alternative strategy if point 1) fails.
4) Try to find a way to prevent the pruning of mitral cells and see if there is an effect
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| Causes of Carryover |
Due to the pandemic and our 2-Photon failing it was impossible to do experiments for a long while. I'm only just begining to recover the time lost.
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