2021 Fiscal Year Final Research Report
Analysis for histological phenotype of high-risk papillary thyroid carcinoma based on molecular abnormalities
| Project/Area Number |
19K16587
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 49020:Human pathology-related
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | Papillary thyroid cancer / Ki-67 expression / TERT-promoter mutations / BRAF mutation |
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| Outline of Final Research Achievements |
The 342 surgically-resected, formalin-fixed, paraffin-embedded (FFPE) thyroid PTCs of Japanese cases were collected. All cases were evaluated for BRAF, TERT-promoter and Ki-67. Mutations in BRAF and the TERT-promoter (C228T and C250T) were analyzed with digital PCR and Ki-67 labeling index by immunohistochemistry.The clinicopathological features, including TNM stage assessed, size of tumor, and detailed histopathology features of cases were analysed.Next step is statistical analysis of prevalence and association of molecular analysis with clinicopathologic statuses.
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| Free Research Field |
人体病理学
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| Academic Significance and Societal Importance of the Research Achievements |
甲状腺癌の90％を占める乳頭癌の大部分は予後良好であり、甲状腺癌の場合、早期発見と早期治療は予後の改善に寄与しないことが明らかになっている。甲状腺癌の過剰診断や過剰治療（切除や放射性ヨード内照射療法）を抑制し、最適化するためには予後不良指標の同定が必要である。現在、Ki-67標識率やBRAF変異およびTERT promoter変異が予後を規定する有力な因子として報告されているが、一般的な病理検査室で遺伝子変異解析を日常的に行うことは困難である。本研究成果はこれらの分子異常をエヒデンスに、乳頭癌の組織像を特徴付け、日常の甲状腺がん病理診断学に汎用できる高悪性度の組織像を明らかにした。
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