2021 Fiscal Year Final Research Report
Establishment of inbred murine model of ovarian clear cell carcinoma and analysis of host immune response
| Project/Area Number |
19K18684
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56040:Obstetrics and gynecology-related
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| Research Institution | Kindai University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 卵巣癌 / 卵巣明細胞癌 / IL-6 / IL-17 / PD-L1 / マウスモデル |
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| Outline of Final Research Achievements |
In this study, the Arid1aflox/flox;(Gt)Rosa26Pik3ca*H1047R gene co-mutation was incorporated into C57BL/6 mice to establish an inbred ovarian clear cell carcinoma mouse model. We observed that administration of anti-IL-6 and anti-PD-L1 antibodies alone significantly prolonged survival in these mice, whereas combination therapy had no additive effect. In addition, mice treated with anti-IL-6 antibody showed induction of Tregs at the tumor site. Furthermore, IL-17A administration was found to activate local tumor immunity.
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| Free Research Field |
婦人科腫瘍学
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| Academic Significance and Societal Importance of the Research Achievements |
これまでの臨床試験データからは卵巣明細胞癌には免疫療法が奏功する可能性が示唆されているが、免疫応答 をin vivoで研究する動物モデルが存在しないため、その詳細については不明であった。本研究で樹立したマウスモデルは、OCCCにおける腫瘍免疫の解明に大いに役に立つと考えられる。また、抗体薬やサイトカイン投与によって得られた腫瘍免疫応答のデータは、卵巣明細胞癌に対する新たな治療法開発のベースになる。
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