2019 Fiscal Year Research-status Report
Targeting Transcription in Sarcopenia and Muscle-wasting Diseases
Project/Area Number |
19K24152
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Research Institution | Kyushu Dental College |
Principal Investigator |
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Project Period (FY) |
2019-08-30 – 2021-03-31
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Keywords | Sarcopenia / Transcription / Epigenetics / Muscle / Zfp423 |
Outline of Annual Research Achievements |
We have made significant progress in our understanding of the mechanisms by which Zfp423 regulates myoblast cell function. Using ChIP-Seq, we have constructed a detailed chromatin localization and occupancy map of Zfp423 in myoblast cells. This data has allowed us to identify several putative targets, define Zfp423 DNA-binding motifs, and candidate co-occupancy factors.
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Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Currently, we are in the process of evaluating the effects of Zfp423 binding at putative targets in muscle cells. In addition, generation and characterization of Zfp423 conditional knockout mice is ongoing.
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Strategy for Future Research Activity |
We will conditionally delete Zfp423 in in satellite cells using Pax7-Cre and perform a complete analysis of muscle and satellite cell functions in vivo and ex vivo at 3 weeks, 7 months and 18 months of age. Muscle mass, size and morphology will be analysed by histomorphometry and DEXA densitometry. Immunohistological staining will be used to quantify SC pool size and proliferative capacity. Ex vivo assessment of primary SCs and myofiber culture assays will be used to identify cell-autonomous changes in candidate signaling pathways and target gene expression.
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Causes of Carryover |
We were able to reduce expenditure and costs of consumable materials by advancing our experiments in a more efficient manner.
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Research Products
(1 results)