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2010 Fiscal Year Final Research Report

In vivo induction of brown ad ipocyte differentiation by noninvasive delivery of miRNAs into stem cell

Research Project

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Project/Area Number 20200064
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Developmental biology
Research InstitutionHokkaido University

Principal Investigator

KAJIMOTO Kazuaki  Hokkaido University, 大学院・薬学研究院, 特任准教授 (10416216)

Co-Investigator(Renkei-kenkyūsha) KATAOKA Masatoshi  独立行政法人産業技術総合研究所, 健康工学研究部門, 研究グループ長 (20224438)
TABUCHI Yoshiaki  富山大学, 生命科学先端研究センター, 准教授 (20322109)
SHINOHARA Yasuo  徳島大学, 疾患ゲノム研究センター, 教授 (60226157)
KOGURE Kentaro  京都薬科大学, 薬学部, 教授 (70262540)
Project Period (FY) 2008 – 2010
Keywords褐色脂肪細胞 / miRNA / 発生・分化 / マイクロアレイ
Research Abstract

It was found that a lot of miRNAs were up-regulated when the subcutaneous adipose-derived stem cells (ASC) differentiated into brown-like adipocytes. Although these miRNAs may be involved in modulating ASC function, up-modulations of these miRNAs did not appear to affect ASC differentiation. In addition, we succeeded to obtain the novel monoclonal antibodies specifically responsible to ASC. Furthermore, we also applied the iontophoresis to noninvasive transdermal delivery of functional nucleic acids, and succeeded the silencing of the endogenous gene expression and activation of immune response in vivo. According to this study, it was suggested that ASC differentiation into brown-like adipocyte might be regulated by not only miRNAs but also unknown factors. Thus, the ASC specific antibodies and the delivery technology established by this study were helpful for the further investigation to clarify the molecular mechanisms of ASC function.

  • Research Products

    (12 results)

All 2011 2010 2009

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (7 results)

  • [Journal Article] Noninvasive and efficient transdermal delivery of CpG-oligodeoxynucleotide for cancer immunotherapy.2011

    • Author(s)
      Kigasawa K, Kajimoto K, Nakamura T, Hama S, Kanamura K, Harashima H, Kogure K.
    • Journal Title

      J Control Release. 150

      Pages: 256-265

    • Peer Reviewed
  • [Journal Article] イオントフォレシスによる高分子医薬の皮内送達2011

    • Author(s)
      小暮健太朗, 氣賀澤郁, 濱進, 梶本和昭
    • Journal Title

      薬剤学 71(2)

      Pages: 94-98

    • Peer Reviewed
  • [Journal Article] Noninvasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis2010

    • Author(s)
      Kajimoto K, Yamamoto M, Watanabe M, Kigasawa K, Kanamura K, Harashima H, Kogure K.
    • Journal Title

      Int J Pharm. 403

      Pages: 57-65

    • Peer Reviewed
  • [Journal Article] イオントフォレシスによる高分子の皮膚透過2010

    • Author(s)
      小暮健太朗, 濱進, 氣賀澤郁, 梶本和昭
    • Journal Title

      生物物理 50(4)

      Pages: 188-189

    • Peer Reviewed
  • [Journal Article] Noninvasive delivery of siRNA into the epidermis by iontophoresis using an atopic dermatitis-like model rat.2010

    • Author(s)
      Kigasawa K, Kajimoto K, Hama S, Saito A, Kanamura K, Kogure K.
    • Journal Title

      Int J Pharm. 383

      Pages: 157-160

    • Peer Reviewed
  • [Presentation] Suppression of Tumor Growth by Transdermal Delivery of Oligonucleotides via Iontophoresis.2010

    • Author(s)
      Kogure K, Kigasawa K, Hama S, Kanamura K, Kajimoto K.
    • Organizer
      The 37th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Portland, USA
    • Year and Date
      2010-07-12
  • [Presentation] 網羅的遺伝子発現解析から分かる褐色脂肪細胞と白色脂肪細胞の分化と起源2010

    • Author(s)
      梶本和昭
    • Organizer
      日本薬学会北海道支部第134回例会 総説講演
    • Place of Presentation
      札幌
    • Year and Date
      2010-05-08
  • [Presentation] 網羅的遺伝子発現解析に基づく脂肪細胞の多様性の解析2010

    • Author(s)
      梶本和昭, 白澤北斗, 小暮健太朗, 片岡正俊, 篠原康雄
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-30
  • [Presentation] Noninvasive and effective transdermal delivery of siRNA by iontophoresis.2009

    • Author(s)
      Kogure K, Kigasawa K, Kanamura K, Kajimoto K.
    • Organizer
      Joint Symposium of the 5th Annual Meeting of the Oligonucleotide Therapeutics Society and the 19th Antisense Symposium
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2009-11-04
  • [Presentation] Efficient and noninvasive transdermal delivery of siRNA by iontophoresis.2009

    • Author(s)
      Kogure K, Kigasawa K, Kanamura K, Kajimoto K.
    • Organizer
      The 36th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      2009-07-22
  • [Presentation] イオントフォレシスによるナノ粒子化siRNA皮内送達システムの構築2009

    • Author(s)
      氣賀澤郁, 梶本和昭, 斎藤顕宜, 金村聖志, 小暮健太朗
    • Organizer
      日本薬学会129年会
    • Place of Presentation
      京都
    • Year and Date
      2009-03-28
  • [Presentation] ペプチドで肥満予防~経皮デリバリーの新たな可能性~2009

    • Author(s)
      梶本和昭
    • Organizer
      TIMS/MANA Joint Seminor
    • Place of Presentation
      筑波
    • Year and Date
      2009-01-29

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Published: 2012-02-13   Modified: 2016-04-21  

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