Molecular Mechanisms of Protein Sorting in Membrane Traffic and Roles in Higher Plants

2013 Fiscal Year Final Research Report

• Project/Area Number: 20001009
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: NAKANO Akihiko 東京大学, 大学院・理学系研究科, 教授 (90142140)
• Co-Investigator(Kenkyū-buntansha): UEMURA Tomohiro 東京大学, 大学院・理学系研究科, 助教 (90415092) ; SATO Ken 東京大学, 大学院・総合文化研究科, 准教授 (00303602) ; ABE Hiroshi 独立行政法人理化学研究所, 応用研究開発室生物照射チーム, 先任技師 (90201897) ; HIRATA Ryogo 独立行政法人理化学研究所, 吉田化学遺伝学研究室, 先任研究員 (60260197) ; SAITO Chieko 東京大学, 大学院理学系研究科（理学部）, 特任准教授 (10321762) ; KUROKAWA Kazuo 独立行政法人理化学研究所, ライブセル分子イメージング研究チーム, 専任研究員 (40333504) ; TOMINAGA Motoki 独立行政法人理化学研究所, ライブセル分子イメージング研究チーム, 専任研究員 (50419892)
• Co-Investigator(Renkei-kenkyūsha): UEDA Takashi 東京大学, 大学院・理学系研究科, 准教授 (10311333)
• Project Period (FY): 2008 – 2013
• Keywords: 膜交通 / 選別輸送 / ライブイメージング / 出芽酵母 / シロイヌナズナ

Research Abstract

Problems of intracellular membrane traffic have been addressed by biochemical, genetic and cutting-edge live imaging approaches. Novel mechanisms of protein sorting, such as the safe and efficient delivery of cargo proteins in vesicles to the next compartment, have been elucidated. Extension of studies from yeast to higher plants has unveiled another new set of mechanisms that plants have evolved, and has given us clues to understand complex phenomena in Golgi stack assembly and post-Golgi traffic, which were confused in previous studies.

Research Products

• [Journal Article] Contact of cis-Golgi with ER exit sites executes cargo capture and delivery from the ER (2014)
  • Author(s): K. Kurokawa, M. Okamoto, and A. Nakano
  • Journal Title: Nat. Commun Volume: 5 Pages: 3653
  • DOI: doi:10.1038/ncomms4653
  • Peer Reviewed
• [Journal Article] Dynamic behavior of the trans-Golgi network in root tissues of Arabidopsis revealed by super-resolution live imaging (2014)
  • Author(s): T. Uemura, Y. Suda, T. Ueda, and A. Nakano
  • Journal Title: Plant Cell Physiol Volume: 55 Pages: 694-670
  • DOI: doi:10.1093/pcp/pcu010
  • Peer Reviewed
• [Journal Article] Isoform-selective oligomer formation of Saccharomyces cerevisiae p24 family proteins (2013)
  • Author(s): R. Hirata, C. Nihei, and A. Nakano
  • Journal Title: J. Biol. Chem Volume: 288 Pages: 37057-37070
  • DOI: doi:10.1074/jbc.M113.518340
  • Peer Reviewed
• [Journal Article] Rab GAP cascade regulates dynamics of Ypt6 during the Golgi maturation (2013)
  • Author(s): Y. Suda, K. Kurokawa, R. Hirata, and A. Nakano
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 110 Pages: 18976-18981
  • DOI: doi:10.1073/pnas. 1308627110
  • Peer Reviewed
• [Journal Article] Cytoplasmic streaming velocity as a plant size determinant (2013)
  • Author(s): M. Tominaga, A. Kimura, E. Yokota, T. Hamaguchi, T. Shimmen, K. Yamamoto, A. Nakano, and K. Ito
  • Journal Title: Dev. Cell Volume: 27 Pages: 45-352
  • DOI: doi:10.1016/j.devcel.2013.10.005
  • Peer Reviewed
• [Journal Article] Live cell visualization of Golgi membrane dynamics by super-resolution confocal live imaging microscopy (2013)
  • Author(s): K. Kurokawa, M. Ishii, Y. Suda, A. Ichihara, and A. Nakano
  • Journal Title: Methods Cell Biol Volume: 118 Pages: 235-242
  • DOI: doi:10.1016/B978-0- 12-417164-0.00014-8
  • Peer Reviewed
• [Journal Article] Distinct RABA members act in multiple steps of subcellular trafficking of the FLAGELLIN SENSING 2 receptor (2013)
  • Author(s): S. Choi, T. Tamaki, K. Ebine, T. Uemura, T. Ueda, and A. Nakano
  • Journal Title: Plant Cell Volume: 25 Pages: 1174-1187
  • DOI: doi:10.1105/tpc.112.108803
  • Peer Reviewed
• [Journal Article] Arabidopsis RABA1 GTPases are involved in transport between the trans-Golgi network and the plasma membrane, and are required for salinity stress tolerance (2013)
  • Author(s): R. Asaoka, T. Uemura, J. Ito, M. Fujimoto, E. Ito, T. Ueda, and A. Nakano
  • Journal Title: Plant J Volume: 73 Pages: 240-249
  • DOI: doi:10.1111/tpj.12023
  • Peer Reviewed
• [Journal Article] cis-Golgi proteins accumulate near the ER exit sites and act as the scaffold for Golgi regeneration after brefeldin A treatment in tobacco BY-2 cells (2012)
  • Author(s): Y. Ito, T. Uemura, K. Shoda, M. Fujimoto, T. Ueda, and A. Nakano
  • Journal Title: Mol. Biol. Cell Volume: 23 Pages: 3203-3214
  • DOI: doi:10.1091/mbc. E12-01-0034
  • Peer Reviewed
• [Journal Article] Insight into structural and regulatory roles of Sec16 in COPII vesicle formation at ER exit sites (2012)
  • Author(s): T. Yorimitsu and K. Sato
  • Journal Title: Mol. Biol. Cell Volume: 23 Pages: 2930-2942
  • DOI: doi:10.1091/mbc.E12-05-0356
  • Peer Reviewed
• [Journal Article] High-curvature domains of the endoplasmic reticulum (ER) are important for the organization of ER exit sites in Saccharomyces cerevisiae (2012)
  • Author(s): M. Okamoto, K. Kurokawa, K. Matsuura-Tokita, C. Saito, R. Hirata, and A. Nakano
  • Journal Title: J. Cell Sci Volume: 125 Pages: 3412-3420
  • DOI: doi:10.1242/jcs.100065
  • Peer Reviewed
• [Journal Article] Qa-SNAREs localized to the trans-Golgi network regulate multiple transport pathways and extracellular disease resistance in plants (2012)
  • Author(s): T. Uemura, H. Kim, C. Saito, K. Ebine, T. Ueda, P. Schulze-Lefert, and A. Nakano
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Pages: 1784-1789
  • DOI: doi: 10.1073/pnas.1115146109
  • Peer Reviewed
• [Journal Article] The occurrence of bulbs, a complex configuration of the vacuolar membrane, is affected by mutations vacuolar SNARE and phospholipase in Arabidopsis (2011)
  • Author(s): C. Saito, T. Uemura, C. Awai, M. Tominaga, K. Ebine, J. Ito, T. Ueda, H. Abe, M. T. Morita, M. Tasaka, and A. Nakano
  • Journal Title: Plant J Volume: 68 Pages: 64-73
  • DOI: doi:10.1111/j.1365-313X.2011.04665.x
  • Peer Reviewed
• [Journal Article] A membrane trafficking pathway regulated by the plant-specific RAB GTPase ARA6 (2011)
  • Author(s): K. Ebine, M. Fujimoto, Y. Okatani, T. Nishiyama, T. Goh, E. Ito, T. Dainobu, A. Nishitani, T. Uemura, M. H. Sato, H. Thordal-Christensen, N. Tsutsumi, A. Nakano, and T. Ueda
  • Journal Title: Nat. Cell Biol Volume: 13 Pages: 853-859
  • DOI: doi:10.1038/ncb2270
  • Peer Reviewed
• [Journal Article] Sed4p stimulates Sarlp GTP hydrolysis and promotes limited coat disassembly (2011)
  • Author(s): C. Kodera, T. Yorimitsu, A. Nakano, and K. Sato
  • Journal Title: Traffic Volume: 12 Pages: 591-599
  • DOI: doi:10.1111/j.1600-0854.2011. 01173.x
  • Peer Reviewed
• [Journal Article] ADP-ribosylation factor machinery mediates endocytosis in plant cells (2010)
  • Author(s): S. Naramoto, J. Kleine-Vehn, S. Robert, M. Fujimoto, T. Dainobu, T. Paciorek, T. Ueda, A. Nakano, M. C. E. Van Montagua, H. Fukuda, and J. Friml
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 107 Pages: 21890-21895
  • DOI: doi:10.1073/pnas.1016260107
  • Peer Reviewed
• [Journal Article] Arabidopsis dynamin-related proteins DRP2B and DRP1A participate together in clathrin-coated vesicle formation during endocytosis (2010)
  • Author(s): M. Fujimoto, S. Arimura, T. Ueda, H. Takanashi, Y. Hayashi, A. Nakano, and N. Tsutsumi
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 107 Pages: 6094-6099
  • DOI: doi:10.1073/pnas.0913562107
  • Peer Reviewed
• [Journal Article] Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane (2009)
  • Author(s): K. V. Tabata, K. Sato, T. Ide, T. Nishizaka, A. Nakano, and H. Noji
  • Journal Title: EMBO J Volume: 28 Pages: 3279-3289
  • DOI: doi:10.1038/emboj.2009.269
  • Peer Reviewed
• [Journal Article] Plant vacuolar trafficking occurs through distinctly regulated pathways
  • Author(s): K. Ebine, T. Inoue, J. Ito, E. Ito, T. Uemura, T. Goh, H. Abe, K. Sato, A. Nakano, and T. Ueda
  • Journal Title: Curr. Biol Volume: (in press)
  • Peer Reviewed
• [Presentation] Plant TGNs : dynamics and physiological functions (2013)
  • Author(s): T. Uemura and A. Nakano
  • Organizer: Golgi Apparatus Symposium
  • Place of Presentation: Bad Ischl, Austria
  • Year and Date: 2013-09-19
• [Presentation] Live imaging of cargo delivery from the ER to the Golgi apparatus (2013)
  • Author(s): A. Nakano and K. Kurokawa
  • Organizer: Golgi Apparatus Symposium
  • Place of Presentation: Bad Ischl, Austria
  • Year and Date: 2013-09-17
• [Presentation] Understanding mechanisms of membrane traffic by live imaging (2013)
  • Author(s): A. Nakano
  • Organizer: International Workshop on Plant Membrane Biology XVI
  • Place of Presentation: Kurashiki, Japan
  • Year and Date: 2013-03-26
• [Presentation] Super-resolution confocal live imaging microscopy (SCLIM) - a cutting-edge approach to understanding mechanisms of membrane traffic (2012)
  • Author(s): A. Nakano
  • Organizer: 2^<nd> POSTECH International Symposium on Bioimaging
  • Place of Presentation: Pohang, Korea
  • Year and Date: 2012-11-01
• [Presentation] Regulation of membrane trafficking by a variety of GTPases (2012)
  • Author(s): A. Nakano
  • Organizer: 10^<th> International Congress on Plant Molecular Biology
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2012-10-24
• [Presentation] Diversification of post-Golgi trafficking pathways among land plants (2011)
  • Author(s): T. Ueda
  • Organizer: XVIII International Botanical Congress
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2011-07-23
• [Presentation] High-resolution live imaging of cargo delivery from the ER exit site to the Golgi apparatus (2011)
  • Author(s): A. Nakano, K. Kurokawa and M. Okamoto
  • Organizer: Gordon Research Conference on Molecular Membrane Biology
  • Place of Presentation: Andover, NH, USA
  • Year and Date: 2011-07-12
• [Presentation] ER structure regulates the organization of COPII assembly sites and thus the biogenesis of the Golgi apparatus (2010)
  • Author(s): A. Nakano
  • Organizer: Special Symposium "Biochemistry of Membrane Traffic : Secretory and Endocytic Pathways," The American Society for Biochemistry and Molecular Biology
  • Place of Presentation: Tahoe City, CA, USA
  • Year and Date: 2010-10-28
• [Presentation] Mechanistic insights into the membrane trafficking through and around the Golgi apparatus (2010)
  • Author(s): A. Nakano
  • Organizer: Swiss Yeast Conference
  • Place of Presentation: Fribourg, Switzerland
  • Year and Date: 2010-09-07
• [Presentation] Mechanistic insights into the membrane trafficking through and around the Golgi apparatus (2010)
  • Author(s): A. Nakano
  • Organizer: Conference on Evolutionary Perspectives on Mechanisms of Cellular Organization
  • Place of Presentation: Kavli Institute for Theoretical Physics, Univ. of California. Santa Barbara, CA, USA
  • Year and Date: 2010-01-20
• [Presentation] Golgi and post-Golgi traffic as revealed by uniquely evolved plant systems (2009)
  • Author(s): A. Nakano
  • Organizer: Gordon Research Conference on Molecular Membrane Biology
  • Place of Presentation: Andover, NH, USA
  • Year and Date: 2009-07-08
• [Book] The role of Rab GTPases and SNARE proteins in plant endocytosis and post-Golgi trafficking. In Endocytosis in Plants (2012)
  • Author(s): T. Ueda, M. H. Sato, and T. Uemura
  • Total Pages: 201-216
  • Publisher: Ed. J. Samaj, Springer, Berlin, Germany
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/hasseipl/HP/index.html
• [Patent(Industrial Property Rights)] 成長増強植物及びその作出方法 (2012)
  • Inventor(s): 富永基樹、伊藤光二
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 特許, 13/344574
  • Filing Date: 2012-01-05
  • Overseas