Structure and dynamics of actin filament complex : mechanism of calcium regulation of muscle contraction

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20227008
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Nagoya University
• Principal Investigator: MAEDA Yuichiro Nagoya University, 理学研究科, 教授 (10321811)
• Project Period (FY): 2008 – 2012
• Keywords: 蛋白質の構造・動態・機能 / NMR / X線結晶解析 / 高分解能電子顕微鏡解析

Research Abstract

アクチンは筋収縮とその調節を担う。また重合・脱重合に駆動されるトレッドミリングの分子運動を介して多くの細胞機能を担う。本研究では、構造および構造動態を、細胞内・複合体レベル・分子内の3階層で解明し、それを基礎にアクチンとその結合蛋白質の離合・集散のメカニズムを解明する。それによって蛋白質分子の相互作用一般の理解を深めることを目標とする。

Research Products

• [Journal Article] Structural basis for the slow dynamics of the actin filament pointed end (2011)
  • Author(s): Narita, A., Oda, T., Ma, C.
  • Journal Title: EMBO J.
• [Journal Article] Two distinct mechanisms for actin capping protein regulation-steric and allosteric inhibition (2010)
  • Author(s): Takeda, S., Minakata, S., Koike,R.,Kawahata, I., Narita, A., Kitazawa, M., Ota, M., Yamakuni, T., Maed, Nitanai, Y.
  • Journal Title: PLoS Biol 8 Pages: e1000416
• [Journal Article] The nature of the globular to fibrous-actin transition (2009)
  • Author(s): Oda, T., Iwasa, M., Aihara, T., Masa_Y., Narita, A.
  • Journal Title: Nature 457 Pages: 441-445
• [Journal Article] Two-crystal structures of tropomyosin C-terminal fragment 176-273 : exposure of the hydrophobic core to the solvent destabilizes the tropomyosin molecule (2008)
  • Author(s): Minakata, S., Maeda, K., Oda, N., Wakabayashi, K., Nitanai, Y., Needs, X.
  • Journal Title: Biophys J. 95 Pages: 710-719
• [Journal Article] Dual roles of Glnl37 of actin revealed by recombinant human cardiac muscle alpha-actin mutants (2008)
  • Author(s): Iwasa, M., Maeda, K., Narita. A., Maed., Oda, T.
  • Journal Title: J Biol Chem 283 Pages: 21045-21053
• [Presentation] Single particle analysis for actinfilaments and olher cytoskeletal Fi1aments. (2011)
  • Author(s): NaritaA
  • Organizer: 顕微鏡学会アジア若手シンポジウム
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-05-23
• [Presentation] High resolution structure and basic properties of F-actin (2009)
  • Author(s): Madda Y, et al
  • Organizer: Inernational Union of Physiological Sciences (IUPS)
  • Place of Presentation: Kyoto, International Conference Hall
  • Year and Date: 2009-07-29
• [Presentation] Towards understanding the basic properties of F-actin (2009)
  • Author(s): Madda Y
  • Organizer: Biochemical Interactions : from molecules to Function
  • Place of Presentation: Velen (Mtlnsterland), German
  • Year and Date: 2009-04-23
• [Presentation] The Nature of G-to F-actin translation (2008)
  • Author(s): Macda Y.
  • Organizer: Gordon Research Conferences on "Muscle and molecular motors"
  • Place of Presentation: New London NH., USA
  • Year and Date: 2008-07-02
• [Book] 現代生物科学入門3構造機能生物学([細胞運動のメカニズム亅)2章 (2011)
  • Author(s): 前田雄一郎、小田俊郎
  • Total Pages: 47-109
  • Publisher: 岩波害店
• [Remarks] 2008文部科学大臣表彰・若手科学者賞(成田)
  • URL: http://str.bio.nagoya-u.ac.jp:8O8O/Plone