2022 Fiscal Year Final Research Report
Analysis of the effect of local anesthetic on the growth of cancer cells and tumor immunity
Project/Area Number |
19K24005
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Multi-year Fund |
Review Section |
0905:Surgery of the organs maintaining homeostasis and related fields
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Research Institution | Gunma University |
Principal Investigator |
Suzuki Keiko (原口景子) 群馬大学, 医学部附属病院, 助教 (50846897)
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Project Period (FY) |
2019-08-30 – 2023-03-31
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Keywords | 局所麻酔薬 / 癌細胞株増殖抑制 / GDF-15 / TRIB3 / Ki-67 |
Outline of Final Research Achievements |
We identified that growth differentiation factor-15 (GDF-15) was commonly upregulated and Ki-67 was downregulated in lidocaine-treated cancer cell lines. In lidocaine-treated HeLa cells, increased production of GDF-15 in the endoplasmic reticulum (ER) was observed and unprocessed pro-protein form of GDF-15 was secreted extracellularly. Further, lidocaine induced apoptosis and apoptosis-inducible Tribbles homologue 3 (TRIB3) was also commonly upregulated in lidocaine-treated cancer cell lines. In addition, transcription factor C/EBP homologous protein (CHOP), which is a positive regulator of not only GDF-15 but TRIB3 was also induced by lidocaine. Lidocaine-induced growth suppression and apoptosis was suppressed by knockdown of GDF-15 or TRIB3 expression by small interference RNA (siRNA). These observations suggest that lidocaine suppresses the growth of cancer cells through increasing GDF-15 and TRIB3 expression, suggesting its potential application as cancer therapy.
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Free Research Field |
麻酔学
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Academic Significance and Societal Importance of the Research Achievements |
本研究により局所麻酔薬が抗腫瘍効果を有する可能性が示唆された。
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