Safe and efficient selection of differentiated cells by stage-specific gene expression from auto-erasable vector

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K22945
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 90:Biomedical engineering and related fields
• Research Institution: University of Tsukuba
• Principal Investigator: Nishimura Ken 筑波大学, 医学医療系, 准教授 (80500610)
• Project Period (FY): 2019-06-28 – 2021-03-31
• Keywords: 分化誘導 / 再生医療 / 創薬 / 自動除去型SeVdpベクター / miRNA

Outline of Final Research Achievements

To enhance an application of differentiated tissue to regenerative medicine and drug discovery, we need to reduce risks of tumor formation by remained undifferentiated cells and obtain highly pure differentiated cells efficiently. Adding target sequences of neural stem cell (NSC)-specific miRNA to our original auto-erasable SeVdp vector enabled us to select pure population of the induced NSCs. Moreover, we succeeded to control an expression timing of a NSC differentiation inducer from SeVdp vector by miRNA whose expression is changed at a particular period in the NSC differentiation. We plan to combine the cell selection system and the differentiation induction into single SeVdp vector to establish a system for efficient selection of highly pure differentiated cells.

Free Research Field

幹細胞工学、幹細胞生物学

Academic Significance and Societal Importance of the Research Achievements

本研究成果によって、再生医療や創薬に実用化可能な分化細胞を取得するために必要な技術の開発が進展した。この技術を完成させるためには、ベクターコピー数の微調整や、ベクター除去に用いるために最適なmiRNAの探索、試行などが必要ではあるが、そのような研究を継続して高効率高純度分化細胞選択系を確立することによって、幹細胞等から誘導して作製した分化細胞を用いた、安全な再生医療や新しい医薬の探索が可能になることが期待される。