Molecular Mechanisms of Protein Sorting in Membrane Traffic and Roles in Higher Plants

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20001009
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: 中野 明彦 The University of Tokyo, 大学院・理学系研究科, 教授 (90142140)
• Project Period (FY): 2008 – 2013
• Keywords: 膜交通 / 選別輸送 / ライブイメージング / 出芽酵母 / シロイヌナズナ

Research Abstract

(1)膜交通の選別分子機構
主に出芽酵母を材料に,可視化の新たな方法論を積極的に開発し,選別輸送の分子機構の徹底的な理解を目指す。
(2)生化学的再構成と1分子観察
COPII小胞形成完全再構成系を用い,1分子観察を駆使して選別分子機構を解明する。
(3)高等植物における膜交通の役割
主にシロイヌナズナを材料に,膜交通が組織レベル,個体レベルでの高次機能で果たす役割を理解する。

Research Products

• [Journal Article] Sed4p stimulates Sarlp GTP hydrolysis and promotes limited coat disassembly (2011)
  • Author(s): C.Kodera, T.Yorimitsu, A.Nakano, K.Sato
  • Journal Title: Traffic 12 Pages: 591-599
• [Journal Article] Loss-of-function mutations of retromer large subunits suppress the phenotype of zig mutant that lacks Qb-SNARE VTI11 (2010)
  • Author(s): Y.Hashiguchi, M.Niihama, T.Takahashi, C. Saito, A.Nakano, M.Tasaka, M.T.Morita
  • Journal Title: Plant Cell 22 Pages: 159-172
• [Journal Article] Structural basis for the Rhol- and phosphoinositide- dependent localization of Sec3, a subunit of the exocyst complex (2010)
  • Author(s): M.Yamashita, K.Kurokawa, Y.Sato, A. Yamagata, H.Mimura, A.Yoshikawa, K.Sato, A.Nakano, S.Fukai
  • Journal Title: Nat.Struct.Mol.Biol 17 Pages: 180-186
• [Journal Article] Arabidopsis dynamin-related proteins DRP2B and DRP1A participate together in clathrin-coated vesicle formation during endocytosis (2010)
  • Author(s): M.Fujimoto, S.Arimura, T.Ueda, H.Takanashi, Y.Hayashi, A.Nakano, N.Tsutsumi
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A 107 Pages: 6094-6099
• [Journal Article] DP-bound and nucleotide-free intermediates of the guanine nucleotide exchange in the Rab5/Vps9 system (2010)
  • Author(s): T.Uejima, K.Ihara, T.Goh, E.Ito, M.Sunada, T. Ueda, A.Nakano, S.Wakatsuki
  • Journal Title: J.Biol.Chem 285 Pages: 36689-36697
• [Journal Article] Vacuolar/pre-vacuolar compartment Qa-SNAREs VAM3/SYP22 and PEP12/SYP21 have interchangeable functions in Arabidopsis (2010)
  • Author(s): T.Uemura, M.T.Morita, K.Ebine, Y.Okatani, D. Yano, C.Saito, T.Ueda, A.Nakano
  • Journal Title: Plant J 64 Pages: 864-873
• [Journal Article] ADP-ribosylation factor machinery mediates endocytosis in plant cells (2010)
  • Author(s): S.Naramoto, J.Kleine-Vehn, S.Robert, M. Fujimoto, T.Dainobu, T.Paciorek, T.Ueda, A. Nakano, M.C.E.Van Montagua, H.Fukuda, J.Friml
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A 107 Pages: 21890-21895
• [Journal Article] Passage through the Golgi (2010)
  • Author(s): A.Nakano , A.Luini
  • Journal Title: Curr.Opin.Cell Biol 22 Pages: 471-478
• [Journal Article] Phosphoisositide-dependent regulation of VAN3 ARF-GAP localization and activity essential for vascular tissue continuity in plants (2009)
  • Author(s): S.Naramoto, S.Sawa, K.Koizumi, T.Uemura, T. Ueda, J.Friml, A.Nakano, H.Fukuda
  • Journal Title: Development 136 Pages: 1529-1538
• [Journal Article] Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane (2009)
  • Author(s): K.V.Tabata, K.Sato, T.Ide, T.Nishizaka, A. Nakano, H.Noji
  • Journal Title: EMBO J. 28 Pages: 3279-3289
• [Journal Article] Generation of cell polarity in plants links endocytosis (2008)
  • Author(s): P.Dhonukshe, H.Tanaka, T.Goh, K.Ebine, A.P. Mahonen, K.Prasad, I.Blilou, N.Geldner, J. Xu, T.Uemura, J.Chory, T.Ueda, A.Nakano, B.Scheres, J.Friml
  • Journal Title: auxin gradient and cell-fate decisions.Nature 456 Pages: 962-966
• [Journal Article] A SNARE complex unique to seed plants is required for protein storage vacuole biogenesis and seed development of Arabidopsis thaliana (2008)
  • Author(s): K.Ebine, Y.Okatani, T.Uemura, T.Goh, K. Shoda, M.Niihama, M., T.Morita, C.Spitzer, M.S.Otegui, A.Nakano, T.Ueda
  • Journal Title: Plant Cell 20 Pages: 3006-3021
• [Presentation] Mechanistic insights into the membrane trafficking through and around the Golgi apparatus (2011)
  • Author(s): A.Nakano
  • Organizer: KITP Conf.on Evolutionary Perspectives on Mechanisms of Cellular Organization
  • Place of Presentation: Univ.of California. Santa Barbara, CA, USA(invited speaker)
  • Year and Date: 2011-07-20
• [Presentation] ER structure regulates the organization of COPII assembly sites and thus the biogenesis of the Golgi apparatus Special Symposium Biochemistry of Membrane Traffic : Secretory and Endocytic Pathways (2010)
  • Author(s): A.Nakano
  • Organizer: The American Society for Biochemistry and Molecular Biology
  • Place of Presentation: Tahoe City, CA, USA
  • Year and Date: 2010-10-28
• [Presentation] Golgi and post-Golgi traffic as revealed by uniquely evolved plant systems (2009)
  • Author(s): A.Nakano
  • Organizer: Gordon Research Conference on Molecular Membrane Biology
  • Place of Presentation: Andover, NH, USA, (invited speaker)
  • Year and Date: 2009-07-08
• [Presentation] Maturing cisterna (2009)
  • Author(s): A.Nakano
  • Organizer: Golgi consensus meeting.CRG, Barcelona
  • Place of Presentation: Spain(invited speaker)
  • Year and Date: 2009-06-13
• [Book] Yeast Golgi apparatus In The Golgi Apparatus(State of the art 110 years after Camillo Golgi's discovery)(eds.A.Mironov , M.Pavelka) (2008)
  • Author(s): Akihiko Nakano
  • Total Pages: 623-629
  • Publisher: Springer-Verlag,Wien
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/hasseipl/HP/index.html