2020 Fiscal Year Final Research Report
Elucidation of brain regions and cell types in the regulation of sleep
Project/Area Number |
19K23783
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Multi-year Fund |
Review Section |
0704:Neuroscience, brain sciences, and related fields
|
Research Institution | Asahikawa Medical College (2020) Toho University (2019) |
Principal Investigator |
Furube Eriko 旭川医科大学, 医学部, 助教 (30845566)
|
Project Period (FY) |
2019-08-30 – 2021-03-31
|
Keywords | salt induced kinase 3 / 炎症 / 睡眠 |
Outline of Final Research Achievements |
In this study, we investigated the effect of SIK3 on causing inflammatory sleep / wake abnormalities in mice by intracerebroventricular(icv) administration of LPS, which constitutes the cell wall of Gram-negative bacteria. As a result of icv administration of LPS to wild-type mice, wake time decreased, NREM delta, which is an index of sleep depth, increased, NREM sleep time increased, and REM sleep decreased significantly. On the other hand, these changes were diminished in transgenic mice with enhanced SIK3 phosphorylation capacity. Immunohistochemical examination revealed that NeuN-positive neurons expressed SIK3. In addition, as a result of icv administration of LPS , it was found that HDAC4 nuclear translocation in neurons in the lateral hypothalamic area was suppressed in wild-type mice.
|
Free Research Field |
神経解剖学
|
Academic Significance and Societal Importance of the Research Achievements |
生体が細菌やウイルスに感染すると、サイトカインの産生を促進し、発熱や食欲抑制に加えてNREM睡眠の増加・REM睡眠の減少につながる。本研究で見られたNREM睡眠の増加やREM睡眠の減少、脳波の異常も脳内でのサイトカイン濃度の変化による影響を受けたものであることが考えられる。LPS脳室内投与によるLHでのSIK3下流に存在するHDAC4の活性化の抑制は、SIK3がオレキシン神経による覚醒・睡眠制御および炎症抑制経路に関わっている可能性を示唆している。
|