2023 Fiscal Year Final Research Report
Novel RNA-seq analysis reveals RNA regulatory networks in bacteria
| Project/Area Number |
19KK0406
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| Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research (A))
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2020 – 2023
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| Keywords | 転写後調節 / RNA-seq / small RNA |
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| Outline of Final Research Achievements |
In order to grow in various environments, bacteria appropriately regulate gene expression, and understanding the mechanism is important for preventing bacterial infections. It has been revealed that the 3'UTR of some mRNAs has the function of regulating gene expression, similar to small RNAs (sRNAs) that control post-transcriptional regulation in bacteria. In this study, we aimed to elucidate the function of mRNA 3'UTR in Salmonella and Vibrio cholerae. We comprehensively extracted pairs of transcripts, such as sRNA, mRNA, and tRNA, that form base pairs with mRNA 3'UTR using a new RNA-seq method and analyzed their functions.
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| Free Research Field |
細菌学、分子生物学
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| Academic Significance and Societal Importance of the Research Achievements |
大腸菌、サルモネラ、コレラ菌などの病原性グラム陰性細菌において、mRNA塩基配列から推測された複数の機能性3’UTRを同定した。特にグルタミン合成酵素をコードするglnAから大腸菌、サルモネラそれぞれ異なる塩基配列を持つsRNA GlnZが生成することを明らかにした。窒素飢餓条件でグルタミン合成酵素が発現する際に、そのmRNA 3´UTRからsRNA GlnZが生成し、2-オキソグルタル酸デヒドロゲナーゼの発現を抑制することが明らかになった。また、コレラ菌のsRNA GcvBと塩基対形成する複数のmRNA 3’UTRがGcvBの制御能を抑制するスポンジRNAとして機能することを明らかにした。
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