2009 Fiscal Year Annual Research Report
| Project/Area Number |
20228002
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| Research Institution | Tohoku University |
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Principal Investigator |
宮澤 陽夫 Tohoku University, 大学院・農学研究科, 教授 (20157639)
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| Co-Investigator(Kenkyū-buntansha) |
仲川 清隆 東北大学, 大学院・農学研究科, 准教授 (80361145)
都築 毅 東北大学, 大学院・農学研究科, 准教授 (00404848)
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| Keywords | 過酸化脂質 / 疾病 / 食品 / 生理活性 / 分析法 / 脂質ヒドロペルオキシド |
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| Research Abstract |
過酸化脂質は食品油脂の酸化劣化として従来研究されていたが、ヒトの体内とくに生体膜脂質の過酸化が細胞老化、生活習慣病、老化性疾患に深く関与すると考え研究を展開してきた。本研究では、過酸化脂質の生成と疾病の関係を化学的定量的に究明することの重要性に着目し、生体過酸化脂質の精密一斉網羅的構造解析、過酸化脂質分析法の改良と高選択な抗体作成による汎用化、細胞障害・疾病に関わる過酸化脂質の分子機構の解明、食品による過酸化脂質の生成制御と疾病予防、これらに関する下記6課題の解明を目的とした。
(1)ビニルエーテル化合物による高純度で安定な過酸化脂質標準品の調製技術の改善と統一標準品の内外研究者への供給では、合成した種々の脂質クラスのヒドロペルオキシド標準品について、長期保存が可能な条件を検討し確立した。(2)LC-MS/MSによる生体試料(ヒト血漿、赤血球、等)の全過酸化脂質の一斉網羅的構造解析による分子種レベルでの構造全貌の解明と過酸化脂質データベースの充実では、LC-MS/MS法を活用して、動脈硬化、糖尿病、認知症における生体内酸化ストレスを最も鋭敏に反映できる過酸化脂質の脂質クラスと分子種の特定を進めている。(3)臨床応用できる簡便で選択性の高いヒドロペルオキシド型過酸化脂質の免疫定量法の開発では、高選択性な抗体の取得を目指した。(4)高脂血症、糖尿病、認知症における過酸化脂質の生理活性と関与分子機構の解明では、疾病モデル動物で過酸化脂質の生理活性と分子機構を評価している。(5)高血糖下で発見されたアマドリ型糖化脂質の簡便な免疫定量法の開発と応用では、高選択性の抗体の選定を進めた。(6)食品成分による過酸化脂質の生成と蓄積の制御による疾病予防の可能性の評価では、トコトリエノールの抗血管新生阻害機能、食品キサントフィルの赤血球脂質過酸化抑制を介した認知症進展緩和、これらについてのメカニズム解析を重点的に進めた。
このように本研究は、概ね順調に推移している。その成果は、過酸化脂質の化学生物学的特性の理解につながり、食品の新しい機能発見や疾病予防に役立つので、社会的意義と波及性が大きいといえる。
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[Journal Article] Phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to intracellular adhesion molecule-12009
Author(s)
Asai, A., Okajima, F., Nakagawa, K., Ibusuki, D., Tanimura, K., Nakajima, Y., Nagao, M., Sudo, M., Harada, T., Miyazawa. T., Oikawa, S.
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Journal Title
Journal of Lipid Research 50
Pages: 957-965
Peer Reviewed
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[Journal Article] Cross-fertilization for enhancing tocotrienol biosynthesis in rice plants and QTL analysis of their F2 progenies.2009
Author(s)
Sookwong, P., Murata, K., Nakagawa, K., Shibata, A., Kimura, T., Kojima, Y., Miyazawa, T.
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Journal Title
Journal of Agricultural and Food Chemistry 57
Pages: 4620-4625
Peer Reviewed
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[Journal Article] Antioxidant effect of lutein towards phospholipid hydroperoxidation in human erythrocytes.2009
Author(s)
Nakagawa, K., Kiko, T., Hatade, K., Sookwong, P., Arai, H., Miyazawa, T.
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Journal Title
British Journal of Nutrition. 102
Pages: 1280-1284
Peer Reviewed
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[Journal Article] Effect of co-administration of tea epigallocatechin-3-gallate(EGCG)and caffeine on absorption and metabolism of EGCG in human.2009
Author(s)
Nakagawa, K., Nakayama, K., Nakamura, M., Sookwong, P., Tsuduki, T., Niino, H., Kimura, F., Miyazawa. T.
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Journal Title
Bioscience, Biotechnology, and Biochemistry 73
Pages: 2014-2017
Peer Reviewed
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[Journal Article] δ-Tocotrienol suppresses VEGF induced angiogenesis whereas α-tocopher of does not.2009
Author(s)
Shibata, A., Nakagawa, K., Sookwong, P., Tsuduki. T., Oikawa, S., Miyazawa, T.
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Journal Title
Journal of Agricultural and Food Chemistry 57
Pages: 8696-8704
Peer Reviewed
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[Journal Article] Effect of dietary oils on host resistance to fungal infection in psychologically stressed mice.2009
Author(s)
Oarada, M., Igarashi, M., Tsuzuki, T., Kurita, N., Gonoi, T., Nikawa, T., Hirasaka, K., Miyazawa, T., Nakagawa, K, Kamei, K.
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Journal Title
Bioscience, Biotechnology, and Biochemistry 73
Pages: 1994-1998
Peer Reviewed
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[Presentation] Ion-trap tandem mass spectrometric analysis of glycated phosphatidylethanolamine in diabetic blood : evidence for lipid advanced glycation in vivo.2009
Author(s)
Shoji, N., Kato, S., Miyazawa, T., Fujita, I., Nakagawa, K., Oikawa, S., Miyazawa, T.
Organizer
International Conference on Phospholipase A2 and Lipid Mediators
Place of Presentation
東京千代田区
Year and Date
20090525-20090528
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[Presentation] Development of an ion trap tandem mass spectrometric assay for phosphatidylcholine hydroperoxide(PCOOH)molecular species in human plasma.2009
Author(s)
Yokoyama, G., Miyazawa, T., Kato, S., Ibusuki, D., Nakagawa, K., Oikawa, S., Miyazawa, T.
Organizer
International Conference on Phospholipase A2 and Lipid Mediators
Place of Presentation
東京千代田区
Year and Date
20090525-20090528
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[Presentation] Development of an ion trap tandem mass spectrometric assay for phosphatidylcholine hydroperoxide(PCOOH)molecular species in human plasma.2009
Author(s)
Yokoyama, G., Miyazawa, T., Kato, S., Ibusuki, D., Nakagawa, K., Oikawa, S., Miyazawa, T.
Organizer
The 1st International Conference on Lipid Hydroperoxide Biology and Medicine Sendai 2009
Place of Presentation
仙台市
Year and Date
2009-11-05
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[Presentation] Ion-trap tandem mass spectrometric analysis of squalene monohydroperoxide isomers in sunlight-exposed human skin.2009
Author(s)
Nakagawa, K, Ibusuki, D., Suzuki, Y, Yamashita, S., Higuchi, O., Oikawa, S., Miyazawa, T.
Organizer
The 1st International Conference on Lipid Hydroperoxide Biology and Medicine Sendai 2009
Place of Presentation
仙台市
Year and Date
2009-11-05
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[Presentation] Ion-trap tandem mass spectrometric analysis of Amadori-glycated phosphatidylethanolamine in human plasma with or without diabetes.2009
Author(s)
Nakagawa, K., Oak, J.H., Higuchi, O., Tsuzuki, T., Oikawa, S., Otani, H., Mune, M., Cai, H., Miyazawa, T.
Organizer
The 1st International Conference on Lipid Hydroperoxide Biology and Medicine Sendai 2009
Place of Presentation
仙台市
Year and Date
2009-11-05
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[Presentation] Involvement of Rac1 activation on actin polymerization and cell adhesion to intracellular adhesion molecule-1 in phsphatidylcholine hydroperoxide-treated THP-1 cells.2009
Author(s)
Asai, A., Nakagawa, K., Okajima, F., Tanimura, K., Nakajima, Y., Nagao, M., Sudo, M., Harada, T., Miyazawa, T., Oikawa, S.
Organizer
The 1st International Conference on Lipid Hydroperoxide Biology and Medicine Sendai 2009
Place of Presentation
仙台市
Year and Date
2009-11-05
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[Presentation] Oxidative stress in the absence of inflammation in a mouse model for hepatitis C virus-associated hepatocarcinogenesis.2009
Author(s)
Moriya, K, Nakagawa, K., Santa, T., Shintani, Y., Fujie, H., Miyoshi, H., Tsutsumi, T., Miyazawa, T., Ishibashi, K., Horie, T., Imai, K., Todoroki, T., Kimura, S., Koike, K.
Organizer
The 1st International Conference on Lipid Hydroperoxide Biology and Medicine Sendai 2009
Place of Presentation
仙台市
Year and Date
2009-11-05
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[Presentation] Effects of dietary fish oil on lipid peroxidation and serum triglyceride levels in psychologically stressed mice.2009
Author(s)
Oarada, M., Tsuzuki, T., Igarashi, M., Miyazawa, T., Nakagawa. K, Gonoi, T.
Organizer
The 1st International Conference on Lipid Hydroperoxide Biology and Medicine Sendai 2009
Place of Presentation
仙台市
Year and Date
2009-11-05
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[Presentation] Preparation of pure lipid hydroperoxides.2009
Author(s)
Ibusuki, D., Nakagawa, K, Asai, A., Oikawa, S., Masuda, Y., Suzuki, T., Miyazawa, T.
Organizer
The 1st International Conference on Lipid Hydroperoxide Biology and Medicine Sendai 2009
Place of Presentation
仙台市
Year and Date
2009-11-05
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[Presentation] Telomerase inhibition, telomere shortening, and senescence of DLD-1 human colorectal adenocarcinoma cells by tocotrienol.2009
Author(s)
Eitsuka, T., Nakagawa. K., Miyake, N., Kurata, T., Miyazawa, T.
Organizer
The 1st International Conference on Lipid Hydroperoxide Biology and Medicine Sendai 2009
Place of Presentation
仙台市
Year and Date
2009-11-05
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[Presentation] Tocotrienol inhibits secretion of angiogenic factors from human colorectal adenocarcinoma cells by suppressing hypoxia-inducible factor-1α.2009
Author(s)
Shibata, A., Nakagawa, K., Sookwong, P., Tsuduki, T., Tomita, S., Shirakawa, H., Komai, M., Miyazawa, T.
Organizer
The 1st International Conference on Lipid Hydroperoxide Biology and Medicine Sendai 2009
Place of Presentation
仙台市
Year and Date
2009-11-05
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