2010 Fiscal Year Final Research Report
Development of a new mass fragmentation analysis for understanding the diversity of metabolite
| Project/Area Number |
20200062
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
Applied biochemistry
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| Research Institution | Kazusa DNA Research Institute |
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Principal Investigator |
SUZUKI Hideyuki Kazusa DNA Research Institute, 産業基盤開発研究部, 主任研究員 (80276162)
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Koh 財団法人かずさDNA研究所, 室長 (30344021)
SHIMADA Norimoto 財団法人かずさDNA研究所, プロジェクト研究員 (30409081)
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| Co-Investigator(Renkei-kenkyūsha) |
OGATA Yoshiyuki (独)理化学研究所, 植物科学研究センター, 研究員 (90446542)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 代謝物多様性 / 酸化反応 / メタボロミクス / 生物種間比較 / 進化 / 網羅的解析 |
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| Research Abstract |
In order to understand the diversity of metabolites produced by organisms, we have investigated three topical studies focused on oxidative reaction of metabolite. 1) We attempted to isolate a cDNA of flavonoid 8-hydroxylase from Lotus japonicus. This is the first identification of a flavonoid 8-hydroxylase cDNA from any plant species, and also this enzyme is a novel type of flavonoid hydroxylase. 2) We established a system to analyze metabolites found in Lotus japonicus that grows under oxygen-18 isotope and light conditions (16-h light/8-h dark cycles) at 22℃ for four weeks. 3) We developed a comprehensive metabolite network system based on MS/MS fragmentation analysis to classify the structural group of metabolites.
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Research Products
(4 results)
