2012 Fiscal Year Final Research Report
Structure and dynamics of actin filament complex:mechanism of calcium regulation of muscle contraction
| Project/Area Number |
20227008
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Nagoya University |
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Principal Investigator |
MAEDA Yuichiro 名古屋大学, 理学研究科, 特任教授 (10321811)
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| Co-Investigator(Kenkyū-buntansha) |
NARITA Akihiro 名古屋大学, 理学研究科, 助教 (30360613)
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| Co-Investigator(Renkei-kenkyūsha) |
KAINOSHO Masatsune 名古屋大学, 理学研究科, 研究顧問 (20137029)
WATANABE Nobuhisa 名古屋大学, シンクロトロン光研究センター, 教授 (70212321)
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| Project Period (FY) |
2008 – 2012
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| Keywords | 細胞運動 / 細胞骨格 / アクチン・トレッドミリング / 筋収縮の調節 |
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| Research Abstract |
Actin is one of the most abundant proteins in eukaryotic cells, and preforms numerous crucial cellular functions. In muscle cells, actin monomers assemble into filamentous aggregates, play roles in muscle contraction and its regulation, while in non-muscle cells, actin drives cell motility through cycles of assembly and disassembly between monomers and filaments. In this project, the 3D structure of the actin filament was first elucidated, as well as structures of complex of actin with other proteins. Based on these structures, we have gained mechanistic understanding of actin cellular functions.
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[Journal Article] Actin branching in the initiation and maintenance of lamellipodia.2012
Author(s)
Vinzenz M., Nemethova M., Schur F., Mueller J., Narita A., Urban E., Winkler C., Schmeiser C., Koestler S. A., Rottner K., Resch G. P., Maeda Y. & Small, J. V
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Journal Title
J Cell Sci
Volume: 125
Pages: 2775-85
Peer Reviewed
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[Journal Article] Direct determination of actin polarity in the cell.2012
Author(s)
Narita, A., Mueller, J., Urban, E., Vinzenz, M., Small, J. V. & Maeda, Y
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Journal Title
J Mol Biol
Volume: 419
Pages: 359-68
Peer Reviewed
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[Journal Article] Electron microscopic visualization of the filament binding mode of actin -binding proteins.2011
Author(s)
Ito, T., Hirayama, T., Taki, M., Iyoshi, S., Dai, S., Takeda, S., Kimura-Sakiyama, C., Oda, T., Yamamoto, Y., Maeda, Y. & Narita, A.
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Journal Title
filament binding mode of actin -binding proteins. J Mol Biol
Volume: 408
Pages: 26-39
Peer Reviewed
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[Journal Article] Human spire interacts with the barbed end of the actin filament.2011
Author(s)
Ito T, Narita A, Hirayama T, Taki M, Iyoshi, S., Yamamoto, Y., Maeda, Y. & Oda, T.
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Journal Title
J Mol Biol
Volume: 408
Pages: 18-25
Peer Reviewed
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[Journal Article] Two distinct mechanisms for actin capping protein regulation--steric and allosteric inhibition.2010
Author(s)
Takeda, S., Minakata, S., Koike, R., Kawahata, I., Narita, A., Kitazawa, M., Ota, M., Yamakuni, T., Maeda, Y. & Nitanai, Y
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Journal Title
PLoS Biol
Volume: 8
Pages: e1000416
Peer Reviewed
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[Journal Article] Molecular structure of the ParM polymer and the mechanism leading to its nucleotide-driven dynamic instability.2008
Author(s)
Popp, D., Narita, A., Oda, T., Fujisawa, T., Matsuo, H., Nitanai, Y., Iwasa, M., Maeda, K., Onishi, H. & Maeda, Y
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Journal Title
EMBO J
Volume: 27
Pages: 570-9
Peer Reviewed
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[Journal Article] Two-crystal structures of tropomyosin C-terminal fragment 176-273: exposure of the hydrophobic core to the solvent destabilizes the tropomyosin molecule2008
Author(s)
Minakata, S., Maeda, K., Oda, N., Wakabayashi, K., Nitanai, Y. & Maeda, Y
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Journal Title
Biophys J
Volume: 95
Pages: 710-9
Peer Reviewed
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