Studies on lipid peroxidation in human disease: its modulation from the view point of food chemistry

2012 Fiscal Year Final Research Report

• Project/Area Number: 20228002
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: Tohoku University
• Principal Investigator: MIYAZAWA Teruo 東北大学, 大学院・農学研究科, 教授 (20157639)
• Co-Investigator(Kenkyū-buntansha): NAKAGAWA Kiyotaka 東北大学, 大学院農学研究科, 准教授 (80361145) ; TSUDUKI Tsuyoshi 東北大学, 大学院農学研究科, 准教授 (00404848)
• Co-Investigator(Renkei-kenkyūsha): OIKAWA Shinichi 日本医科大学, 医学部, 教授 (30142946) ; OKA Yoshitomo 東北大学, 大学院医学系研究科, 教授 (70175256) ; ARAI Hiroyuki 東北大学, 加齢医学研究所, 教授 (30261613) ; SHIMOSEGAWA Tooru 東北大学, 大学院医学系研究科, 教授 (90226275) ; KIMURA Fumiko 東北大学, 大学院農学研究科, 助教た (50321980)
• Project Period (FY): 2008 – 2012
• Keywords: 過酸化脂質 / 脂質ヒドロペルオキシド / 動脈硬化症 / 認知症 / 食品機能

Research Abstract

Oxidation of lipids generates lipid hydroperoxide. Through the quantitative analysis of clinical sample with CL-HPLC and LC-MS/MS, and using the functional assessment with cell culture study and animal study, we have proved that the hydroperoxidation of biological lipid results functional deterioration of cells and tissues. Our results also indicated the lipid hydroperoxide of body causes the related disorders like arteriosclerosis and dementia. Antioxidative food components; vitamin E, polyphenol, carotenoid, suppress lipid oxidation in vivo and are useful as antisenescence-nutrient.

Research Products

• [Journal Article] Lipid glycation and protein glycation in diabetes and atherosclerosis (2012)
  • Author(s): Miyazawa, T., Nakagawa, K., Shimasaki, S., Nagai, R.
  • Journal Title: Amino Acids Volume: 42 Pages: 1163-1170
  • DOI: DOI:10.1007/s00726-010-0772-3.
  • Peer Reviewed
• [Journal Article] Significance of lutein in red blood cells of Alzheimer's disease patients. (2012)
  • Author(s): Kiko T., Nakagawa K., Tsuduki T., Suzuki T., Arai H., Miyazawa T.
  • Journal Title: J. Alzheimers Dis. Volume: 28 Pages: 593-600
  • DOI: DOI:10.3233/JAD-2011-111493
  • Peer Reviewed
• [Journal Article] Amador-glycated phosphatidyl ethanolamine up -regulates telomerase activity in PANC-1 human pancreatic carcinoma cells (2012)
  • Author(s): Eitsuka T., Nakagawa K., Ono Y., Tatewaki N., Nishida H., Kurata T., Shoji N., Miyazawa T.
  • Journal Title: FEBS Lett. Volume: 586 Pages: 2542-2547
  • DOI: DOI:10.1016/j.febslet.2012.06.027.
  • Peer Reviewed
• [Journal Article] Amyloid β levels in human red blood cells (2012)
  • Author(s): Kiko T., Nakagawa K., Satoh A., Tsuduki T., Furukawa K., Arai H., Miyazawa T.
  • Journal Title: PLoS One Volume: 7 Pages: e49620
  • DOI: DOI:10.1371/journal.pone.0049620.
  • Peer Reviewed
• [Journal Article] Miyazawa, T. Antioxidant effect of astaxanthin on phospholipid peroxidation in human erythrocytes (2011)
  • Author(s): Nakagawa K., Kiko T., Miyazawa T., Burdeos G.C., Kimura F., Satoh A.
  • Journal Title: Brit. J. Nutr. Volume: 105 Pages: 1563-1571
  • DOI: DOI:10.1017/S0007114510005398
  • Peer Reviewed
• [Journal Article] Involvement of Rac GTPase activation in phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to ICAM-1 (2011)
  • Author(s): Asai A. Okajima F., Nakajima Y., Nagao M., Nakagawa K., Miyazawa T., Oikawa S.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 406 Pages: 273-277
  • DOI: DOI:10.1016/j.bbrc.2011.02.032
  • Peer Reviewed
• [Journal Article] Amyloid β-induced erythrocytic damage and its attenuation by carotenoids (2011)
  • Author(s): Nakagawa, K., Kiko, T., Miyazawa Taiki, Sookwong, P., Tsuduki, T. Satoh, A., Miyazawa, T.
  • Journal Title: FEBS Lett. Volume: 585 Pages: 1249-1254
  • DOI: DOI:10.1016/j.febslet.2011.03.060
  • Peer Reviewed
• [Journal Article] Phosphatidyl choline hydroperoxide promotes VEGF-induced angiogenesis in endothelial cells and rat aorta ring cultures (2011)
  • Author(s): Nakagawa K., Shibata A., Saito T., Sookwong P., Kato S., Tsuduki T., Matsubara K., Miyazawa T.
  • Journal Title: Biochim. Biophys. Acta Volume: 1810 Pages: 1205-1211
  • DOI: doi:10.1016/j.bbagen.2011.08.018
  • Peer Reviewed
• [Journal Article] LC-MS/MS analysis of carboxymethylated and carboxy -ethylated phosphatidylethanolamines in human erythrocytes and blood plasma (2010)
  • Author(s): Shoji N., Nakagawa K., Asai A., Fujita I., Hashiura, A. Nakajima Y., Oikawa S., Miyazawa T.
  • Journal Title: J. Lipid Res. Volume: 51 Pages: 2445-2453
  • Peer Reviewed
• [Journal Article] α-Tocopherol attenuates the cytotoxic effect of δ-tocotrienol in human colorectal adenocarcinoma cells (2010)
  • Author(s): Shibata A., Nakagawa K., Sookwong, P. Tsuduki T., Asai A., Miyazawa T.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 397 Pages: 214-219
  • DOI: DOI:10.1016/j.bbrc.2010.05.087
  • Peer Reviewed
• [Journal Article] Sulforaphane suppresses UVB induced inflammation in HaCaT keratinocytes and HR-1 hairless mice (2010)
  • Author(s): Shibata A., Nakagawa K., Yamanoi H., Tsuduki T., Sookwong P., Higuchi O., Kimura F., Miyazawa T.
  • Journal Title: J. Nutr. Biochem. Volume: 21 Pages: 702-709
  • DOI: DOI:10.1016/j.jnutbio.2009.04.007.
  • Peer Reviewed
• [Journal Article] Miyazawa T., γ-Tocotrienol reduces squalene hydroperoxide-induced inflammatory responses in HaCat keratinocytes (2010)
  • Author(s): Nakagawa K., Shibata A., Maruko T., Sookwong P., Tsuzuki T., Nakayama K., Nishida H.
  • Journal Title: Lipids Volume: 45 Pages: 833-841
  • DOI: DOI:10.1007/s11745-010-3458-4.
  • Peer Reviewed
• [Journal Article] Antiangiogenic and anticancer potential of unsaturated vitamin E, tocotrienol (2009)
  • Author(s): Miyazawa T., Shibata A., Sookwong P., Kawakami Y., Eitsuka T., Asai A., Oikawa S., Nakagawa K.
  • Journal Title: J. Nutr. Biochem. Volume: 20 Pages: 79-86
  • DOI: DOI:10.1016/j.jnutbio.2008.09.003.
  • Peer Reviewed
• [Journal Article] Phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to intracellular adhesion molecule-1 (2009)
  • Author(s): Asai A., Okajima F., Nakagawa K., Ibusuki D., Tanimura K., Nakajima Y., Nagao M., Sudo M., Harada T., Miyazawa T., Oikawa S.
  • Journal Title: J. Lipid Res. Volume: 50 Pages: 957-965
  • DOI: DOI:10.1194/jlr.M800582-JLR200.
  • Peer Reviewed
• [Journal Article] Antioxidant effect of lutein towards phospholipid hydroperoxidation in human erythrocytes (2009)
  • Author(s): Nakagawa K., Kiko T., Hatade K., Sookwong P., Arai H., Miyazawa T.
  • Journal Title: Brit. J. Nutr. Volume: 102 Pages: 1280-1284
  • DOI: DOI:10.1017/S0007114509990316.
  • Peer Reviewed
• [Journal Article] Preparation of pure lipid hydroperoxides (2008)
  • Author(s): Ibusuki D., Nakagawa K., Asai A., Oikawa S., Masuda Y., Suzuki T., Miyazawa T.
  • Journal Title: J. Lipid Res. Volume: 49 Pages: 2668-2677
  • DOI: DOI:10.1194/jlr.D800034-JLR200.
  • Peer Reviewed
• [Journal Article] Tocotrienol inhibits secretion of angiogenic factors from human colorectal adenocarcinoma cells via suppressing hypoxia-inducible factor-1α (2008)
  • Author(s): Shibata A., Nakagawa K., Sookwong P., Tsuzuki T., Tomita S., Shirakawa H., Komai M., Miyazawa T.
  • Journal Title: J. Nutr. Volume: 138 Pages: 2136-2142
  • DOI: DOI:10.3945/jn.108.093237
  • Peer Reviewed
• [Journal Article] Development of a high-performance liquid chromatography-based assay for carotenoids in human red blood cells: Application to clinical studies (2008)
  • Author(s): Nakagawa K., Kiko T., Hatade K., Asai A., Kimura F., Sookwong P., Tsuzuki T., Arai H., Miyazawa T.
  • Journal Title: Anal. Biochem. Volume: 381 Pages: 129-134
  • DOI: DOI:10.1016/j.ab.2008.06.038
  • Peer Reviewed
• [Journal Article] Tumor anti-angiogenic effect and mechanism of action of δ-tocotrienol (2008)
  • Author(s): Shibata A., Nakagawa K., Sookwong P., Tsuzuki T., Oikawa S., Miyazawa T.
  • Journal Title: Biochem. Pharmacol. Volume: 76 Pages: 330-339
  • DOI: DOI:10.1016/j.bcp.2008.05.017.
  • Peer Reviewed
• [Journal Article] Analysis of Amadori-glycated phosphatidylethanolamine in the plasma of healthy subjects and diabetic patients by liquid chromatography-tandem mass spectrometry (2008)
  • Author(s): Miyazawa T., Ibusuki D., Yamashita S., Nakagawa K.
  • Journal Title: Ann. N. Y. Acad. Sci. Volume: 1126 Pages: 291-294
  • DOI: DOI:10.1196/annals.1433.033.
  • Peer Reviewed
• [Presentation] 血中過酸化リン脂質のMS/MS分析における注意点と解決法 (2012)
  • Author(s): 仲川清隆, 加藤俊治, 宮澤陽夫
  • Organizer: 第12回基準油脂分析試験法セミナー
  • Place of Presentation: 東京
  • Year and Date: 20121126-27
• [Presentation] Amadori-glycated, caboxy-methylated and carboxyethylated phosphatidyl -ethanol-amines in human erythrocytes and plasma (2012)
  • Author(s): Teruo Miyazawa
  • Organizer: 11th International Symposium on the Maillard Reaction
  • Place of Presentation: フランス ナンシー
  • Year and Date: 20120916-20
• [Presentation] 過酸化脂質研究からみた食品機能成分 (2011)
  • Author(s): 宮澤陽夫
  • Organizer: 日本食品科学工学会第58回大会
  • Place of Presentation: 仙台
  • Year and Date: 20110909-11
• [Presentation] Lutein and astaxanthin prevent lipid oxidation in erythrocyte membrane as being characteristic alzheimer disease (2011)
  • Author(s): Taiki Miyazawa, Takehiro Kiko, Kiyotaka Nakagawa, Hiroyuki Arai,Teruo Miyazawa
  • Organizer: New Developments in Carotenoids Research
  • Place of Presentation: アメリカ合衆国 ボストン
  • Year and Date: 20110311-12
• [Presentation] 生活環境下の太陽光暴露でヒト皮膚に生じるスクアレン過酸化物による皮膚炎症惹起の分子機構 (2010)
  • Author(s): 仲川清隆
  • Organizer: 日本油化学会第49回年会
  • Place of Presentation: 函館
  • Year and Date: 20100915-17
• [Presentation] 生体過酸化脂質の生成と食品成分による制御 (2010)
  • Author(s): 仲川清隆
  • Organizer: 第64回日本栄養・食糧学会大会
  • Place of Presentation: 徳島
  • Year and Date: 20100521-23
• [Presentation] Overview of lipid peroxidation in food and biological systems (2009)
  • Author(s): Teruo Miyazawa
  • Organizer: The 1st International Conference on Lipid Hydroperoxide Biology and Medicine Sendai 2009
  • Place of Presentation: Sendai
  • Year and Date: 20091104-06
• [Presentation] LC-MS/MSによる脂質ヒドロペルオキシドの網羅的解析と定量 (2008)
  • Author(s): 宮澤陽夫, 仲川清隆, 指宿大悟, 浅井明, 及川眞一
  • Organizer: 第33回日本医用マススペクトル学会年会
  • Place of Presentation: 東京
  • Year and Date: 20080925-26
• [Presentation] 脂質ハイドロパーオキサイドの網羅的分子種解析 (2008)
  • Author(s): 宮澤陽夫, 指宿大悟, 山下慎司, 仲川清隆
  • Organizer: 第50回日本脂質生化学会
  • Place of Presentation: 徳島
  • Year and Date: 20080605-06
• [Presentation] Ion-trap tandem mass spectrometric analyses of blood plasma phosphatidylcholine hydroperoxides and skin squalene hydroperoxides (2008)
  • Author(s): Teruo Miyazawa
  • Organizer: A Biochemical Society Focused Meeting
  • Place of Presentation: Exeter, UK
  • Year and Date: 20080402-03
• [Book] Edited by Victor R. Preedy. The Royal Society of Chemistry (2012)
  • Author(s): Taiki Miyazawa, Kiyataka Nakagawa, Teruo Miyazawa
  • Total Pages: 184-203
  • Publisher: Chapter 12 Liquid Chromatography-based Assay for Carotenoids in Human Blood. Food and Nutritional Components in Focus No.1. Vitamin A and Carotenoids: Chemistry, Analysis, Function and Effects
• [Book] 食品機能素材IV, 太田明一監修, シーエムシー出版 (2010)
  • Author(s): 宮澤陽夫
  • Total Pages: 25-30
  • Publisher: 生体内における抗酸化作用
• [Book] 糖化による疾患と抗糖化食品・素材,シーエムシー出版ISBN978-4-7813-0294-2 (2010)
  • Author(s): 宮澤陽夫
  • Total Pages: 105-113
  • Publisher: 脂質グリケーション産物のLC-UV/MSとLC-MS/MS
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/kinoubun/index-j.html