Three-dimensional analysis of spermatogenesis disorder

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16473
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 48010:Anatomy-related
• Research Institution: Kanazawa University
• Principal Investigator: Nakata Hiroki 金沢大学, 医学系, 講師 (80638304)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 精巣 / 精細管 / 三次元 / 三次元再構築 / 3D / 精子形成 / ブスルファン / 加齢

Outline of Final Research Achievements

We prepared mice with different degrees of spermatogenesis disorder by changing the doses of busulfan injection and different aged mice. Then, the 3D-reconstructed seminiferous tubules were analyzed to examine if the impaired or normal spermatogenesis was distributed preferentially in particular sites in seminiferous tubules or in the testis. There were 3D positions with the specific proportions common to different testes not in busulfan-induced mice but in aged mice.

Free Research Field

生殖生物学

Academic Significance and Societal Importance of the Research Achievements

薬剤または加齢という全く異なる要因において、正常または精子形成障害が起こりやすい三次元部位がそれぞれ明らかになった。本研究で得られた成果は、精子形成障害を研究する上で基礎的なデータとなり、男性の妊孕性・不妊症の研究の発展に寄与できる。